8 Pictures found (0.046 seconds)

Picture from Ezechukwu, Chiemekam Samuel et al. (2022) MicroPubl Biol

Figure 1. Cytological and phenotypic characterization of cosa-1:mCherry(wsh7):(A) Fluorescence images showing the localization of the COSA-1::mCherry foci in the different zones of the germline. White rings indicate examples of 6 COSA-1::mCherry foci per nuclei in the late pachytene (LP) of adult worms, blue arrows define germline distal to proximal direction (MZ - mitotic zone, TZ - transition zone, EP - early pachytene, MP - mid pachytene, LP - late pachytene, DP -diplotene). (B) Colocalization of GFP::COSA-1 and COSA-1::mCherry. (C - E) Analysis of brood size (C), progeny viability (D) and male frequency (E) in the cosa-1::mCherry(wsh7) transgenic strain compared with the wild type (ns - not significant; Independent samples t-test, n= 26).

Picture from Lee KK et al. (2000) Mol Biol Cell "C. elegans nuclear envelope proteins emerin, MAN1, lamin, and nucleoporins ...."

(B) C. elegans embryos triple stained for DNA (Hoechst 33258, left) and by indirect immunofluorescence with the use of antibodies against Ce-lamin (green) and tubulin (red; right; imaged by confocal microscopy). The stages of mitosis were determined by chromosome morphology and corroborated by tubulin staining patterns: I, interphase; PMP; prometaphase; LP, late prophase; EP, early prophase; M, metaphase.

Picture from Martin E et al. (2014) PLoS One "pix-1 controls early elongation in parallel with mel-11 and let-502 in ...."

Picture from Lee KK et al. (2002) Mol Biol Cell "Lamin-dependent localization of UNC-84, a protein required for nuclear ...."

Figure 6. UNC-84 localization during mitosis. Wild-type embryos (100-300 cells) were stained with rat anti-UNC-84 antibodies (right panels) and Hoechst 33258 to stain DNA (left panels), viewed by fluorescence microscopy and photographed at different stages of the cell cycle. A representative nucleus from each stage is shown: IP, interphase; EP, early prophase; LP, late prophase; PM, prometaphase; MT, metaphase; EA, early anaphase; LA, late anaphase and TL, telophase. Nuclear staining was detected in all stages of mitosis except late anaphase, consistent with other nuclear membrane markers (Lee et al., 2000).

Picture from Lee KK et al. (2000) Mol Biol Cell "C. elegans nuclear envelope proteins emerin, MAN1, lamin, and nucleoporins ...."

Figure 4. Persistence of nuclear envelope markers during mitosis in C. elegans. (A) Indirect immunofluorescence of endogenous Ce-emerin in a C. elegans embryo. DNA was stained with Hoechst 33258 (left), and the same embryo was stained for endogenous Ce-emerin with the use of mouse polyclonal serum 3272 (right; imaged by confocal microscopy). Arrows point to nuclei in prophase (P) and metaphase (M). (B) C. elegans embryos triple stained for DNA (Hoechst 33258, left) and by indirect immunofluorescence with the use of antibodies against Ce-lamin (green) and tubulin (red; right; imaged by confocal microscopy). The stages of mitosis were determined by chromosome morphology and corroborated by tubulin staining patterns: I, interphase; PMP; prometaphase; LP, late prophase; EP, early prophase; M, metaphase.

Picture from Thomas JH et al. (1999) Development "The C. elegans gene lin-36 acts cell autonomously in the lin-35 Rb ...."

Figure 4. lin-36::GFP reporter expression. Anterior is to the left; dorsal is up. (A,C) Photomicrographs taken using Nomarski optics. (B,D) Fluorescent images of the animals seen in A and C observed using an LP-FITC filter. Animals are from the rescued Roller line 3L26a, which carries an integrated reporter transgene and a co-integrated marker transgene. (A,B) L3 hermaphrodite with reporter expression in the nuclei of the P(3-8).p cells. The nuclei of ventral cord neurons adjacent to the P(3-8).p cells also expressed the reporter construct. (C,D) L3 hermaphrodites showing the nuclear expression of the reporter in the descendants of the P(3-8).p cells during vulval cell divisions. The lineage relationships of these cells are shown by lineage diagrams superimposed on the images. Bar, 20µm.

Picture from Martin E et al. (2014) PLoS One "pix-1 controls early elongation in parallel with mel-11 and let-502 in ...."

Figure 5. PIX-1::GFP is differentially expressed in hypodermal cells during elongation. A) Confocal lateral projections of pix-1(gk416);sajEx1[pix-1p::pix-1::GFP]; mcIs40 [lin-26p::ABDvab-10::mcherry + myo-2p::gfp] embryos. Dorsal-anterior (DA, upper panel), dorsal-posterior (DP, upper panel), ventral-anterior (VA, upper panel), ventral-posterior (VP, upper panel), lateral-anterior (LA, lower panel) and lateral-posterior (LP, lower panel) hypodermis are surrounded by dashed line and have been identified using lin-26p::vab-10(ABD)::MCHERRY hypodermal markers. B) Distributions ofthe dorsal-posterior/dorsal-anterior (DP/DA), lateral-posterior/lateral-anterior (LP/LA), ventral-posterior/ventral-anterior (VP/VA), dorsal-posterior/ventral (DP/V); dorsal-anterior/ventral (DA/V), dorsal-anterior/lateral (DA/L) rates of fluorescence intensity measured in pix-1(gk416); sajEx1[pix-1p::pix1::GFP; rol-6]; mcIs40 [lin-26p::ABDvab-10::mcherry + myo-2p::gfp] embryos between comma and 1.75-fold stages (n = 26 embryos). Similar results were also obtained in pix-1(gk416); sajIs1[pix-1p::pix-1::GFP; unc-119R]; mcIs40 [lin-26p::ABDvab-10::mcherry + myo-2p::gfp]. The box-plots represent the min, max, 25th, 50th (median) and 75th percentiles of the populations. ** T-test comparing ratios to 1 p,0.01. C) Schematic representation of pix-1::GFP and ABDVAB-10 (control) constructs used to measure the DP/DA intensity ratio reported in panel D. DP/DA of animals carrying mcIs40 (lin-26p::ABD::mCh expressing), mcIs50 (lin-26p::ABD::GFP expressing), sajIs2 (lin-26p::pix-1::GFP expressing) or sajEx1 (pix-1p::pix-1::GFP expressing). ratios** T-test comparing DP/DA ratios measured on pix-1::GFP expressing embryos to ratio measured in ABDVAB-10 expressing transgenics, p,0.01. The box-plotsrepresent the min, max, 25th, 50th (median) and 75th percentiles of populations.

Picture from Li Q et al. (2018) PLoS Genet "The tumor suppressor BRCA1-BARD1 complex localizes to the synaptonemal complex ...."

Fig 2. GFP::BRC-1 and BRD-1::GFP localize to the SC in meiotic prophase. A) Nuclei from indicated meiotic stages stained withRAD-51 antibodies (red), DAPI (blue) and imaged for GFP fluorescence (green). White arrows demark foci positive for both GFP fluorescence and RAD-51 signal; green arrows demark foci containing GFP but not RAD-51; red arrows demark foci containing onlyRAD-51. Scale bar = 5 um. B) Co-localization between GFP::BRC-1 (green) and SC central component SYP-1 (red) by antibody staining;germ lines at indicated stages were counterstained with DAPI. Blue arrows at late pachytene show chromosomal regions where GFP::BRC-1 concentrates before SYP-1. Scale bar = 2 um. C) TagRFP-T::BRC-1 (red) and GFP::COSA-1 (green) at late pachytene showingTagRFP-T::BRC-1 on one side of the GFP::COSA-1 focus, which marks the persumptive crossover. Scale bar = 2 um. Images areprojections through half of the gonad. TZ = transition zone; EP = early pachytene; MP = mid pachytene; LP = late pachytene;DP = diplotene; DK = diakinesis.