Picture from Qadota H et al. (2007) Mol Biol Cell "Two LIM domain proteins and UNC-96 link UNC-97/pinch to myosin thick filaments in ...."

Localization of LIM-8 is shown together with antibodies to markers for dense bodies (alpha-actinin) and M-lines (UNC-89). The left column shows LIM-8 localization. The center column shows dense body and M-line marker localization. The right column shows merged images of left and center columns. Rabbit antibodies were visualized by anti-rabbit IgG conjugated with Alexa 488, and mouse antibodies were visualized by anti-mouse IgG conjugated with Alexa 647.

Picture from Qadota H et al. (2007) Mol Biol Cell "Two LIM domain proteins and UNC-96 link UNC-97/pinch to myosin thick filaments in ...."

Localization of LIM-9 in adult body wall muscle cells. Localization of LIM-9 is shown together with antibodies to markers for dense bodies (alpha-actinin) and M-lines (UNC-89). The left column shows LIM-9 localization. The center column shows dense body and M-line marker localization. The right column shows merged images of left and center columns. Rabbit antibodies were visualized by anti-rabbit IgG conjugated with Alexa 488, and mouse antibodies were visualized by anti-mouse IgG conjugated with Alexa 647. White bar, 10 um.

Picture from Qadota H et al. (2007) Mol Biol Cell "Two LIM domain proteins and UNC-96 link UNC-97/pinch to myosin thick filaments in ...."

Figure 5. Localization of UNC-95, LIM-8, and LIM-9 in adult body wall muscle cells. Localization of UNC-95, LIM-8, and LIM-9 are shown together with antibodies to markers for dense bodies (alpha-actinin) and M-lines (UNC-89). The left column shows UNC-95, LIM-8, or LIM-9 localization. The center column shows dense body and M-line marker localization. The right column shows merged images of left and center columns. Rabbit antibodies were visualized by anti-rabbit IgG conjugated with Alexa 488, and mouse antibodies were visualized by anti-mouse IgG conjugated with Alexa 647. White bar, 10 um.

Picture from Sawa M et al. (2003) J Cell Sci "Essential role of the C. elegans Arp2/3 complex in cell migration during ventral ...."

Figure 9. Immunostaining by the anti-WSP-1 and anti-ARX-1 antibodies. (A) Anti-WSP-1 antibody conjugated with Alexa Fluor 546, (B) anti-ARX-1 antibody conjugated with Alexa Fluor 633, (C) expression of AJM-1::GFP and (D) the merged image are shown. WSP-1 is red, ARX-1 is blue and AJM-1 is green. The magnified image around the leading edge is shown in E, from left WSP-1, ARX-1, AJM-1 and the merged image. All images show the ventral view. The leading edge of the most anterior hypodermal cell is indicated with an arrowhead (A-E) and with a dotted line (E).

Picture from Walker CS et al. (2006) Proc Natl Acad Sci U S A "Conserved SOL-1 proteins regulate ionotropic glutamate receptor ...."

(G) Images of a transgenic worm that expressed GLR-1::CFP and STG-1::YFP in body wall muscle cells. (H and I) Images of transgenic worms injected with Alexa Fluor 594 conjugated anti-GFP that expressed either GFP::STG-1 (H) or STG-1::GFP (I) in body wall muscle cells.

Picture from Walker CS et al. (2006) Proc Natl Acad Sci U S A "Conserved SOL-1 proteins regulate ionotropic glutamate receptor ...."

Figure 4. GLR-1, SOL-1, and STG-1 are expressed on the surface of transgenic body wall muscle cells. (A) Schematic of body wall muscles, including muscle arms and the ventral cord. (B-F) Images of transgenic worm body wall muscle cells that expressed various combinations of HA::GLR-1::GFP, GFP::GLR-1::HA, GFP::SOL-1, SOL-1, and STG-1 as indicated using the myo-3 muscle-specific promoter. Transgenic worms were injected with Alexa Fluor 594 conjugated anti-HA or anti-GFP to detect surface expression of GLR-1 or SOL-1. (G) Images of a transgenic worm that expressed GLR-1::CFP and STG-1::YFP in body wall muscle cells. (H and I) Images of transgenic worms injected with Alexa Fluor 594 conjugated anti-GFP that expressed either GFP::STG-1 (H) or STG-1::GFP (I) in body wall muscle cells.

Picture from Loer, Curtis et al. MicroPubl Biol

Figure 1: FLAG antibody-stained P. pacificus worms using the HIAR-added protocol, as described in the text. Details on generating CRISPR/Cas9-mediated FLAG-epitope tagged alleles, and precise sites and developmental time courses of nuclear expression will be reported elsewhere. (A) Three Ppa-unc-3::2xFLAG larval worms (RS3506) stained with mouse anti-FLAG monoclonal antibody plus Alexa-594-conjugated anti-mouse secondary. (B) Staining of Ppa-unc-86::1xFLAG strain (RS3509) with anti-FLAG two tails (top), one head (bottom) of larve; antibodies as in A.

Picture from Gleason EJ et al. (2012) Genetics "Developmental genetics of secretory vesicle acidification during ...."

Figure 8: SPE-5 localization during spermatogenesis. Sperm cytology was visualized by DIC overlaid by DAPI staining of nuclei (blue on gray panels) or indirect immunofluorescence with either antibody 1CB4 that recognizes FB-MOs (Texas Red; red panels) or SPE-5 Mab enhanced by tyramine signal amplification (Alexa 488; green panels). For each field of cells, a z-series of fluorescent image slices in the DAPI, Texas Red and Alexa 488 channels was collected and each resulting wide field image was deconvolved. For each slice, DAPI, red and green channels were overlaid to create a triple labeled slice. All triple labeled slices for each field of cells were also stacked to create a through-field projection image stack (far right column of images). A, B, C and E are dpy-5 him-5 control (23 plane slices, slice #16 from the bottom shown), D is him-8 control (21 plane slices, slice #16 from the bottom shown) and F is spe-5(ok1054) him-8 (18 plane slices, slice #10 shown). Differentiation stages: (A) primary spermatocytes, (B and C) secondary spermatocytes, (D) budding spermatids, (E) budded spermatids and (F) spe-5 terminally arrested spermatocytes. Arrows indicate SPE-5 accumulation in dots. For D and E, arrows point to SPE-5 accumulation that is close to MOs. RB = residual body. The scale bar is 5 um for each row of images.

Picture from Kinnaird JH et al. (2004) Exp Cell Res "HRP-2, a heterogeneous nuclear ribonucleoprotein, is essential for ...."

Fig. 2. HRP-2 is expressed in nuclei of all life cycle stages of C. elegans. (A-E) GFP fluorescence in different life cycle stages of transgenic line HRP-2DGFP; (A-C) Upper panel, fluorescence; lower panel, DIC. (A) Uterus from an adult hermaphrodite showing fluorescence in early embryos; (B) embryos at three-fold stage showing large gut cell nuclei; (C) L2 with fluorescence in most tissues particularly bright around the pharynx; (D) diffuse nuclear staining in gut cell from isolated adult intestine; (E) punctate staining in neuronal cell nuclei. (F-H) Immunofluorescence with R64 Ab and prebleed. Panel F shows colocalisation of HRP-2 and DAPI staining in tail region of freeze-cracked and fixed L2 stained with DAPI (top), 1:1000 R64 Ab (middle) and merge (bottom). R64 Ab was detected with antirabbit Alexa 488 at 1:300 dilution. Panel G shows HRP-2 R64 reactivity with gut and gonad dissected from adult hermaphrodite. R64 Ab (upper) and DAPI (lower); (H) similar specimen stained with 1:1000 prebleed (upper) and DAPI (lower).