Picture from Zheng Q et al. (2014) PLoS Genet "The vesicle protein SAM-4 regulates the processivity of synaptic vesicle ...."

Figure 2. sam-4 encodes a neuronally expressed protein SV associated protein. (A-C9) Distribution of SAM-4-TagRFP expressed in mechanosensory neurons in the synaptic varicosities (A) and the PLM soma and proximal section of the PLM neurite (C). GFP-RAB-3 localization is also shown in (B and B9) along with the merge (C and C9).

Picture from Tilmann C et al. (2005) Dev Cell "Cyclin D regulation of a sexually dimorphic asymmetric cell division."

Figure 2. q626 Feminizes the Gonad and Appearsto Function Like fkh-6 (A-C) L3 animals carrying anchor cell (AC) marker, cdh-3::GFP. (A) Wild-type hermaphrodite. (B) Wild-type male. (C) q626 male raised at the restrictive temperature expresses the AC marker. (D-F) L4 animals carrying marker for vas deferens (VD) and seminal vesicle (SV),K09C8.2::GFP. (D) Wild-type hermaphrodite. (E) Wild-type male. (F) q626 male raised at the restrictive temperature does not expressthe SV/VD marker. (G-I) L1 gonads viewed by Nomarski microscopy. Asterisks, germ cells. (G) Male SGP daughters differ in size. Small, open arrow, smaller distal daughter; large, open arrow, larger proximal daughter. (H) Hermaphrodite SGP daughters are of roughly equal size. White arrowhead, distal daughter; black arrowhead, proximal daughter. (I) q626 SGP daughters at the restrictive temperature are of roughly equal size. Same conventions as (H).

Picture from Smith JR et al. (2011) PLoS Genet "TRY-5 is a sperm-activating protease in Caenorhabditis elegans seminal ...."

try-5 is expressed in the male somatic gonad. Confocal and transmitted-light (TL) images of transgenic males bearing try-5 reporter insertions. In TL images, boundaries of regions of the somatic gonad are labeled as sv, seminal vesicle; vl, valve; and vas, vas deferens. Scale bars, 10 um. (A) jnSi49[Ptry-5::GFP::H2B]; try-5(tm3813) L4 male. In focal planes shown, GFP is visible in the four GFP-positive seminal vesicle cells, two of four GFP-positive valve cells, and six of twelve GFP-positive cuboidal cells. (B) jnSi62[Ptry-5::TRY-5::GFP]; try-5(tm3813) L4 male. TRY-5::GFP is concentrated at the apical side of seminal vesicle, valve and cuboidal cells.

Picture from Warren CE et al. (2002) J Biol Chem "The Caenorhabditis elegans gene, gly-2, can rescue the ...."

Figure 8. Pattern of gly-2promoter-directed GFP expression during nematode development. Except for panels F and G, images are presented as pairs showing the epifluorescence from the cytosolic GFP reporter in the upper section and differential interference contrast images from the same field and focal plane in the lower section. The emerald-green light is emitted by GFP, the yellow-green signal is gut autofluorescence. Scale bar in all cases is 20 μm.A, comma stage embryo. B, 1.5-fold stage embryo.C, 3-fold stage embryo. D, lateral view of an early larval stage 4 hermaphrodite peri-vulval region. DNC, dorsal nerve cord; VNC, ventral nerve cord; vul, vulval cells vul E and/or vul F. E, ventral view of late larval stage 4 hermaphrodite peri-vulval region. SV, spermathecal valve; v/u, vulval and/or uterine structures.F, as E, but from a nuclear-localized reporter.G, detail in lateral view of the spermathecal valve staining by the nuclear-localized reporter expressed in an adult hermaphrodite.H, lateral view of an adult hermaphrodite mid-body showing continued expression in the spermathecal valve (SV) and vulval and/or uterine structures (v/u). I and J, lateral views in two different focal planes from adult hermaphrodites showing vulval/uterine staining (v/u).K, lateral view of the pharynx from an adult hermaphrodite showing GFP fluorescence from neurons (N) in the nerve ring and ventral nerve chord (VNC) and the staining of the pharyngeal-intestinal valve (PIV).

Picture from Zheng Q et al. (2014) PLoS Genet "The vesicle protein SAM-4 regulates the processivity of synaptic vesicle ...."

Figure 2. sam-4 encodes a neuronally expressed protein SV associated protein. (A-C9) Distribution of SAM-4-TagRFP expressed in mechanosensory neurons in the synaptic varicosities (A) and the PLM soma and proximal section of the PLM neurite (C). GFP-RAB-3 localization is also shown in (B and B9) along with the merge (C and C9). (D-E) Western blots of fractionated whole worm extracts (D) and non-fractionated lysis (E) derived from animals expressing 3XFlag tagged SAM-4(+) and SAM-4(G2S) myristoylation mutant proteins probed for SAM-4, the synaptic vesicle protein SNB-1, and the cytosolic protein b-Tubulin. Note the shift in migration of the SAM-4(G2S) mutant. (F) Quantification of GFP-RAB-3 levels in PLM synaptic varicosities. **, P,0.001, relative to wild type; DD, P,0.001, relative to sam-4. Transgenes tested: jsIs1188 for sam-4(+), jsIs1265 for sam4(G2S). Allele tested: sam-4(js415). (G-H9) SAM-4(G2S)-TagRFP (jsEx1256) levels are reduced in PLM synaptic varicosities (G and H) but increased in PLM soma (G9 and H9), relative to SAM-4-TagRFP (jsIs1156). Solid arrow: PLM soma; open arrow: PLM neurite; solid arrowhead: PLM synaptic varicosity; open arrowhead: florescence marker puncta in PLM neurite; asterisk: vulva. Scale bars: 10 um.

Picture from Smith JR et al. (2011) PLoS Genet "TRY-5 is a sperm-activating protease in Caenorhabditis elegans seminal ...."

Figure 5. try-5 is expressed in the male somatic gonad. Confocal and transmitted-light (TL) images of transgenic males bearing try-5 reporter insertions. In TL images, boundaries of regions of the somatic gonad are labeled as sv, seminal vesicle; vl, valve; and vas, vas deferens. Scale bars, 10 mm. (A) jnSi49[Ptry-5::GFP::H2B]; try-5(tm3813) L4 male. In focal planes shown, GFP is visible in the four GFP-positive seminal vesicle cells, two of four GFP-positive valve cells, and six of twelve GFP-positive cuboidal cells. (B) jnSi62[Ptry-5::TRY-5::GFP]; try-5(tm3813) L4 male. TRY-5::GFP is concentrated at the apical side of seminal vesicle, valve and cuboidal cells. (C) jnSi62[Ptry-5::TRY-5::GFP]; try-5(tm3813) 48 hr adult male. Both large and small TRY-5::GFP globules are present in the cuboidal cells and valve region. In the seminal vesicle, TRY-5::GFP is present in the proximal region near the valve. (D) jnSi62[Ptry-5::TRY-5::GFP]; swm-1(me87) try-5(tm3813) 48 hr adult male. TRY-5::GFP has expanded into the seminal vesicle lumen and sperm are activated. (C9, D9) Images of seminal vesicle regions outlined in C and D with intensity levels optimized for the fainter GFP signal in these tissues. Arrows indicate GFP in the distal sheath-like cells of the seminal vesicle. (E) Paired DIC, epifluorescence and merged images of a jnSi62[Ptry- 5::TRY-5::GFP]; try-5(tm3813) 72 hr adult male in the region of the seminal vesicle. Localization of TRY-5::GFP is correlated with activated sperm (also see Table S4 and S5).