Figure 4. NPR-1 Is Expressed in Neurons. Confocal projection of an adult hermaphrodite showing
npr-1:GFP expression in neurons in the head (ventro-lateral view; anterior is top left). GFP expression is driven from construct pM6, which encodes C-terminally truncated NPR-1 tagged at its C terminus with GFP (See Figure 3A). The animal shown is
npr-1(
ad609) but is restored to solitary behavior by the rescuing pM6 construct. Expression is concentrated in the nerve ring, the major process bundle of the head (arrow). Several neuronal cell bodies are visible; since this staining is widespread and faint, it has not been characterized in detail. Neuronal processes of the ventral nerve cord (large arrowhead) and pharynx (small arrowhead) also express GFP.