Picture from Brandt JP et al. (2015) Curr Biol "Toll-like Receptor Signaling Promotes Development and Function of Sensory ...."

Supplemental Figure 5, related to Figure 3. The TAK-like kinase MOM-4 and the p38 MAPK PMK-3 are expressed by BAG neurons. A. Fluorescence micrographs of animals carrying a fosmid-based mom-4::GFP translational reporter and the BAG cell-specific reporter, Pflp-17::dsRed. B. Fluorescence micrographs of animals carrying a fosmid-based pmk-3::GFP translational reporter and the BAG cell-specific reporter Pflp-17::dsRed. Scale bar represents 10 um.

Picture from Brandt JP et al. (2015) Curr Biol "Toll-like Receptor Signaling Promotes Development and Function of Sensory ...."

Supplemental Figure 4, related to Figure 3. A transcriptional reporter for tol-1 is expressed in sensory neurons in the head. A. A representative micrograph of animals bearing a Ptol-1::GFP transgene. White arrows indicate URY sensory neurons, which strongly express the reporter. Because of the intense expression of the reporter in URY neurons, the expression of the reporter in BAG neurons cannot be seen in this micrograph. Scale bar represents 10 um.

Picture from Brandt JP et al. (2015) Curr Biol "Toll-like Receptor Signaling Promotes Development and Function of Sensory ...."

(C) Fluorescence micrograph showing a single optical section of an animal carrying a Ptol-1::GFP transcriptional reporter and the BAG cell-specificreporter Pflp-17::dsRed. This section did not capture strong GFP expression in URY neurons (see Figure S3).

Picture from Brandt JP et al. (2015) Curr Biol "Toll-like Receptor Signaling Promotes Development and Function of Sensory ...."

Figure 3. The TAK Homolog MOM-4 and the TLR TOL-1 Are Required for the Function of CO2-Sensing BAG Neurons (A) CO2-avoidance indices of the wild-type, dlk1(ju475), sek-1(km4), mom-4(gk563), and mom4(gk563) mutants carrying a transgene for BAG-specific expression of mom-4.(B) CO2-avoidance assays of the wild-type, tol1(nr2033), and tol-1(nr2013) mutants, animals carrying a transgene for BAG cell-specific RNAi of tol-1, and tol-1(nr2013) mutants carrying a transgene for BAG cell-specific expression of tol-1. (C) Fluorescence micrograph showing a single optical section of an animal carrying a Ptol-1::GFP transcriptional reporter and the BAG cell-specificreporter Pflp-17::dsRed. This section did not capture strong GFP expression in URY neurons (see Figure S3). (D) Expression of transgene markers of differentiated BAG neurons in the wild-type and animals carrying a transgene for BAG cell-specific RNAi of tol-1, normalized to wild-type levels, which are replotted from Figure 1C. Promoters from the indicated genes were used to drive expression of either GFP or mCherry. For these bar graphs, data are plotted as means +- SEM. n R 20 for all measurements. Asterisks indicate statistical significance at p % 0.05 for the indicated comparisons. Error bars represent SEM.