Figure 5. Expression of GEM- 4::GFP in L1 larvae. (A and B) DIC and GFP fluorescence images of an early L1 stage animal of genotype
gon-2(
q388);
gem-4(
dx77); dxEx15[
gem-4::GFP; pRF4] raised at 15C [permissive temperature for
gon-2(
q388)]. (C and D) DIC and GFP fluorescence images of an early L1 stage animal of genotype
gon-2(
q388); dxEx15 [
gem-4::GFP; pRF4] raised at 25C [restrictive temperature for
gon-2(
q388)].
gem-4:: GFP is also localized to the intestinal brush border (bb). The granular/globular fluorescence in B and D is due to autofluorescence of gut granules. Bar, 10 um.