Fig. 3. Dynamic expression of
nlg-1 splices variants during development. (A)
nlg-1 expression during development. Animals carrying the Pnlg-1::GFP transgene were imaged at 65 and 100 times magnifications. We observed
nlg-1 promoter drive GFP expression from 1.5-fold stage into the adult. Key stages of embryonic and larval development of C. elegans are indicated on the top. For the embryonic development, samples were imaged by DIC and epifluorescence, respectively. The asterisks indicate autofluorescence in gut cells, and yellow arrowheads indicate GFP expressing cells. For the larval development cartoon of the distinct stages overlie panels showing epifluorescence images of the whole worm (upper), head region middle panel or orientated to highlight the ventral cord (lower panel) at distinct stages and magnification. The scale bars always correspond to 10 um. (B) Differential expression of
ngl-1 splice variants during C. elegans development. PCR was performed on cDNA generated from the indicated mixed or staged developmental window. During stages L1-L2 and L3 the dominant isoform corresponds to transcripts containing exon 14. However, in embryo and stages L4-adult the major neuroligin isoform corresponded to transcripts lacking exon 14.
act-2 gene was used as control gene and showed relatively equivalent expression.