Picture from Park BJ et al. (2001) Mol Biol Cell "Calreticulin, a calcium-binding molecular chaperone, is required for stress ...."

(A) Northern blot analysis with 32P-labeled crt-1 probe detects a 1.4-kb band at all developmental stages. Eggs (E); larval stages (L2-L4); adults (A).

Picture from Huang, Xun et al. MicroPubl Biol

Table 1. RAS-ERK pathway and the canonical WNT signaling are likely not involved in RMED/V cell specification.Punc-25GFP expression in RMED/V cells in mutations in RAS or WNT signaling pathway components.

Picture from Bowles, Adria K et al. (2019) MicroPubl Biol

Figure 1 fem-3(e2006) L4 hermaphrodites were placed on lawns of OP50 E. coli (n=37), wildtype (wt.) V. vulnificus (n=65), or tonB1 tonB2 double mutant V. vulnificus (n=71) and assessed every 24 hrs. for survival. A Log-rank test indicated that there was no significant difference between survival on the two V. vulnificus strains (p=0.6), while both V. vulnificus strains cause significant reductions in survival relative to growth on OP50 (p<0.001).

Picture from Forbes JG et al. (2010) J Mol Biol "Extensive and modular intrinsically disordered segments in C. elegans TTN-1 and ...."

Fig. 3. Immunofluorescence localization of TTN-1 in the sarcomere. Each panel of three (a-c, d-f, etc.) confocal images shows results of co-staining adult body wall muscle with the indicated pairs of antibodies. (a-c) Anti-TTN-1 C-terminus antibody EU143 localizes both between dense bodies and outside of dense bodies in the I-band. Both MHC B and twitchin had been shown previously to localize to the A-band except for the middle of the A-band. Note that co-staining of EU143 with either MHC B (a-c) or with twitchin (g-i) shows some overlap at the outer edge of the A-band (yellow in c and i). (j-l) Antibody 9/10 localizes in the same region as the EU143 antibody. (m) Drawing of a portion of body wall muscle indicating the organization of the myofilament lattice and antibody staining in this obliquely striated muscle. The drawing is oriented such that the edge on view of the obliquely striated I-band and A-band stacks have the same orientation as in the immunofluorescence images. Dense bodies are analogous to Z-disks in mammalian cross striated muscle. (n) Proposed model of TTN-1 orientation, maximal span and variable environment in the sarcomere. The TTN-1 molecules span from the dense bodies toward the A-band in an N-to-C orientation, with some reaching as far as the edge of the thick filaments. Such molecules are misregistered across the sarcomere (bottom three filaments), due to the tapered shape of the dense body. The molecules are also offset significantly between adjacent sarcomeres due to the lateral offset of the sarcomeres in this obliquely striated muscle (top two filaments). The scale bar represents 5 um.

Picture from Kostrouchova M et al. (2002) Proc Natl Acad Sci U S A "SKIP is an indispensable factor for Caenorhabditis elegans ...."

Figure 2. Expression of CeSKIP during C. elegans development. (A) Agarose gel electrophoresis of simultaneous PCR products for CeSKIP (upper band) and ama-1 (lower band). E, L1, L2, L3, L4, A, and Mix represent embryos, larvae in L1-L4 stage, adult, and mixed population of worms, respectively. (B) Densitometric quantification combined from two independent experiments and quantified on four PCRs. The values are expressed as relative units of a ratio CeSKIP/ama-1, the bars represent standard deviation.

Picture from Hsieh HH et al. (2012) PLoS Genet "Integrin PAT-2/CDC-42 signaling is required for muscle-mediated clearance of ...."

Figure S1 pat-2 expression is not detectable in embryonic apoptotic cells. (A) GFP, Annexin V::mRFP, merged GFP and mRFP, and DIC images of a wild-type early embryo co-expressing Ppat-2nls::gfp and Phspannexin V::mrfp transgenes after heat shock treatment. Annexin V::mRFP, which is secreted and clusters around the surface of apoptotic cells, was used to label apoptotic cells. Apoptotic cells are indicated by arrows. The scale bar represents 5 mm. (B) GFP, Annexin V::mRFP, merged GFP and mRFP, and DIC images of a wild-type late embryo co-expressing Ppat-2nls::gfp and Phspannexin V::mrfp transgenes after heat shock treatment. Apoptotic MSpppaaa cells are indicated by arrows. The scale bar represents 5 mm.

Picture from Miller DM et al. (1983) Cell "Differential localization of two myosins within nematode thick ...."

Figure 4. lmmunofluorescence Localization of Myosin lsoforms in Nematode Body Wall Muscle Cells. (A, B) Polarized light micrographs of body wall muscle cells, Indirect immunofluorescence Image of body wall muscle cells labeled with antibody 26.2 (C, D) and antibody 5-6 (E, F). (A, C, E) are low magnification images and (B, D, F) are high magnification images. Bars are 10 um. The double-headed arrow in (B) depicts the course of a single myosin thick filament in the A-band. Pointers in (8, D, F) denote the center of the A-band.

Picture from Ross JM et al. (2003) Dev Cell "Polycomb group regulation of Hox gene expression in C. elegans."

(D) In a wild-type L3 male, egl-5::gfp is expressed in anterior and posterior daughtersof V ray precursors R4-R6.

Picture from Kim KW et al. (2009) Dev Cell "Antagonism between GLD-2 binding partners controls gamete sex."

(G) Western blot probed with rabbit anti-RNP-8 (top) or anti-actin (bottom). anti-RNP-8 recognizes the RNP-8L protein as a major band at 65 kD. On a longer gel, this single band resolves into several bands, which likely represent posttranslational modifications. anti-RNP-8 cannot detect RNP-8S. Emb, embryos; L1-L4, first-fourth larval stages; A, adult; q784, rnp-8(q784) homozygotes; tm2435, rnp-8(tm2435) homozygotes. glp-1(q224ts) hermaphrodites raised at the permissive temperature (15 C) have a nearly normal germline (+GL), but those raised at the restrictive temperature (25 C) have essentially no germline (-GL).

Picture from Anderson C et al. (2012) PLoS Genet "SLI-1 Cbl inhibits the engulfment of apoptotic cells in C. elegans through a ...."

Expression pattern of SLI-1::GFP under control of the sli-1 promoter. SLI-1::GFP was expressed under the control of its own promoter in an extrachromosomal array. (A) Images of morphologically normal animals expressing the SLI-1::GFP transgene. i, iii and v show fluorescence images and ii, iv and vi show accompanying DIC images (i-iv, embryos; v-vi, L4 larvae). Arrowheads in i-iv indicate positions of unengulfed cell corpses. In image iii corpses are not visible. Arrowheads in v and vi indicate the DTC. Bar = 5 microns.