Picture from Basham SE et al. (2001) Development "The Caenorhabditis elegans polarity gene ooc-5 encodes a Torsin-related ...."

Figure 4. Comparison of OOC-5, OOC-3 and HDEL staining in wild-type embryos. Confocal micrographs of wild-type embryos double labeled with anti-OOC-5 (A,D,G,J) or anti-OOC-3 (M,P,S) and anti-HDEL antibodies (B,E,H,K,N,Q,T). Merged images showing the extent of signal overlap (yellow) are shown in C,F,I,L,O,R,U. (A-L) Co-localization of OOC-5 and HDEL. (A-C) One-cell prophase embryo with one pronucleus visible; the other pronucleus is out of the plane of focus. (D-F) One-cell embryo in metaphase. (G-I) Two-cell in which AB (left cell) is in anaphase and P1 is in metaphase. (J-L) Four-cell embryo in which the AB cells (top left cells) are in prophase and EMS and P2 are in interphase. (M-U) Co-localization of OOC-3 and HDEL staining. (M-O) One-cell embryo in anaphase. (P-R) Two-cell embryo in early interphase. (S-U) Two-cell embryo in which AB is in anaphase and P1 is in metaphase. The stage of the cell cycle was determined by DAPI staining (not shown) in all cases. Scale bar: 10 um.

Picture from Basham SE et al. (2001) Development "The Caenorhabditis elegans polarity gene ooc-5 encodes a Torsin-related ...."

Distribution of OOC-5 in embryos. Confocal micrographs of wild-type embryos (A-O) double labeled with anti-OOC-5 (A,D,G,J,M) and alpha-tubulin antibodies (B,E,H,K,N); merged images are shown in C,F,I,L,O, where the overlap in signal appears yellow. (A-C) One-cell embryo at prophase; (D-F) one-cell anaphase; (G-I) two-cell interphase; (J-L) two-cell embryo in which the AB cell (left) is in anaphase and P1 is in metaphase. (M-O) Four-cell embryo in which the AB cells are dividing (top left cells, spindle poles only visible), EMS is in prophase and P2 (right most cell) is in interphase. The stage of the cell cycle was determined by DAPI staining (not shown) in addition to microtubule staining for all embryos. Posterior is towards the right in this and all figures. Scale bar: 10 um.

Picture from Basham SE et al. (2001) Development "The Caenorhabditis elegans polarity gene ooc-5 encodes a Torsin-related ...."

Figure 3. Distribution of OOC-5 in embryos. Confocal micrographs of wild-type embryos (A-O) double labeled with anti-OOC-5 (A,D,G,J,M) and alpha-tubulin antibodies (B,E,H,K,N); merged images are shown in C,F,I,L,O, where the overlap in signal appears yellow. Confocal images of ooc-5(it145) mutant embryos double labeled with anti-OOC-5 (P,R) and alpha-tubulin (Q,S) antibodies. (A-C) One-cell embryo at prophase; (D-F) one-cell anaphase; (G-I) two-cell interphase; (J-L) two-cell embryo in which the AB cell (left) is in anaphase and P1 is in metaphase. (M-O) Four-cell embryo in which the AB cells are dividing (top left cells, spindle poles only visible), EMS is in prophase and P2 (right most cell) is in interphase. (P-Q) One-cell anaphase ooc-5 mutant embryo. (R,S) Two-cell interphase ooc-5 mutant embryo. The stage of the cell cycle was determined by DAPI staining (not shown) in addition to microtubule staining for all embryos. Posterior is towards the right in this and all figures. Scale bar: 10 um.

Picture from Basham SE et al. (2001) Development "The Caenorhabditis elegans polarity gene ooc-5 encodes a Torsin-related ...."

Confocal micrographs of wild-type embryos double labeled with anti-OOC-5 (A,D,G,J) and anti-HDEL antibodies (B,E,H,K,N,Q,T). Merged images showing the extent of signal overlap (yellow) are shown in C,F,I,L,O,R,U. (A-L) Co-localization of OOC-5 and HDEL. (A-C) One-cell prophase embryo with one pronucleus visible; the other pronucleus is out of the plane of focus. (D-F) One-cell embryo in metaphase. (G-I) Two-cell in which AB (left cell) is in anaphase and P1 is in metaphase. (J-L) Four-cell embryo in which the AB cells (top left cells) are in prophase and EMS and P2 are in interphase. The stage of the cell cycle was determined by DAPI staining (not shown) in all cases. Scale bar: 10 um.

Picture from Horn M et al. (2014) Dev Cell "DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans ...."

(A) Representative DIC and fluorescence images showing overlapping expression patterns of DRE-1-, BLMP-1- and NHR-25::GFP in seam (se) and hypodermal (hyp) nuclei.

Picture from Fielenbach N et al. (2007) Dev Cell "DRE-1: an evolutionarily conserved F box protein that regulates C. elegans ...."

Figure 4. dre-1::gfp Expression Pattern. (A) Seam (se) and hypodermal (hyp) cells (mid-L3).(B) Seam (se) and hypodermal (hyp) cells (adult).(C) Vulval cells (vpc, all cells aligned in a row are vulva precursor cells) and anchor cell (ac) (mid-L3).(D) Distal tip cells (dtcs), body wall muscle cell (mu), and CAN neuron (around the L3m) (dhEx346).(E) Epidermal cell (epi) embryo.Nomarski images are shown beneath each gfp image. (A)-(C) and (E) show the dhEx453 gfp transgene; (D) shows the dhEx346 gfp transgene. The scale bars are 10 um.

Picture from Yin X et al. (2004) Mol Biol Cell "Inositol 1,4,5-trisphosphate signaling regulates rhythmic contractile ...."

Figure 6. Effect of dsRNA-induced disruption of plc-1 and plc-3 expression on sheath contractility. (A) Rates of basal sheath contraction in adult worms fed GFP, plc-1, or plc-3 dsRNA beginning at the L1 larval stage. Values are means ± SE (n = 7-29). *p < 0.01 compared with GFP dsRNA-fed worms. (B) Rates of basal and peak ovulatory sheath contractions in adult worms fed dsRNA for 16-24 h beginning in late-stage L4 larvae. Values are means ± SE (n = 8-13). *p < 0.05 and **p < 0.01 compared with GFP dsRNA-fed worms. (C) PLC-3::GFP expression pattern. Confocal differential interference contrast micrograph (left) and corresponding GFP fluorescence micrograph (right). GFP is expressed in proximal gonad sheath cells (arrowheads), spermatheca (Sp) and uterine sheath cells (arrow). DG, distal gonad; Int, intestine; Oo, oocyte.

Picture from Li W et al. (2015) J Cell Biol "Somatic CRISPR-Cas9-induced mutations reveal roles of embryonically ...."

Figure 5. IFT of LIS-1, IFT velocities, and the proposed model. (A) Colocalization of GFP:: LIS-1 with OSM-6::mCherry (red) in cilia (left), and a kymograph of GFP::LIS-1 motility (right). Micrograph bar, 5 um; kymograph horizontal bar, 2 um; vertical bar, 5 s. (B) Velocities of IFT particle and dynein subunits (mean +- SE). (C) A proposed model of subunits in the IFT-dynein complex and multiple turnaround sites on the cilium.

Picture from Li W et al. (2015) J Cell Biol "Somatic CRISPR-Cas9-induced mutations reveal roles of embryonically ...."

Figure 5. IFT of LIS-1, IFT velocities, and the proposed model. (A) Colocalization of GFP:: LIS-1 with OSM-6::mCherry (red) in cilia (left), and a kymograph of GFP::LIS-1 motility (right). Micrograph bar, 5 um; kymograph horizontal bar, 2 um; vertical bar, 5 s. (B) Velocities of IFT particle and dynein subunits (mean +- SE). (C) A proposed model of subunits in the IFT-dynein complex and multiple turnaround sites on the cilium.

Picture from Dent JA et al. (1998) Mech Dev "Post-embryonic expression pattern of C. elegans let-60 ras reporter ...."

Fig. 4. Miscellaneous staining. (A) Ventral view of young adult at mid-body. Arrowheads indicates ventral cord neurons. (B) Lateral view of young adult hypoderm. 'se' indicates seam cells from the V lineage. The other stained cells are syncytial hypoderm derived from the V lineage. (C) Lateral view of early L1 larva. 'i' indicates intestinal nuclei. (D) Lateral view of the adult pharynx. Pharyngeal muscle (pm) cells all stain. Nuclei surrounding the isthmus of the pharynx belong to neurons of the ring ganglion.