Picture from Ailion M et al. (2014) Neuron "Two Rab2 interactors regulate dense-core vesicle maturation."

(C) An L4 stage late-larval animal is shown. Pcccp-1::GFP is expressed throughout the nervous system, including head and tail neurons and motor neurons in the ventral cord. Expression is also seen in the intestine, but is not seen in pharynx, skin or muscle cells. This animal has intestinal expression in only the posterior intestine due to mosaicism of the extrachromosomal array. Scale bar: 50 um.

Picture from Ailion M et al. (2014) Neuron "Two Rab2 interactors regulate dense-core vesicle maturation."

Figure S4. rund-1 and cccp-1 are expressed widely in neurons and other tissues.Expression of rund-1 and cccp-1 promoter::GFP fusions are shown in transgenic animals carryingextrachromosomal arrays.(A) An L4 stage late-larval animal is shown. Prund-1::GFP is expressed throughout the nervoussystem, including head and tail neurons and motor neurons in the ventral cord. Expression is also observed in the pharynx and intestine, but not in skin or muscle cells. This animal has intestinalexpression in only the posterior intestine due to mosaicism of the extrachromosomal array. Scale bar: 50 µm.(B) Expression of Prund-1::GFP in the spermatheca, uterus and ventral nerve cord (arrowheads) of a gravid adult. GFP and DIC images are superimposed. Scale bar: 10 µm.(C) An L4 stage late-larval animal is shown. Pcccp-1::GFP is expressed throughout the nervous system, including head and tail neurons and motor neurons in the ventral cord. Expression is alsoseen in the intestine, but is not seen in pharynx, skin or muscle cells. This animal has intestinal expression in only the posterior intestine due to mosaicism of the extrachromosomal array. Scale bar: 50 µm.

Picture from Ailion M et al. (2014) Neuron "Two Rab2 interactors regulate dense-core vesicle maturation."

(A) An L4 stage late-larval animal is shown. Prund-1::GFP is expressed throughout the nervous system, including head and tail neurons and motor neurons in the ventral cord. Expression is also observed in the pharynx and intestine, but not in skin or muscle cells. This animal has intestinal expression in only the posterior intestine due to mosaicism of the extrachromosomal array. Scale bar: 50 um.(B) Expression of Prund-1::GFP in the spermatheca, uterus and ventral nerve cord (arrowheads) of a gravid adult. GFP and DIC images are superimposed. Scale bar: 10 um.

Picture from Ailion M et al. (2014) Neuron "Two Rab2 interactors regulate dense-core vesicle maturation."

(C) CCCP-1 colocalizes with RUND-1 and RAB-2. CCCP-1 colocalizes with RUND-1 and RAB-2(GTP). CCCP-1 is still punctate when coexpressed with RAB-2(GDP), which is diffusely expressed.

Picture from Ailion M et al. (2014) Neuron "Two Rab2 interactors regulate dense-core vesicle maturation."

(B) RUND-1 localizes to the Golgi. Left: a backscatter scanning electron micrograph of the cell body of a neuron in the nerve ring. Right: the same image overlaidwith the corresponding fluorescence PALM image of RUND-1::tdEos. Scale bar, 1 mm.

Picture from Ailion M et al. (2014) Neuron "Two Rab2 interactors regulate dense-core vesicle maturation."

Figure 5. RUND-1 Colocalizes with RAB-2 at the trans-Golgi Network(A) RUND-1 colocalizes with RAB-2 and trans-Golgi markers. Each panel shows a single slice of a confocal image of motor neuron cell bodies in the ventral cord of young adult animals. The top boxes show the localization of a single-copy rescuing RUND-1::tagRFP-T fusion protein (oxIs590). RUND-1 localizes almost exclusively to two or three perinuclear puncta per cell. The middle boxes show the localization of single-copy GFP-tagged compartment markers. The bottom boxes show the merged images. Scale bar, 5 μm, applies to all panels. RUND-1 shows tightest colocalization with RAB-6.2, a trans-Golgi Rab protein, and with SYX-6, the ortholog of the trans-Golgi SNARE syntaxin 6. RUND-1 puncta also colocalize well with RAB-2 puncta. RUND-1 partially overlaps with the medial Golgi marker mannosidase II (AMAN-2) and the cis-Golgi marker µCOP. RUND-1 is not colocalized with the rough ER marker TRAM-1 or with the endosomal markers RAB-5, RAB-11.1, RAB-7, and SYN-13.(B) RUND-1 localizes to the Golgi. Left: a backscatter scanning electron micrograph of the cell body of a neuron in the nerve ring. Right: the same image overlaidwith the corresponding fluorescence PALM image of RUND-1::tdEos. Scale bar, 1 μm.(C) CCCP-1 colocalizes with RUND-1 and RAB-2. CCCP-1 colocalizes with RUND-1 and RAB-2(GTP). CCCP-1 is still punctate when coexpressed with RAB-2(GDP), which is diffusely expressed.(D) The RUN domain of RUND-1 mediates its localization. Full-length RUND-1, the coiled-coil domain, and the RUN domain were tagged at their C termini with tagRFP-T and integrated in the genome. The truncated proteins were expressed at lower levels. See also Figure S8.

Picture from Goh KY et al. (2018) Biochim Biophys Acta "A large transcribed enhancer region regulates C. elegans bed-3 and the ...."

(A) bed-3 is expressed in the M lineage including M descendants and SM descendants.

Picture from Yang Q et al. (2017) Dev Cell "The Invading Anchor Cell Induces Lateral Membrane Constriction during Vulval ...."

(M-P) Localization of the endogenous GFP::TOCA-1 reporter zh110 in wild-type (M) and unc-6(ev400) mutants (O and P) before (M and O) and after (N and P)lumen formation. (M') and (O') show yz projections of the lateral VulF membranes. The inset in (O) shows the displaced AC in the unc-6(ev400) mutant in a differentfocal plane.

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).