Picture from Zhu M et al. (2015) PLoS One "Serum- and Glucocorticoid-Inducible Kinase-1 (SGK-1) Plays a Role in Membrane ...."

Fig 1. Both putative sgk-1(null) alleles abolished the plasma membrane localization of MIG-14::GFP in intestinal cells. Confocal images of theintestine of wild type (A, A'), sgk-1(ok538) (B, B') and sgk-1(mg455) (C, C') animals expressing MIG-14::GFP. In wild type intestinal cells, MIG-14::GFP is seen on the basolateral membrane (arrows, best viewed on a focal plane near the top of the cell) but not on the apical membrane (arrowheads, best viewed on a focal plane in the middle of the cell). Scale bars: 5 um.

Picture from Zhu M et al. (2015) PLoS One "Serum- and Glucocorticoid-Inducible Kinase-1 (SGK-1) Plays a Role in Membrane ...."

Fig 1. Both putative sgk-1(null) alleles abolished the plasma membrane localization of MIG-14::GFP in intestinal cells. Confocal images of theintestine of wild type (A, A'), sgk-1(ok538) (B, B') and sgk-1(mg455) (C, C') animals expressing MIG-14::GFP. In wild type intestinal cells, MIG-14::GFP isseen on the basolateral membrane (arrows, best viewed on a focal plane near the top of the cell) but not on the apical membrane (arrowheads, best viewedon a focal plane in the middle of the cell). Scale bars: 5 um. (D) Quantitation of average pixel intensity of MIG-14::GFP localized in the cytoplasm. The average intensity is denoted with a red line. *** P value <0.001, ** P value <0.01 (Wilcoxon rank sum test).

Picture from Barbosa DJ et al. (2017) PLoS Genet "Dynactin binding to tyrosinated microtubules promotes centrosome centration ...."

(A), (B) Correlation plots of GFP::p50DNC-2versus EBP-2::mKate2 intensity (A) anddynein heavy chainDHC-1::GFP versus EBP-2::mKate2 intensity (B), measured at thecortex of metaphase one-cell embryos. Pearson correlation coefficient (r) and P-valueindicating statistical significance are on top right. The best-fit line of a linear regressionwith 95% confidence bands is also shown.

Picture from Cuenca AA et al. (2003) Development "Polarization of the C. elegans zygote proceeds via distinct establishment and ...."

Figure 3. GFP:MEX-5 and PIE-1:GFP dynamics in wild-type embryos (Movies 5 and 6). (A) Pronuclear formation. (B-D) Pronuclear migration. (D) Pseudocleavage. (F) Pronuclear meeting. (G) Mitosis. (H) Two-cell stage. Note that PIE-1:GFP accumulates in the male pronucleus during pronuclear migration, on the posterior centrosome during mitosis, and in the P1 nucleus in the two-cell stage. PIE-1:GFP and GFP:MEX-5 also accumulate on the cytoplasmic P granules (best seen in the movies).

Picture from Barbosa DJ et al. (2017) PLoS Genet "Dynactin binding to tyrosinated microtubules promotes centrosome centration ...."

S1 Fig. Dynein-dynactin levels and dynamics at the cell cortex.(A), (B) Correlation plots of GFP::p50DNC-2versus EBP-2::mKate2 intensity (A) anddynein heavy chainDHC-1::GFP versus EBP-2::mKate2 intensity (B), measured at thecortex of metaphase one-cell embryos. Pearson correlation coefficient (r) and P-valueindicating statistical significance are on top right. The best-fit line of a linear regressionwith 95% confidence bands is also shown.(C), (D) Residency times of EBP-2::mKate2 (C) and GFP::p50DNC-2(D) puncta at thecortex of metaphase one-cell embryos. The total number (n) of MT plus ends scored isindicated, derived from at least 8 embryos.

Picture from Hasnain, Pirzada et al. MicroPubl Biol

Figure 1. Phylogenetic annotation of Caenorhabditis elegans 70 kDa heat shock protein (Hsp70) genes:(A) Bayesian phylogenetic tree constructed using the LG + I + G model (M-Coffee alignment, best model according to BIC). Deduced amino acid sequences were used, and the mitochondrial lineage was set as the root. Numbers indicate the posterior probability support for each node. Saccharomyces cerevisiae SSA1 (NP_009396.2), SSA2 (NP_013076.1), SSA3 (NP_009478.1), SSA4 (NP_011029.3), KAR2 (NP_012500.3), and SSC1 (NP_012579.1); Coral Acropora millepora HSP70cA1 (XP_029210797.1), HSP70cB1 (XP_029206535.1), HSP70cB2 (XP_029194855.1), HSP70erA1 (XP_029187601.1), HSP70mA1 (XP_029208733.1); Homo sapiens HSPA1A/B (NP_005337.2), HSPA1L (NP_005518.3), HSPA2 (NP_068814.2), HSPA5 (NP_005338.1), HSPA6 (NP_002146.2), and HSPA9 (NP_004125.3). (B, C) M-coffee alignment of C. elegans (Ce) and A. millepora (Am) Hsp70s. Residues characteristic to the cytosolic, endoplasmic reticulum, and mitochondrial lineages are shown in blue, green, and red, respectively. The HSP70cA-specific serine residues are highlighted. The potential target of gene conversion is indicated by a black triangle. Numbers on the right of each panel indicate amino acid numbers from the N-terminus.

Picture from Strome S et al. (2001) Mol Biol Cell "Spindle dynamics and the role of gamma-tubulin in early Caenorhabditis elegans ...."

Figure 5. Multiphoton image series of GFP::beta-tubulin in a live wild-type embryo. Elapsed time from the first frame is shown in minutes:seconds. Brightness and contrast were adjusted to better reveal single microtubules in the top row and centrosome dynamics in the bottom 3 rows. Microtubule dynamics can be seen best in Movies 2 and 3. (A-C) Metaphase and early anaphase. (D-L) Anaphase B and telophase. The posterior spindle pole underwent dramatic side-to-side swinging during spindle elongation (C-G). Many astral microtubules extended to near the cortex. They usually appeared 'swept back' on the side from which the spindle pole was moving (D). Some microtubules bent on the side to which the spindle pole was moving (see Movies 2 and 3). Note the bright area at the equator (D-F), which likely represents the region of interdigitation of interpolar microtubules. (G-L) Telophase and cytokinesis. The dark centers of the centrosomes enlarged, and the posterior mother centrosome flattened and fragmented. Two daughter centrosomes appeared within each dark center.

Picture from Loges, Luiza N. et al. (2021) MicroPubl Biol

Figure 1. The D60N and R252L mutations in GSPD-1 reduce enzyme activity, and the R252L mutation appears to alter the position of the active site residues: (A) The protein sequence alignments of C. elegans GSPD-1 (top line) and human G6PD (bottom line) surrounding the two mutation sites (underlined) are shown. The middle line of text shows identical amino acids, and a + symbol indicates a conservative substitution. The numbers show the amino acid positions at the ends of each sequence. (B) Michaelis-Menten graph of enzyme velocities (in μmol/min/mg or U/mg) for wild-type GSPD-1 (blue circles), the D60N mutant (green triangles), and the R252L mutant (purple squares) at varying glucose-6-phosphate (G6P) concentrations. All of the data points are shown, and the lines indicate the best-fit line. (C) This table shows the results from the Michaelis-Menten fits shown in B with standard errors. (D) View of the active site in GSPD-1 models. The wild-type structure is shown in beige, and the R252L structure is shown in light blue. Asp264 and His269 bind to the glucose moiety of G6P, His207 and Tyr208 bind to the phosphate moiety of G6P, and Arg252/Leu252 are at the site of the mutation. The positions of these amino acids in each structural model are shown.

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Adulthood, in which the full pattern can best be seen, has 15 neurones staining in the head and tail ganglia, with a further 5 (clearly seen in this image) in the most anterior region of the ventral nerve cord