Picture from Bringmann H et al. (2007) Curr Biol "LET-99, GOA-1/GPA-16, and GPR-1/2 are required for aster-positioned ...."

Figure 3. The Mitotic Spindle Positions Cortical LET-99 at Anaphase but Not at Metaphase. (A) LET-99 localization. Shown is YFP::LET-99 epifluorescence during metaphase (top) and cytokinesis (bottom). White squares indicate position of aster centers, and arrowheads indicate cortical LET-99. The scale bar represents 10 μm. LET-99 localizes to the cortex, the polar body, metaphase chromosomes, and the spindle midzone. Plots display cortical fluorescence intensity versus embryo length. The anterior is to the left, and the posterior is to the right.(B and C) Spindle displacement assay. (B) A glass needle is pressed on the anterior of the cell. The spindle is hyperdisplaced to the posterior compared with wild-type embryos. (C) A glass needle is pressed on the posterior of the cell. The spindle is displaced to the anterior of the cell. Shown are YFP::LET-99 fluorescence during metaphase and cytokinesis. White squares indicate position of aster centers, and arrowheads indicate cortical LET-99. Errors are SEM. Scale bars represent 10 μm.(D) Quantification of spindle displacement. The peak of LET-99 intensity is in the middle of the embryo at metaphase, regardless of spindle position, but shifts to the position midway between the asters at anaphase. Black squares represent aster centers. The yellow histogram shows YFP::LET-99 peak maximum position, and the gray histogram shows the cytokinesis-furrow position. Errors are SEM.

Picture from Bringmann H et al. (2007) Curr Biol "LET-99, GOA-1/GPA-16, and GPR-1/2 are required for aster-positioned ...."

The Mitotic Spindle Positions Cortical LET-99 at Anaphase but Not at Metaphase. (A) LET-99 localization. Shown is YFP::LET-99 epifluorescence during metaphase (top) and cytokinesis (bottom). White squares indicate position of aster centers, and arrowheads indicate cortical LET-99. The scale bar represents 10 um. LET-99 localizes to the cortex, the polar body, metaphase chromosomes, and the spindle midzone. Plots display cortical fluorescence intensity versus embryo length. The anterior is to the left, and the posterior is to the right.(B and C) Spindle displacement assay.

Picture from Dong X et al. (2013) Cell "An extracellular adhesion molecule complex patterns dendritic branching and ...."

(G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells.

Picture from Vazquez-Manrique RP et al. (2007) Genomics "The frataxin-encoding operon of Caenorhabditis elegans shows complex ...."

(g-h) vrk-1::gfp was expressed in several cells, which included head neurons (g) and phasmids (h).

Picture from Fisher J et al. (2007) PLoS Comput Biol "Predictive modeling of signaling crosstalk during C. elegans vulval ...."

As examples for the time-course analysis, a mid-L2 larva at +22 h (A') and a late L2 larva at +28 h (B') are shown.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(H) Pgrl-27::GFP is expressed in arcade cells.

Picture from Yu S et al. (1997) Proc Natl Acad Sci U S A "Guanylyl cyclase expression in specific sensory neurons: a new family of ...."

(H 1-3) Expression pattern of gcy-32::GFP.

Picture from Fisher J et al. (2007) PLoS Comput Biol "Predictive modeling of signaling crosstalk during C. elegans vulval ...."

As examples for the time-course analysis, a mid-L2 larva at +22 h (A'') and a late L2 larva at +28 h (B'') are shown.

Picture from Cuppen E et al. (2003) Comp Funct Genomics "Proteins interacting with Caenorhabditis elegans Galpha subunits."

Expression pattern of T22A3.3 (H) as determined by promoter-GFP reporter constructs. (H) Expression of T22A3.3 is seen in a set of neurons in the head, including AWB.

Picture from Tepper RG et al. (2013) Cell "PQM-1 complements DAF-16 as a key transcriptional regulator of DAF-2-mediated ...."

(F-H) DAF-16 becomes more cytoplasmic with age, and nuclear localization of PQM-1::GFP decreases significantly with age. Data are represented as mean +/- SEM (G and H).