Figure S7. Colocalization analysis of MAK-2 and CEBP-1 with presynapticmarkersAll images are of synaptic regions of the dorsal processes of GABAergic motorneurons. For each panel, pixel values obtained from line scans of a confocalprojection (on left) are plotted on the right. Pearson's correlation coefficient r and the probability were calculated. (A) mCherry::MAK-2 (juEx1503) partly colocalizes with SNB-1::GFP (juIs1). r = 0.69, P < 0.0001. (B) In
rpm-1(lf) mutants mCherry::MAK-2 and SNB-1::GFP are aberrantly distributed but remain colocalized. (C) mCherry::MAK-2 does not colocalize with RPM-1::GFP (juIs77). r = -0.14, P = 0.054. (D) mCherry::CEBP-1 (juEx1735) colocalizes with SNB1::GFP. r = 0.88, P < 0.0001.(E) mCherry::CEBP-1 is excluded from areas of RPM-1::GFP. r = -0.67, P < 0.001. (F) mCherry::MAK-2 and CEBP-1::GFP (juEx2157) partly colocalize; r = 0.48, P < 0.0001. Scale, 10 um.