Picture from Vairoletti, Franco et al. (2021) MicroPubl Biol

Figure 1. L1-adapted infrared motility assay provides enhanced sensitivity for nematicide discovery: A. Adapted protocol described using L1 larvae synchronized in liquid medium. B. Comparison of EC100 values obtained for Wact11 by Burns et al. (L1) and our L4 and adapted L1 assay. C. Kinetic data from a representative Wact11 dose-response experiment, Curves for vehicle (DMSO 1%) and different compound concentrations in vehicle are shown. D. Dose-response curve for Wact11 at endpoint in our adapted L1 assay. The motility ratio is defined as compound normalized motility in vehicle/vehicle motility.