Figure 1: Dauer recovery on E. coli OP50 under different conditions of population size and genotype. (A) Methodological outline used for recovery of dauers in the presence of corpses or live animals in plates with E. coli OP50 food. (B) Percent of dauer recovery after 24 hours of exposure to food on different proportions of cadavers and dauers (upper panel) and representation of numbers of dauers, cadavers and total population on each proportion (lower panel). (C) Percent of dauer recovery after 24 hours of exposure to food in the proportion 1:4 (6000 cadavers to 1500 dauers) on different conditions of wild type and
daf-22 genetic backgrounds. a and b represent statistically different conditions. All comparisons, significance and P value are indicated. (D) Representative pictures of plates on the first day of dauer exposure to E. coli OP50 in the presence of corpses (0 hours) and 24 hours after. The third photograph is a zoom of photographs at 24 hours to show animals that exited dauer (stars) and dauers (arrows). Bars are 200 mm. Photographs were taken with a Cannon camera EOS Rebel T3i on a dissecting Nikon AZ100 in 2X objective at 1/400s exposure time, with ISO 100, focal distance 50 mm and 5X or 8X magnification.