Picture from Ghosh-Roy A et al. (2012) Dev Cell "Kinesin-13 and tubulin posttranslational modifications regulate microtubule ...."

(C) Quantitation of cell body fluorescence intensity in anterior neurons, normalized to wild type. n=0 animals per genotype; statistics, Mann-Whitney test

Picture from Ryan DA et al. (2014) Curr Biol "Sex, age, and hunger regulate behavioral prioritization through dynamic ...."

(c) Quantitation of Podr-10::GFP (kyIs37) expression. Fluorescence intensity was scored qualitatively using four categories (absent, faint, moderate and bright) with investigator blinded to genotype. Comparison of hermaphrodites (n = 24) vs. males (n = 28) was done by Mann-Whitney test; ***p < 10-5.

Picture from Leung AC et al. (2020) MicroPubl Biol "Lipopolysaccharide stimulates egg laying in Caenorhabditis elegans"

Wild-type N2 strain of C. elegans was stimulated by Salmonella enterica lipopolysaccharide (LPS) to lay eggs in an aqueous environment: Animals were incubated in M9 buffer for 15 minutes, after which the numbers of eggs laid were recorded for adjustment purpose. The liquid medium was then supplemented with either LPS or serotonin, or with no supplement as the negative control. The final concentration of LPS was 0.1 mg/ml and that of serotonin was 1 mg/ml. After one hour, the eggs in each microtiter well were counted again. For each individual, the adjusted number of eggs laid, which considered only the eggs laid during the treatment period, is presented here. More than 100 animals under each condition were tested. Error bars represent standard errors; asterisks denotes p < 0.01 when the treatment condition (LPS or serotonin) resulted in an egg-laying level that was significantly different from that of the negative control condition (M9), when the non-parametric Mann-Whitney U Test (Mann and Whitney 1947) was performed. The horizontal bar and the asterisks above it indicate that there was a significant difference (p < 0.01, Mann-Whitney U Test; Mann and Whitney 1947) between the numbers of eggs laid under the LPS and serotonin conditions.

Picture from Zilberman Y et al. (2017) J Cell Biol "Cdc42 regulates junctional actin but not cell polarization in the ...."

(B) Quantification of colocalization at the junctions for HMR-1/ PAC-1 (n = 9), DLG-1/ PAC-1 (n = 9), and PAC-1/ PICC-1 (n = 8) using the Pearsoncorrelation coefficient (PCC) test. Red bars and error bars are mean +- SD. P-values were calculated using a Mann-Whitney U test. *, P < 0.05. Bars, 5 um.

Picture from Shinkai Y et al. (2018) MicroPubl Biol "EOR-1 mediates non-cell autonomous regulation of abts-1 gene expression in ...."

Figure 1. Non-cell autonomous regulation of abts-1b expression in HSNs.(A) A representative image of an HSN at L3 larval stage, obtained from strain CX5974. The closed arrowhead indicates the cell body of an HSN and the open arrowhead indicates an elongating neurite from the cell body. Arrows indicate the axon of PLM neuron. White scale bar indicates 10 um.(B) Comparison of Pabts-1b::gfp expression in HSNs between wild-type and deg-3(u662) mutant worms at the adult stage. Error bars indicate SEM (n = 49, **p < 0.01, Mann-Whitney U test).(C) Pabts-1b::gfp expression in HSNs was analyzed in worms expressing PKC-1(gf) under the control of the mec-7 promoter. Data from wild-type or eor-1(cs28) mutant worms are shown. Worms carrying the transgene (+Ex) and worms not carrying the transgene (Ex) were compared. Error bars indicate SEM (n = 22, **p < 0.01, Mann-Whitney U test).

Picture from Zilberman Y et al. (2017) J Cell Biol "Cdc42 regulates junctional actin but not cell polarization in the ...."

Figure S4. Colocalization of PAC-1 with HMR-1 or DLG-1. Related to Fig. 3. (A) Merge of mCherry-PAC-1 and DLG-1 staining of the embryo shown inFig. 3 B. (B) Quantification of colocalization at the junctions for HMR-1/ PAC-1 (n = 9), DLG-1/ PAC-1 (n = 9), and PAC-1/ PICC-1 (n = 8) using the Pearsoncorrelation coefficient (PCC) test. Red bars and error bars are mean +- SD. P-values were calculated using a Mann-Whitney U test. *, P < 0.05. Bars, 5 um.

Picture from Rappaport, Yisrael et al. (2022) MicroPubl Biol

Figure 1 Fertility analyses of linc-9; linc-20:A-C. Box plots depict the average number (x), the median (horizontal line within the box) and the individual measurements (circles) of brood size per worm. D-F. Box plots depict the average number (x), the median (horizontal line within the box) and the individual measurements (circles) of embryonic lethality level per worm. Data for A, B, D, E was adapted from (Ishtayeh et al., 2021). N values appear above each box plot. Mann-Whitney p value: NS - not significant.

Picture from Silva M et al. (2017) Curr Biol "Cell-Specific -Tubulin Isotype Regulates Ciliary Microtubule ...."

C TBA-6::GFP transgene from Hurd et al. 2010 [S1] can partially rescue the curved-in CEM cilia phenotype in tba-6 CEM cilia (refer Figure 2). TBA-6::GFP expression in wild type and tba-6 mutant backgrounds. Brackets indicate the length of the CEM cilia. CEM cilia curvature in tba-6 animals expressing the TBA-6::GFP transgene is quantifiedon left: tba-6 curved-out cilia 80+-5, tba-6 curved-in cilia 19+-5, mean+-SEM, '***' indicate p-value <0.0001 via Mann Whitney test. N= 42 number of animals. Scale bar is 50 umRelated to Figure 2

Picture from Champion, Margaret et al. (2021) MicroPubl Biol

Figure 1. FIC-1 activity does not affect cellular ATP levels: (A) Schematic of fic AMPylase-mediated target modification. (B) ATP concentration measurements in lysates of wild-type (N2), fic-1 KO (n5823), FIC-1(E274G)-expressing (nIs733), and inducibly FIC-1(E274G) over-expressing (nIs774) worms. For (B): We used an ordinary one-way ANOVA test with multiple comparisons to calculate statistical significance. The comparison of uninduced and induced nIs774 was done using the Mann-Whitney test. Circles and squares within samples highlight different biological replicates. Each circle/square depicts a single-well value (technical replica). The total dataset consists of two biological replicates, with 8 technical replicates each.

Picture from Garcia-Gonzalez, Bianca et al. (2022) MicroPubl Biol

A: Temperature affects neuronal expression of GFP driven by the unc-33 promoter. Representative confocal micrographs of the head region of C. elegans nematodes grown on OP50 at either 16°C or 25°C are shown on the left. Quantification of the mean maximum fluorescence plotted as the ratio of fluorescence intensity in neurons over glial cells is shown on the right. Plotted is the mean ± SD, n=30 nematodes/strain/condition. The Mann-Whitney test for non-parametric samples denotes a statistically significant difference with a p-value <0.0001 (****). B: The neuronal expression of GFP driven by the unc-33 promoter is significantly changed by Temperature. Temperature tolerance curve was constructed by quantifying the ratio of fluorescence intensity in neurons over glial cells of nematodes grown at temperatures of 16°C to 25°C. Plotted is the mean ± SD, n=30 nematodes/strain. One way ANOVA with post hoc Tukey's honestly significant difference (HSD) test denotes statistically significant differences between groups labeled as "a" (16°C), "b" (17-20 °C) and "c" (22-25 °C), p-value <0.01 (**). C: Nematodes grown in different bacterial types showed a statistically significant difference in neuronal expression of GFP driven by the unc-33 promoter at 16°C. Quantification of the neuronal to glia fluorescence ratio of C. elegans incubated on plates seeded with E.coli OP50 or P. aeruginosa PA14 at 16°C. Plotted is the mean ± SD, n=20 nematodes/strain. The Mann-Whitney test for non-parametric samples denotes a statistically significant difference with a p-value <0.0001 (****). D: Nematodes grown in different bacterial types showed no significant difference in neuronal expression of GFP driven by the unc-33 promoter at 25°C. Quantification of the neuronal to glia fluorescence ratio of C. elegans incubated on plates seeded with E. coli OP50 or P. aeruginosa PA14 at 25°C. Plotted is the mean ± SD, n=24 nematodes/strain. The Mann-Whitney test for non-parametric samples denotes no significant difference (ns) between the two samples.