Picture from Aram, Reta et al. MicroPubl Biol

Figure 1: Overview of plasmids for mosSCI at ttTi5605-compatible sites using G418 (NeoR) (A) or unc-119(+) (B) selection cassettes flanked by loxP sites. Both plasmids are modified from pCFJ151 (ttTi5605-MCS) (Frokjaer-Jensen et al. 2008). Results of test injections (three different transgene constructs for each plasmid) are shown below the diagrams. The mosSCI 'direct injection protocol' was followed, using Phsp::peel-1 and three mCherry(+) co-injection markers to screen against extrachromosomal arrays as described (Frokjaer-Jensen et al. 2012)(www.wormbuilder.org). Transgenes in pALS297/NeoRor pALS186/unc-119(+) were used for mosSCI injections into strains ALS344 or EG6699, respectively (Table 1).