Picture from Arata, Yukinobu et al. (2020) MicroPubl Biol

Figure 1: Biofilm formation, the establishment of OP50-derived fliC and fimH knockout strains, and bacterial motility assay. Biofilm formation on glass (A) and polystyrene (B). Biofilm formed by indicated E. coli strains on glass and polystyrene quantified by CV staining. Optical absorbance at 600 nm was measured in three independent experiments and compared with paired student t-tests; *p < 0.05, ** p < 0.005. Biofilm formation ability of OP50 fliC::Km, OP50 fimH::Km, and BL21 should be studied further with rigorous experiments and statistical testing. (C and D) Isolation of OP50 fimH::Km and OP50 fliC::Km. (C) Schematic structure of fliC and fimH knockout alleles with position and direction of primers for PCR. Km cassette was integrated in gene locus to form fimH::Km and fliC::Km. (D) Confirmation of the presence of fimH::Km and fliC::Km in OP50-derived E. coli colonies via P1 transduction. Bacteria obtained from Km-resistant colonies were subjected to PCR with the indicated primers. DNA fragments of expected sizes from the genomic structure of the fliC::Km and fimH::Km knockout alleles were amplified with a primer set with K1 but not K2 primer. (E) Motility of E. coli strains. Motility of indicated E. coli strains was compared by colony size after cultivation on soft agar LB plates.