Males receive copulatory plugs from other males, deposited on their excretory pores. This phenotype is polymorphic among C. elegans strains and also in C. briggsae.
Failure in directed movement in response to sodium acetate. In C. elegans, sodium acetate is an attractant. Sodium acetate has also subsequently been used to assay for Na+ attraction in C. elegans.
Failure in directed movement in response to ammonium chloride compared to control. In C. elegans, NH4Cl is an attractant. NH4Cl has also subsequently been used to assay for Cl- attraction in C. elegans.
Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die.
Animals exhibit variations in the entry into the dauer stage when exposed to temperatures that induce dauer formation in control animals. In C. elegans, many Daf-c animals enter dauer at 25C, whereas Hid animals enter dauer at 27C.