Figure S4 MATH-33 and USP-47 are present in the germline. Micrographs showing oocytes and a mitotic portion of the gonad for each indicated genotype. (A) Worms immunostained for MATH-33 (red), DAPI (blue), and an overlay (red & blue). (B) Worms immunostained for USP-47 (red), DAPI (blue), and an overlay.
MATH-33 is present in the germline. Micrographs showing oocytes and a mitotic portion of the gonad. (A) Worms immunostained for MATH-33 (red), DAPI (blue), and an overlay (red & blue).
(B) Wild-type perinuclear germline localization of DRH-3,(left, yellow) (DNA, center, blue).(C) DRH-3 (left, green) colocalizes with the P granule component, PGL-1 (center, red; DNA, blue).(D) DRH-3 (left, yellow) remains localized to P granules in the embryonic P cell lineage (dashed circles; DNA, center, blue).
(A) Dissected L4 gonad stained with OEF-1 (green) and thespermatogenesis marker SP56 (red). DAPI is in blue. (B) Dissected adult gonad stained with OEF-1 (green) and the oogenesis marker OMA-1/2 (red).DAPI, blue.
EGO-1 Localizes to Chromosomes. (D) Single confocal sections of EGO-1 in wild-type embryo prophase/prometaphase (tubulin, green; DNA, blue).(H) Single confocal sections of EGO-1 in wild-type embryo metaphase (tubulin, green; DNA, blue).
DRH-3 Localizes to Chromosomes. (C) Single confocal sections of DRH-3 in wild-type embryo prophase/prometaphase (tubulin, green; DNA, blue).(G) Single confocal sections of DRH-3 in wild-type embryo metaphase (tubulin, green; DNA, blue).
EKL-1 Localizes to Chromosomes. (E) Single confocal sections of EKL-1 (E) (red) in wild-type embryo prophase/prometaphase (tubulin, green; DNA, blue).(I) Single confocal sections of EKL-1 (I) (red) in wild-type embryo metaphase (tubulin, green; DNA, blue).(J and K) Single confocal sections of EKL-1 in wild-type embryo early (J) and late (K) anaphase (tubulin, green; DNA, blue).