Figure 1 (A)
kqt-3 is 8869bp long and encodes KQT-3, a voltage-gated potassium channel. The
tm542 deletion removes 1001bp beginning in exon 2 and ending within intron 4. The
av174 deletion removes 9004bp beginning 69bp upstream of the ATG and ending 66bp after the stop (
av174). (B-D) The full length deletion of
kqt-3 does not mimic the smaller deletion allele, suggesting defects in (B) pump frequency, (C) duration, and (D) inter-pump interval are not due to
kqt-3 mutations.
kqt-3(
tm542) mutants differ greatly from WT in all three measures (one way ANOVA, Tukey's multiple comparisons test, p<0.001), but
kqt-3(
av174) mutants do not (one way ANOVA, Tukey's multiple comparisons test, ns). Creation of the full-length deletion in the
kqt-3(
tm542) background, notated
kqt-3(
tm542av187), does not rescue the pumping defects observed in the
tm542 mutant. Introns not to scale. Bars represent mean 1 SD. Average n=53.