Figure 3. NCK-1 co-localizes with VAB-1. Anterior is to the left in all panels. (A-B) NCK-1 is in Green and VAB-1 is Red. (A) NCK-1 and VAB-1 colocalized in some cells. Arrow head points to cells where both NCK-1 and VAB-1 are co-localized, and the arrow points to cells that only express NCK- 1. The translational
nck-1::gfp transgene encodes the NCK-1A isoform and was detected using anti-GFP antibodies. Endogenous VAB-1 was detected using anti-VAB-1 antibodies (see experimental procedures). (B) NCK-1 and VAB-1 co-localized in the cell body and axon of the PLM neurons. nck- 1A::gfp and myr-
vab-1 were expressed in the PLM under the
mec-4 promoter, and were detected using anti-GFP and anti-VAB-1 antibodies respectively.