Picture from Chen WI et al. (1993) J Biol Chem "A new member of the ras superfamily, the rac1 homologue from Caenorhabditis ...."

FIG.3. Developmental regulation of the expression of the CErac1 mRNA. a, totalRNA was prepared from the various C. elegant developmental stages and samples (5pg) from embryos(E), larvae(L1 to L4), adults (A), and mature adults(MA)were subjected to Northern blot analysis as described under 'Experimental Procedures.' The autoradiogram was exposed a t -70 C for 3 days. b, the same filter was stripped in 50% formamide solution, then hybridized with a 32P-labeled cDNA probe for actin mRNA (18),and the auto- radiogram was exposed over night a t -70 C. c, signals from autoradiograms were quantified separately by scanning densitometry in a n Ultroscan XL laser densitometer. The data were normalized to the integrated intensities of signals obtained a t each developmental stage with a 32P-labeled cDNA probe for actin mRNA. The relative abundance of the 1.7-kb CEracI mRNA at each developmental stage is shown.

Picture from Banerjee D et al. (2005) Dev Cell "Developmental timing in C. elegans is regulated by kin-20 and tim-1, homologs of ...."

Figure 3. KIN-20 Is the Closest C. elegans Homolog to Drosophila DOUBLETIME. (A) Phylogenetic tree of casein kinase I-predicted protein sequences from the following metazoans: Hs, Homo sapiens; Ma, Mesocricetus auratus; Ce, Caenorhabditis elegans; Dm, Drosophila melanogaster.(B) Schematic of kin-20 isoforms. Solid bars represent translated sequences and hatched bars represent untraslated sequences.(C) mRNA expression profiles of kin-20. A developmental Northern blot with total RNA from each developmental stage (E, embryos; L1-L4, larval stages 1-4; YA, young adult) was probed with a kin-20 fragment. Three signals were detected at all developmental stages (kin-20C at 4.0 kb, kin-20A at 2.5 kb, and kin-20B at 1.8 kb).(D) Schematic of the kin-20::gfp translational fusion construct for kin-20A and kin-20C isoforms. The C-terminal GFP fusion consists of kin-20-translated exons 3-7 under control of 3 kb of kin-20 native promoter, and with the unc-54 3'UTR.(E and F) KIN-20::GFP is expressed in seam cells and the hermaphrodite-specific neuron (HSN). (E) KIN-20::GFP localization to dividing seam cell pairs (indicated by small white arrowheads) in an L4-stage animal (magnification of 630). (F) KIN-20::GFP localization to the HSN (indicated by the large white arrowhead) in a late L4-stage animal (magnification of 1000). In (E) and (F), anterior is oriented toward the left, and dorsal is oriented toward the top.