Picture from Dang H et al. (2004) Mol Biol Cell "Disease-related myotubularins function in endocytic traffic in ...."

Figure 5. Subcellular localization and interaction of MTM-6A and MTM-9. (A) High-magnification confocal images of the GFP fusion protein expressed in the coelomocytes of the following strains (Ex signifies the transgenic array from which the indicated protein is expressed): a, +; Ex[MTM-6A::GFP]; b, +; Ex[MTM-6A(C335S):: GFP]; c, mtm-9(ar479); Ex[MTM-6A(C335S)::GFP]; d, rme-1(b1045); Ex[MTM-6A(C335S)::GFP]; e, +; Ex[MTM-6AdeltaFYVE::GFP]; f, +; Ex[MTM-6A (C335S)deltaFYVE::GFP]; g, +; Ex[MTM9::GFP]; and h, Ex[MTM-6A(C335S)]; Ex[MTM9::GFP]. Bar, 5 um in all images. (B) Coimmunoprecipitation of GFP-MTM-6A and Flg-MTM-9 expressed in human 293 cells. Antibodies against GFP or Flg were used to immunoprecipitate the corresponding fusion protein indicated above the gel. Blots were probed for the presence of the other fusion protein by using the antibodies indicated below the gel.

Picture from Frand AR et al. (2005) PLoS Biol "Functional genomic analysis of C. elegans molting."

Cycles of fluorescence observed in the hypodermis and seam cells of Ex[mlt-9p::gfp-pest] (dashed line) or Ex[mlt-11p::gfp-pest] (solid line) larvae.

Picture from Offenburger SL et al. (2018) PLoS Genet "Mutations in Caenorhabditis elegans neuroligin-like glit-1, the apoptosis ...."

S6 Fig. glit-1 expression.(A) L3 stage larva expressing Ex[Pglit-1::gfp::glit-1]. (B) Embryo and L4 stage larvaexpressing Ex[Pglit-1::gfp::glit-1]. (C)-(E) Head region of the L4 stage larva from Fig2B-2F. The green channel shows expression of Ex[Pglit-1::gfp::glit-1]. The red channelshows expression of Is[Pdat-1::NLS::rfp;Pttx-3::mCherry] for labelling of dopaminergicneuron nuclei, as well as the pharynx muscle marker Ex[Pmyo-2::mCherry] and the bodymuscle marker Ex[Pmyo-3::mCherry] that were used for injections. (F) L1 stage larvaexpressing Ex[Pglit-1::gfp]. (G) and (H) Head region of adult animals. The green channelshows expression of Ex[Pglit-1::gfp]. The red channel shows expression of Is[Pdat1::NLS::rfp;Pttx-3::mCherry] for labelling of dopaminergic neuron nuclei, as well as the pharynx muscle marker Ex[Pmyo-2::mCherry] and the body muscle marker Ex[Pmyo3::mCherry] that were used for injections.

Picture from Offenburger SL et al. (2018) PLoS Genet "6-OHDA-induced dopaminergic neurodegeneration in Caenorhabditis elegans is ...."

Fig 5. Evidence that ttr-33 is expressed in the posterior arcade cells and in the pharynx. (A) Head region of L1 stagelarvae expressing the extrachromosomal transcriptional reporter Ex[Pttr-33::gfp].Arrows indicate strongly expressingcells. (B) L1 stage larva expressing the transcriptional reporter Ex[Pttr-33::gfp].(C) Pretzel stage embryo expression thetranscriptional reporter Ex[Pttr-33::gfp].The speckled signal is caused by intestinal autofluorescence.

Picture from Dang H et al. (2004) Mol Biol Cell "Disease-related myotubularins function in endocytic traffic in ...."

Subcellular localization of MTM-9. High-magnification confocal images of the GFP fusion protein expressed in the coelomocytes of +; Ex[MTM9::GFP].

Picture from Frand AR et al. (2005) PLoS Biol "Functional genomic analysis of C. elegans molting."

Ex[mlt-8p::gfp-pest] (dashed line) or Ex[mlt-10p::gfp-pest] (solid line) larvae were examined for fluorescence and for molting from late in the L1 stage until early adulthood. Graph shows the percent of worms that were fluorescent over time, on a scale normalized to the molting cycle of each worm under observation.

Picture from Offenburger SL et al. (2018) PLoS Genet "Mutations in Caenorhabditis elegans neuroligin-like glit-1, the apoptosis ...."

Fig 2. glit-1 is expressed in the pharynx, the intestine and in several cells in the head includingdopaminergic neurons. (A) L1 stage larva expressing Ex[Pglit-1::gfp::glit-1]. (B) L4 stage larva expressingEx[Pglit-1::gfp::glit-1]. This image was generated from three individual images in ImageJ using the Stitchingplugin [60]. (C)-(F) Close up of dopaminergic neurons of the same L4 stage larva. The green channel showsexpression of Ex[Pglit-1::gfp::glit-1]. The red channel shows expression of Is[Pdat-1::NLS::rfp;Pttx-3::mCherry] for labelling of dopaminergic neuron nuclei, as well as the pharynx muscle marker Ex[Pmyo-2::mCherry] and the body muscle marker Ex[Pmyo-3::mCherry] that were used for injections.

Picture from Dang H et al. (2004) Mol Biol Cell "Disease-related myotubularins function in endocytic traffic in ...."

Subcellular localization of MTM-6A. High-magnification confocal images of the GFP fusion protein expressed in the coelomocytes of +; Ex[MTM-6A::GFP].

Picture from Jiu Y et al. (2012) PLoS One "Exocyst subunits Exo70 and Exo84 cooperate with small GTPases to regulate ...."

Figure S1 Confocal image of adult hermaphrodites expressing Psec-6::GFP under the 2065 bp promoter (Ex[Psec-6::GFP; pRF4]). Left is anterior. Scale bar, 100 um.

Picture from Jiu Y et al. (2012) PLoS One "Exocyst subunits Exo70 and Exo84 cooperate with small GTPases to regulate ...."

Figure 1. Expression patterns of exocyst subunit exoc-7 in C. elegans. Confocal images of adult hermaphrodites expressing Pexoc-7::GFP under the 2954 bp promoter (Ex[Pexoc-7::GFP; pRF4]).