Picture from Barrios L et al. (1989) J Mol Biol "Genomic organization of the glyceraldehyde-3-phosphate dehydrogenase gene ...."

Figure 8. Ontogeny of mRNAs for gpd-4 and gpd-2. 10 g of total embryonic (E) and postembryonic larval (L4) mRNAs were dot blotted on to nitrocellulose paper and probed with gpd-2 and gpd-4-specific probes. The probe for gpd-2 was prepared using pSS1.3 (Fig. 3) derived from an M13 phage DNA using an oligonucleotide primer located 196 bp from its termination codon, which extended to a HaeIII site 5 bp before its stop codon. Since the 5' end of gpd-3 obtains a trans-spliced leader sequence (see Results and Discussion) this probe is specific for gpd-2 only. The gene-specific probe for gpd-4 was prepared using pB9 (Fig. 1(b)), an oligonucleotide 164 bp downstream from its termination codon, which was then extended to a HaeIII site located 10 bp beyond its stop codon.

Picture from Barrios L et al. (1989) J Mol Biol "Genomic organization of the glyceraldehyde-3-phosphate dehydrogenase gene ...."

Figure 7. Ontogeny of mRNAs for gpd-3 and gpd-1. Total RNA from the designated embryonic (E), 1st larval (L1), 2nd larval (L2) and 4th larval (L4) stage were purified and separated on agarose as described in Materials and Methods. Poly(A)' RNA was purified from total embryo RNA by oligo(dT)-cellulose chromatography (E+). RNA molecular weight standard markers were obtained from Bethesda Research Laboratories. RNA (10 g) was applied to each lane. (a) Upon transfer to a nitrocellulose filter, the panel of RNAs were hybridized with a probe specific for gpd-3. The gene specific probe for gpd-3 was made by primer extension of pSB (Fig. 3) using an oligonucleotide located 240 bp downstream from its termination codon, which extended to a HaeIII site located 5 bp before its stop codon. (b) A duplicate filter was challenged with a probe specific for gpd-1. The gene-specific probe for gpd-1 was made by primer extension of plasmid pSE3' (Fig. 1(a)) using an oligonucleotide primer 180 bp downstream from its termination codon. The extension was extended to a Sau3A site located 10 bp beyond the termination codon.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(L) Pgrl-6::GFP is expressed in the PVT neuron.

Picture from Munro E et al. (2004) Dev Cell "Cortical flows powered by asymmetrical contraction transport PAR proteins to ...."

(J-L) Embryos fixed at metaphase and costained for NMY-2 and PAR-3.

Picture from Yin J et al. (2017) J Cell Biol "GOP-1 promotes apoptotic cell degradation by activating the small GTPase Rab2 in ...."

(J-L") Confocal fluorescence images in wild type coexpressing GFP::GOP-1and markers of different organelles including Cherry::RAB-5 (J-J", early endosome), Cherry::RAB-7 (K-K", late endosome), or NUC-1::Cherry (L-L", lysosome). White arrowheads, vesicles with overlapping GFP and Cherry fluorescence; pink arrowheads, GFP- and Cherry-positive vesicles that are in closeproximity; yellow arrowheads, GFP-positive and Cherry-negative puncta. Quantification is shown at right (mean +- SD). Bars: (A-I') 10 um; (J-L") 5 um.

Picture from Tian Y et al. (2010) Cell "C. elegans screen identifies autophagy genes specific to multicellular ...."

(G-L) The epg-4 reporter is expressed ubiquitously in embryos (H) and widely in larvae (I). EPG-4::GFP colocalizes with the ER marker cherry::TRAM-1 (J-L). (G) Nomarski image of the embryo in H.

Picture from Pujol N et al. (2001) J Biol Chem "The Caenorhabditis elegans unc-32 gene encodes alternative forms of a vacuolar ...."

In situ hybridization images on embryonic, larval, and adult worms with vha-6 (I-L) are shown. vha-6 is expressed only in the intestine, from the embryonic coma stage (I) through larvae (J and K) and adult (L).

Picture from Mahoney TR et al. (2008) Proc Natl Acad Sci U S A "Intestinal signaling to GABAergic neurons regulates a rhythmic behavior in ...."

(G'-L') aex-2 is expressed in the GABAergic neurons AVL and DVB as well as in enteric muscles. (G and I) AEX-2::mCherry is detected in the nerve ring (NR, arrow), ciliary sensory processes (CA, open arrow), nerve cord (I), and head mesodermal cell (HMC, arrowhead). (H) AEX-2::mCherry is expressed in the intestinal muscle (IM, arrow) and anal depressor (AD, arrowhead). (J'-L') mCherry expressed under the aex-2 promoter is detected in AVL (J-L, arrow) and DVB (J''-L'', arrow) GABAergic neurons. AEX-2::mCherry signal does not significantly overlap with GABAergic GFP in the ventral nerve cord (J'-L'). (Scale bar, 20 um.) See SI Text for transgenes and clones.

Picture from Yoshimura S et al. (2008) Development "mls-2 and vab-3 Control glia development, hlh-17/Olig expression and ...."

Expression pattern of ptr-10::myrRFP reporter transgene. (G-L) Merged fluorescence and DIC images (G,I,K) and fluorescence images (H,J,L) of an adult animal expressing ptr-10::myrRFP. Expression in the head (G,H), vulva (I,J) and rectum (K,L) is shown. Arrowheads in H indicate the expression in sheath and socket glia of IL, OL (blue), and OLQ (yellow) sensilla, as well as in CEPsh glia (white).

Picture from Liu ZC et al. (1995) Development "The Caenorhabditis elegans heterochronic gene pathway controls ...."

Wild-type larvae (L) and adult (A) hermaphrodites transformed with the col-19/lacZ fusion. Bar 100 μm.