Picture from Joo HJ et al. (2009) Biochem J "Caenorhabditis elegans utilizes dauer pheromone biosynthesis to dispose of ...."

The expression profile of mRNA level measured by qRT-PCR of dhs-28 and daf-22 genes in each stage of N2 worms. Values represent means +/- S.D. from three independent measurements.

Picture from Shah, Pooja et al. MicroPubl Biol

Figure 1. Embryonic viability and brood size of double mutants.: A) Embryonic viability was determined at 20C for a minimum of 10 worms of each genotype. All data points are plotted and the means are indicated. B) Normalized brood size at 20C is indicated for each double mutant. For each worm, brood size was normalized to the mean value of matched wild type controls. A minimum of 10 worms were assayed for each genotype. All data points are plotted and the means are indicated. Data was formatted using Plots of Data (Postma and Goedhart, 2019).

Picture from Susoy, Vladislav et al. (2019) MicroPubl Biol

Figure 1. Psto3::wrmScarlet reporter is expressed in R4BL/R and R8BL/R ray neurons.

Picture from Coleman-Hulbert, Anna L. et al. MicroPubl Biol

Longevity under adult β-guanidinopropionic acid exposure: Survival curves for C. elegans strain N2, C. briggsae strain AF16, and C. tropicalis strain JU1373 exposed to β-guanidinopropionic acid at various concentrations starting on the first day of adulthood. Only the lifespan of AF16 at 300 mM (mean=26.2 days, p=0.0123) differed significantly from the control (mean=31.5 days for AF16; control means for N2=18.8 days and JU1373=25.1 days; statistical comparisons were made with a Cox proportional hazards mixed-model using the coxme v.2.2-5 package in R (Therneau 2012; Lucanic et al. 2017)).

Picture from Johnson, Christina K. et al. (2021) MicroPubl Biol

Figure 1. Plasmin enhances MEC-4(d) but not UNC-8(d) currents in a MEC-6-dependent manner: Estimation plots by unpaired t-Test analysis of currents recorded in oocytes not treated and treated with plasmin (Calin-Jageman and Cumming 2019). Individual data points, means and 95% confidence intervals are shown. In each graph, on the right, the black circle and the error bars indicate the difference between the means and its 95% confidence interval. A: Currents recorded at -100 mV from oocytes expressing MEC-4(d)+MEC-2+MEC-6 not treated (light red) and treated (dark red) with 10 μg/ml plasmin, as indicated. N = 15, each. The difference between the means was 4.68 (71% increase), the lower and upper 95% confidence levels were 2.45 and 6.83 respectively (38% and 104% change, respectively). B: Same as panel A for oocytes expressing UNC-8(d)+MEC-2+MEC-6 not treated (light blue) and treated (dark blue) with plasmin. N = 7 and 5. The difference between the means was 0.94 (6% increase), the lower and upper 95% confidence levels were -1.50 and 3.38 respectively (-10% and 22% change, respectively). C: Same as in A for oocytes expressing MEC-4(d)+MEC-2 not treated (light red) and treated (dark red) with plasmin. N = 15 and 13 respectively. The difference between the means was 0.003 (0% change), the lower and upper 95% confidence levels were -0.391 and 0.398 respectively (-41% and 41% change; such large percentage changes are due to the relatively large variability of small currents). D: Same as panel A for oocytes expressing UNC-8(d)+MEC-2 not treated (light blue) and treated (dark blue) with plasmin. N = 6 and 5. The difference between the means was -0.87 (6% decrease), the lower and upper 95% confidence levels were -3.92 and 2.17 respectively (-27% and 15% change, respectively). E: Alignment of the finger domain of MEC-4 and UNC-8 containing the predicted plasmin sites (yellow background). F: Schematic representation of a DEG/ENaC channel subunit based on its crystal structure (Jasti, Furukawa et al. 2007). Alpha helixes are shown as cylinders and beta sheets are represented as flat arrows. The finger domain (purple) contains the plasmin recognition site (yellow box). The shaded area around transmembrane domains 1 and 2 (TM1 and TM2) represents plasma membrane.

Picture from Etchberger JF et al. (2007) Genes Dev "The molecular signature and cis-regulatory architecture of a C. elegans ...."

The srd-33 7TMR reporter gene fusion also shows a restricted expression pattern (ASEL/R, AWBL/R, ASHL/R, AVKL/R, PHAL/R, PHBL/R, and weakly in another unidentified tail neuron pair, possibly PHCL/R). Each data set of micrographs shows expression of the reporter construct under investigation in a high-magnification (400x) image, the overlap with a red fluorescent marker for ASEL/ASER (otIs151 transgene; 'ASE-RFP'), and a low-magnification (160x) overview of expression of the reporter construct under investigation throughout the whole worm. In the low-magnification images, the asterisk (*) indicates expression of the gut-specific injection marker elt-2::gfp.

Picture from Yugeta A et al. (2023) MicroPubl Biol "C. elegans ATG-5 mutants associated with ataxia."

(a) Amino acid sequence alignment among ATG5 orthologs from human (HsaATG5), Drosophila melanogaster (DmeAtg5) and Caenorhabditis elegans (CeleATG-5) using CLUSTALW. The red arrowhead indicates E122 in human ATG5.(b) Body length was measured in wild-type (N2) (N=30), atg-5(E121D) (N=28) and atg-5(E121A) (N=40) adult day 1 animals. Data from the all analyzed worms are plotted. Horizontal lines and error bars indicate means ± s.d.(c) Brood size of wild-type (N2) (N=9), atg-5(E121D) (N=9) and atg-5(E121A) (N=9) animals was determined by counting the number of fertilized eggs and offspring throughout their lifetime. Plots show the number of fertilized eggs per worm. Horizontal lines and error bars indicate means ± s.d.(d) Growth rate was analyzed in wild-type (N2), atg-5(E121D) and atg-5(E121A) animals. Approximately 100 eggs (WT (N=96), E121D (N=97), E121A (N=102)) were collected and the number of worms at each stage (egg, L1, L2−L3, L4, adult) was counted every 12 hours.(e) Autophagy flux was measured in wild-type (WT) (N=36), atg-5(E121D) (N=33), and atg-5(E121A) (N=37) animals expressing lgg-1p::gfp::lgg-1. Worms at the L4 stage were starved for 8 h to induce autophagy. The white arrowheads indicate LGG-1 punctae. Plots show the number of GFP::LGG-1 punctae in the pharynx. Horizontal lines and error bars indicate means ± s.d. Scale bar, 10 µm.(f) The frequency of body bends on adult days 3 and 7 in WT, atg-5(E121D) and atg-5(E121A) (N=30) animals. Plots show the frequencies of body bends. Horizontal lines and error bars indicate means ± s.d.n.s., not significant, *P < 0.05 by Dunnett's test.

Picture from Yin X et al. (2004) Mol Biol Cell "Inositol 1,4,5-trisphosphate signaling regulates rhythmic contractile ...."

Figure 6. Effect of dsRNA-induced disruption of plc-1 and plc-3 expression on sheath contractility. (A) Rates of basal sheath contraction in adult worms fed GFP, plc-1, or plc-3 dsRNA beginning at the L1 larval stage. Values are means ± SE (n = 7-29). *p < 0.01 compared with GFP dsRNA-fed worms. (B) Rates of basal and peak ovulatory sheath contractions in adult worms fed dsRNA for 16-24 h beginning in late-stage L4 larvae. Values are means ± SE (n = 8-13). *p < 0.05 and **p < 0.01 compared with GFP dsRNA-fed worms. (C) PLC-3::GFP expression pattern. Confocal differential interference contrast micrograph (left) and corresponding GFP fluorescence micrograph (right). GFP is expressed in proximal gonad sheath cells (arrowheads), spermatheca (Sp) and uterine sheath cells (arrow). DG, distal gonad; Int, intestine; Oo, oocyte.

Picture from Garzanelli, John et al. (2020) MicroPubl Biol

Percentage of embryos that failed to hatch after feeding RNAi knockdown in wildtype (A) or unc-33(e204) homozygous animals (B): Box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. Horizontal bars indicate the medians, asterisks indicate the means, and polygons represent density estimates of data and extend to extreme values. From left to right in the plot, n=24, 23, 24, 23, 23, 24, where each n is the percentage that failed to hatch from a single hermaphrodite. Standard deviations from left to right are 4.8, 14.8, 4.3, 19.2, 18.4, 30.9.

Picture from Lin L et al. (2013) J Cell Biol "The scaffold protein EPG-7 links cargo-receptor complexes with the autophagic ...."

At larval stages, W07G4.5::GFP forms aggregates in intestinal cells (Q and R).