Picture from Hyenne V et al. (2015) J Cell Biol "RAL-1 controls multivesicular body biogenesis and exosome secretion."

(E-H) APEX::RAL-1(DN) shows DAB staining both at apical membrane stacks (E and F, arrowheads) and at the external surface of MVBs (E and G-G'', arrows). Animals expressing no APEX tag treated with DAB show no staining (H). The star indicates a MVB. APM, apical plasma membrane.

Picture from Crittenden SL et al. (1994) Development "GLP-1 is localized to the mitotic region of the C. elegans germ line."

Figure 8. In situ hybridization of glp-1. Distal (*) and proximal (**) ends are marked. (A) Dissected adult hermaphrodite gonad hybridized with glp-1 anti-sense RNA probe. glp-1 mRNA is uniformly distributed throughout the germ line, including oocytes (arrowheads). No glp-1 mRNA is detected in sperm (arrow). (B) Dissected adult hermaphrodite gonad hybridized with glp-1 sense RNA probe. No signal is detected. (C) Dissected adult male gonad hybridized with glp-1 anti-sense RNA probe. glp-1 mRNA is most abundant distally, but decreases more proximally. No glp-1 mRNA is detected in mature sperm. D. Dissected adult male gonad hybridized with glp-1 sense RNA probe. No signal is detected. Scale bar, 10 µm.

Picture from de Carvalho CE et al. (2008) Genes Dev "LAB-1 antagonizes the Aurora B kinase in C. elegans."

Immunolocalization of LAB-1 in meiotic prophase I. No signal is detected on mature sperm (arrow in DAPI panel). Bar, 20 μm.

Picture from Lohmer LL et al. (2016) PLoS Genet "A Sensitized Screen for Genes Promoting Invadopodia Function In Vivo: CDC-42 and ...."

(B) An AC-specific full-length fusion of GFP to GDI-1 (cdh3>GFP::gdi-1) showed cytosolic distribution with no specific subcellular enrichment.

Picture from Kim KW et al. (2018) Neuron "A Neuronal piRNA Pathway Inhibits Axon Regeneration in C.elegans."

(A) smFISH shows prde-1 mRNAs in the cytoplasm of PLM neuron labeled with Pmec-7-gfp (muIs32). Right bottom shows no probe control.

Picture from Taubert S et al. (2008) PLoS Genet "The Mediator subunit MDT-15 confers metabolic adaptation to ingested ...."

Figure 4. A model for MDT-15 function. Model depicting the role of MDT-15 in monitoring availability and quality of ingested material. Note that in each case, we propose that MDT-15 confers regulatory effects both in 'unchallenged' (sufficient energy supply, no xenobiotics or metals, no pathogens) and 'challenged' (fasting, xenobiotic or metal exposure, pathogenic infection) conditions. For further explanations, see text.

Picture from Dong X et al. (2013) Cell "An extracellular adhesion molecule complex patterns dendritic branching and ...."

(G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells.

Picture from Rhind NE et al. (1995) Cell "xol-1 acts as an early switch in the C. elegans male/hermaphrodite ...."

Figure 6. Sex-Specific Expression of a xol-l::lacZ Fusion him-8(A)or wild-type(B) gravid hermaphrodites carrying an integrated xol-l::lacZ translational fusion (ylS2) were fixed and stained withX-Gal. We observed strong staining in XO embryos and weak or no staining in XX embryos from the 28-cell stage to the comma stage. No other significant embryonic or postembryonic staining was observed.

Picture from Deplancke B et al. (2006) Cell "A gene-centered C. elegans protein-DNA interaction network."

(C) PJC8.6::JC8.6:::GFP - Mosaic animals, no stable transgenic line could be obtained. GFP is broadly expressed in embryos, larvae and adults with strongest GFP expression in the pharynx and vulva.

Picture from Beydoun S et al. (2024) MicroPubl Biol "Polyethylene glycol as an improved barrier to prevent fleeing in C. ...."

Figure 1. Use of barriers to prevent fleeing in C elegans:(A) Schematic of worms on plates with food (left), no food (middle), and no food with a barrier/fence (right). (B) Representative images of palmitic acid (PA), polyethylene glycol (PEG), and copper fences (edge of barrier is shown by an arrow) placed on the edge of NGM plates. Note: PEG is not visible when it dries. (C) Representative bright-field images of wildtype fed worms following a 96-hour exposure to PA, PEG 2,000, PEG 20,000, and copper as compared to a no fence control. (D) Quantification of images in (D) to determine the effect of each fencing tool on worm size. (E) Flee rate of day 1 adult wildtype worms under 24 hours of fed and fasted conditions with PA and PEG 20,000 fences as compared to a no fence control. (F) Flee rate of day 1 adult wildtype worms after a 24-hour exposure to pathogenic bacteria Enterococcus faecalis with PA and PEG 20,000 fences as compared to a no fence control. (G) Pharyngeal pumping and (H) thrashing rates of wildtype worms after a 24-hour exposure to PA and PEG 20,000 as compared to no fence fed control. (I) Lifespan of wildtype fed and DR (bacterial deprivation, or BD) worms at 20°C with PA and PEG 20,000 fences as compared to a no fence control. (J) Quantification of flee rate during lifespans shown in (I). One-way ANOVA with Tukey Post-Hoc analysis was used to derive p-values for worm size, flee rate, pumping and thrashing comparisons. Log-rank test was used to derive p-value for lifespan comparisons. All error bars shown in the figures represent the standard error of the mean (SEM). ns, not significant, * denotes p-value < 0.05, ** denotes p-value < 0.01, *** denotes p-value <0.001 and **** denotes p-value < 0.0001.