Picture from Minniti AN et al. (2004) J Cell Sci "Caenorhabditis elegans syndecan (SDN-1) is required for normal egg laying and ...."

Figure 3. Heparan sulfate proteoglycans in C. elegans localize to the nerve ring, nerve cords and vulva. Whole worms were permeabilized, incubated with heparitinase and stained with anti-delta-heparan sulfate antibody. (a) Staining shows that heparan sulfate proteoglycans are located at the nerve ring (arrowheads), ventral nerve cord (white arrows) and dorsal nerve cord (gray arrows). (b) Heparan sulfate proteoglycans are also located in the vulva as seen from a ventral view (gray arrowheads). The white arrows indicate the ventral nerve cord and the gray arrows the dorsal nerve cord. (c) The staining of L2-L3 larva showed the location of heparan sulfate proteoglycans at the nerve ring (white arrowhead) and at the ventral and dorsal nerve cords (white and gray arrows, respectively). (d) The nervous system of a transgenic strain expressing the pan-neural GFP was visualized. (e) Heparan sulfate proteoglycans as seen in panel a were also immunodetected in this reporter strain. The white arrows indicate ventral nerve cord. (f) Merged images of panels d and e. The insert shows an enlarged area, with details of the neural bodies (green) and their close localization to the heparan sulfate proteoglycans (red). Controls without heparitinase treatment and without primary antibody are shown in panels g and h respectively. All images are 3D reconstructions of image stacks, except for d, e and f, which correspond to a single optic slice. Bar, 20 um.

Picture from Brockie PJ et al. (2001) J Neurosci "Differential expression of glutamate receptor subunits in the nervous system of ...."

C, D, Confocal images of a transgenic worm expressing glr-8::GFP in the pharyngeal nervous system (C, head region only) and in a single neuron in the tail (arrow; D). E, F, Neuronal cell bodies that express glr-8::GFP. Neurons filled in black coexpress glr-7::GFP and glr-8::GFP. Neurons filled in gray only express glr-8:: GFP in either the pharyngeal nervous system (E; URB is the only neuron of this subset that is not considered part of the pharyngeal nervous system) or the tail (F). Scale bars, 5 um.

Picture from Firestein BL et al. (2001) Mol Biol Cell "DLG-1 is a MAGUK similar to SAP97 and is required for adherens junction ...."

Figure 1. DLG-1 is a MAGUK protein most similar to SAP97. (A) Deduced amino acid sequence of DLG-1 with PDZ domains in blue, SH3 domain in red, and guanylate kinase domain in gray. (B) Alignment of the amino-terminal 99 amino acids of DLG-1 with SAP97. Identical residues are indicated in black, whereas conserved residues are indicated in gray. (C) Schematic diagrams of the DLG-1:: GFP chimeric proteins. Embryos expressing DLG-1::GFP (green) in intestine (D) and hypodermis (E) are shown with DAPI-stained nuclei (blue). Adult worms express DLG-1:: GFP in the Pnp epithelial cells (F) and lateral seam cells (G). Scale bar represents 10 um.

Picture from Firestein BL et al. (2001) Mol Biol Cell "DLG-1 is a MAGUK similar to SAP97 and is required for adherens junction ...."

Figure 1. DLG-1 is a MAGUK protein most similar to SAP97. (A) Deduced amino acid sequence of DLG-1 with PDZ domains in blue, SH3 domain in red, and guanylate kinase domain in gray. (B) Alignment of the amino-terminal 99 amino acids of DLG-1 with SAP97. Identical residues are indicated in black, whereas conserved residues are indicated in gray. (C) Schematic diagrams of the DLG-1::GFP chimeric proteins. Embryos expressing DLG-1::GFP (green) in intestine (D) and hypodermis (E) are shown with DAPI-stained nuclei (blue). Adult worms express DLG-1:: GFP in the Pnp epithelial cells (F) and lateral seam cells (G). Scale bar represents 10 um.

Picture from Luersen K et al. (2016) Genetics "Complex Locomotion Behavior Changes Are Induced in Caenorhabditis elegans by ...."

Figure S4. Expression pattern of TWK-7. (A-D) Confocal microscopy analysis of worms expressing the reporter proteins GFP and DsRed under the control of the cholinergic neuron specific unc-17(4.2kb) and the twk-7(3.0kb) promoter, respectively. The box in (A) marks the posterior part of the body shown in more detail in (B-D), where A-, B-, AS- and D-type motor neurons of the posterior ventral nerve cord are labeled according to their numbering on wormatlas.org. (E-G) Laser scanning confocal microscopy analysis of worms expressing GFP and a TWK-7::mCherry fusion protein under the control of the unc-17(4.2kb) and twk-7(3.0kb) promoter, respectively. Small white arrows exemplarily indicate motor neurons that contain cytosolic GFP and punctated expression of mCherry in the soma. Gray arrows point to D-type neurons that express solely mCherry.

Picture from Croft, Jaime C. et al. (2023) MicroPubl Biol

Figure 1. Lifespan extension in jhdm-1 mutants does not affect pumping rate, but long-lived populations stop pumping at a younger age:(A, B) Representative lifespan of jhdm-1(ok2364) mutants (blue) and generation-matched wild-type animals (gray) in early-gen (A, C, E) and late-gen populations (B, D, F). Lifespan was assessed for 3-4 independent transgenerational replicates, with one representative replicate shown here. (A) In early-gen F8 populations, jhdm-1 mutants are not long-lived. (B) In late-gen F13 populations, the median lifespan of jhdm-1 mutants was 5.26% longer than generation matched wild-type *p<0.05 with a long-rank test. (C, D) Pharyngeal pumping rate of jhdm-1 mutants (blue) in both early-gen (C) and late-gen (D) populations did not differ from generation matched wild-type animals (gray). Graphs combine data from three independent transgenerational replicates. Median is shown as a black line. (E, F) Percentage of the population pumping in jhdm-1 mutants (blue) and generation-matched wild-type (gray) across generational time. Graphs combine data from two independent transgenerational replicates. Total N values are shown in white. (E) Throughout adulthood, the percentage of early-gen jhdm-1 mutants that are pumping does not differ from wild-type. (F) In populations of late-gen jhdm-1 mutants that have acquired longevity, fewer animals are pumping in middle age (Day 8 of adulthood). *p<0.05 with a Fisher's exact test.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(D, D') Pgrd-10::GFP is expressed in seam cells; the mid-body region is shown.

Picture from Medley JC et al. (2023) MicroPubl Biol "kin-3 genetically suppresses sur-6 in centrosome assembly during ...."

Figure 1. kin-3(RNAi) suppresses embryonic lethality and abnormal centrosome behavior in sur-6(or550) mutant embryos:Genetic analysis of sur-6(or550) mutants at the semi-restrictive temperature (21°C, *** p<0.001) (B) Quantification of mitotic spindle formation (stacked bars) during the second cell cycle in temperature-sensitive sur-6(or550) mutants. At 22.5°C, sur-6(or550) embryos often display defective centrosome duplication, with monopolar (dark gray) or asymmetric bipolar (light gray) spindles during the second mitosis. kin-3(RNAi) increases the percentage of symmetric bipolar spindles (green) in sur-6(or550) embryos. n is the number of blastomeres scored. The bottom panel illustrates representative embryos expressing mCherry::tubulin, with symmetric bipolar (green arrows), asymmetric bipolar (light gray arrows), and monopolar spindles (dark gray arrowheads) at the second mitosis in sur-6(or550) mutants, indicative of defective centrosome assembly during the first cell cycle. (C) % Embryonic lethality of sur-6(or550) mutant animals at 22.5°C. Data are presented as mean±s.d. n is the number of progeny scored. (*** p<0.0001)

Picture from Lazakovitch E et al. (2005) BMC Dev Biol "nfi-I affects behavior and life-span in C. elegans but is not essential for DNA ...."

Figure 9. Expression pattern of the nfi-1::GFP reporter transgenes. The nfi-1 locus and structure of the nfi-1-GFP fusion con- structs are shown (A). Nfi-1 coding regions are shown in black, gray boxes indicate alternatively spliced exons, untranslated regions are in white. GFP is shown as a hatched box. Expression of nfi-1-GFP reporter constructs was observed in embryos (B), intestinal cells (C), body wall muscles (D, E), pharynx (F), egg-laying muscles (G), several head (H) and tail neurons (I). Expression was assessed using a FXA Nikon microscope (B-D, F-I) and a Bio-Rad confocal microscope (E).

Picture from Zhang D et al. (2012) J Cell Biol "RAB-6.2 and the retromer regulate glutamate receptor recycling through a ...."

Figure 10. RAB-6.2 and LIN-10 colocalize with RME-8 in neurons. (A-D) Fluorescence from neuron cell bodies (A and C) and ventral cord dendrites (B and D) is shown. (A-B'') Cerulean::RAB-6.2 (A and B) colocalizes with Venus::RME-8 (A' and B'; A'' and B'' show merged). (C-D'') LIN-10::CFP (C and D) colocalizes with Venus::RME-8 (C' and D'; C'' and D'' show merged). Arrowheads indicate colabeled puncta. Bars, 5 um.