Picture from Hulsey-Vincent H et al. (2023) MicroPubl Biol "A Fiji protocol for analyzing puncta is a robust tool for measuring GLR-1::GFP ...."

Figure 1. Comparison of quantification of GLR-1:GFP puncta using Igor and Fiji software:A. Representative images of WT and daf-7 mutant animals expressing GLR-1::GFP under the control of the glr-1 promoter. B-D. Left, original quantification using IgorPro software; Center; analysis of the same data set using the Fiji macros. Error bars represent SEM. Right, ImageJ quantification displayed as swarm plots to show average intensity values for individual animals. B. Quantification of puncta intensity (peak-to-bead) per animal. C. Quantification of puncta width measured in microns as Full-width at Half-maximal (FWHM) fluorescence. D. Quantification of puncta density per 10 microns, by animal.

Picture from Hulsey-Vincent H et al. (2023) MicroPubl Biol "Comparison and agreement between two image analysis tools for quantifying ...."

Figure 1. Comparison of analyses of widefield and confocal images of GFP:SNB-1 using Igor and Fiji:A, B. Representative images of WT and fshr-1 mutant animals expressing GFP::SNB-1 imaged using widefield and confocal microscopy, respectively. C. Quantification of puncta intensity (peak-to-bead) per animal. Left, original quantification using IgorPro software; Center; analysis of the same data set using the Fiji macros (shown as bar graphs, with error bars representing SEM, left, and swarm plots, right); Right, quantification of images acquired using confocal microscopy by the Fiji macros displayed as swarm plots to show normalized intensity values for individual animals. Statistics for all plots are described in the text. D, E. Quantification of puncta width measured as Full-width Half-maximal (FWHM) fluorescence (D) and puncta per 10 𝜇m (E).