Ali Khan L et al. (1994) Worm Breeder's Gazette "cej-1 Encodes a Novel Protein With Poly-Threonine Motif"

Siddiqui SS, Fukushige T, Ali Khan L, Tsukita Sh, Tabish M, Itoh M

[Worm Breeder's Gazette, 1994]

cej-1 Encodes a Novel Protein with Poly-Threonine Motif M. L. A. Khanl, M. Tabish, T. Fukushigel1 S. Tsukita2, M. Itoh , Sh. Tsukita , and S. S. Siddiqui. (1): Lab. of Molecular Biology, Dept of Ecological Engg. Toyohashi Univ. Technology, Toyohashi 441, and (2). National Institute for Physiological Sciences, Okazaki 444, Japan.

Bakaev VV (1996) Worm Breeder's Gazette "AN ATTEMPT TO SLOW AGING IN C. elegans. 3.THE EFFECT OF EXTRACT FROM YOUNG WORMS DURING POSTREPRODUCTIVE PERIOD"

Bakaev VV

[Worm Breeder's Gazette, 1996]

C. elegans (1 - 2 days old) were collected from separate stock, frozen and after 12 hours or more time, thawed, filtrated and mixed with S medium containing E. coli (1:1), this mixture was used as a medium for worms in experimental group. The medium for control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and with or without young worms extract) during 12 hours, then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). The medium with extract from young worms was used in experimental group beginning from 12th day, that is practically in postreproductive period of nematode life span. A number of progeny was calculated every day. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.

Kitamura S et al. (1994) Worm Breeder's Gazette "The Troponin C Gene, tnc-1, of Caenorhabditis elegans: Genome Structure and Mutation Sites of pat-10"

Williams BD, Kitamura S, Kagawa H, Sakube Y, Matsumoto S

[Worm Breeder's Gazette, 1994]

The troponin C gene, tnc-1 of Caenorhabditis elegans: Genome structure and mutation sites of pat-10 S. KITAMURA, B. WILLIAMS*, Y. SAKUBE, S. MATSUMOTO and H. KAGAWA, Dept. of Biol., Fac. of Sci., Okayama University, Japan, 700. *;Dept. of Gen., Washington Univ. School of Med., St. Louis

Salser SJ et al. (1994) Worm Breeder's Gazette "mab-5 Expression Repeatedly Switches ON and OFF in the V5 Lineage"

Kenyon CJ, Salser SJ

[Worm Breeder's Gazette, 1994]

mab-5 Expression Repeatedly Switches ON and OFF in the V5 Lineage S. Salser and C. Kenvon, UCSF, San Francisco, CA. 94143

Bakaev VV (1995) Worm Breeder's Gazette "AN ATTEMPT TO SLOW AGING IN C. elegans. 2. THE EFFECT OF EXTRACT FROM YOUNG WORMS DURING REPRODUCTIVE AND POSTREPRODUCTIVE PERIODS"

Bakaev VV

[Worm Breeder's Gazette, 1995]

C. elegans (1 - 2 days old) were collected from separate stock, frozen and after 12 hours or more time, thawed, filtrated and mixed with S medium containing E. coli (1:1), this mixture was used as a medium for worms in experimental group. The medium for control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and with or without young worms extract) during 12 hours then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). The medium with extract from young worms was used in experimental group beginning from third day, that is in reproductive and postreproductive periods of nematode lie span. Number of progeny was calculated every day. This investigation was carried out in temperature +21 C and in the dark- ness. The obtained results are presented in the following table. ...................................................................... Control group Experimental group n=11 P n=10 Mean+/-S.D. Mean+/-S.D. ......................................................................... Longevity: mean 19,2 +/- 1,9 >0,05 24,2 +/- 2,4 maximal 30 35 minimal 11 11 Period: prereproductive 3,4 +/- 0,3 >0,05 2,9 +/- 0,1 reproductive 10,2 +/- 0,9 <0,05 14,0 +/- 1,3 postreproductive 4,8 +/- 1,2 >0,05 6,9 +/- 1,7 Fecundity: mean 146,8 +/- 10,3 <0,02 199,4 +/-16,2 maximal 182 330 minimal 75 113 ...................................................................... Conclusion: If the extract from young worms was applied to C. elegans during reproductive and postreproductive period of their life span, then it was able to increase their mean fecundity (by 35,8 per cent) as well as length of reproductive period (by 37,3 per cent). The mean longevity in experimental group was slightly increased too (but not significantly). Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E.coli OP50.

Rosenberg R (1978) Worm Breeder's Gazette

Rosenberg R

[Worm Breeder's Gazette, 1978]

All eyes are on the newest fashion trend, the Dumpy Look . Pace setting designer I.M. Worm s androgynous wardrobe is all the rage in Paris. Bianca Jagger quips, Tres, tres - Women s Wear Daily writes, Elegans personified - Patti Smith thinks, The punks won t buy it and Craig Russell says, It fits right in with my act . A product of Mutant Isolation, Inc.

Musset-Bilal F et al. (1994) Worm Breeder's Gazette "Characterization of the C. elegans Basement Membrane-Associated Protein SPARC"

Musset-Bilal F, Schwarzbauer JE, Ryan CS

[Worm Breeder's Gazette, 1994]

Characterization of the C. elegans Basement Membrane- Associated Frederique Musset-Bilal, Carol S. Ryan, and Jean E. Schwarzbauer Department of Molecular Biology, Princeton University, Princeton NJ 08544

Simske JS et al. (1994) Worm Breeder's Gazette "Specification of Vulval Cell Fates By Sequential Signalling Pathways."

Simske JS, Kim SK

[Worm Breeder's Gazette, 1994]

Specification of vulval cell fates by sequential signaling pathways Jeffrey S. Simske and Stuart K. Kim, Dept. of Developmental Biology, Stanford University Medical Center, Stanford CA 94305

Cruzen M et al. (1987) Worm Breeder's Gazette "preparation and maintenance of age-synchronous mass cultutes."

Johnson TE, Cruzen M

[Worm Breeder's Gazette, 1987]

For biochemical analyses of aging it is sometimes necessary to have gram quantities of material. We have devised a protocol for maintaining large numbers of age-synchronous worms in mass culture that provides gram quantities of aged worms. We faced two problems in generating large cultures: 1) worms must be synchronized and maintained in synchrony uncontaminated by progeny; 2) dead worm carcasses and other unwanted contaminants must be disposed of. Cultures are initially grown at 20 C on NGM then transferred to large NGM plates supplemented with egg yolk. Worms on these plates are allowed to grow to confluency and harvested to maximize the number of fecund adults. Age-synchronous cultures are initiated by treating fecund adults with a hypochlorite solution, washing the resultant eggs in S-basal three times, and allowing them to hatch overnight in S- basal. L1's are then counted by making serial dilutions and transferred at a concentration of 3x10+E4 L1's per plate to 2% peptone plates spread with RW2, a prototrophic strain of E. coli. The worms are kept on 2% peptone plates until they are young adults (40 hrs at 25 C after plating as L1's). Worms are suspended in S-basal at 10 worms/ml and transferred to large petri plates in 2x10+E9 E.coli/ml. Synchrony of the mass cultures is maintained by sterilization using fer-15 (b26,ts) grown at 25 C. We are also experimenting with the use of DNA synthesis inhibitors (e.g. FUdR) and brute force filtration to remove contaminant progeny using a 30 nylon mesh filter. Worm carcasses are removed by suspending the worms in S-basal, layering over an equal volume of Sepracell-MN (1.045 g/cm ) and centrifuging for 5 minutes at 500g. Live worms are found in the pellet while the carcasses remain at the interface. When maintaining the worms in liquid medium, ciliate contamination is sometimes a problem. This can be remedied by treating the entire population with . 025% detergent (Dove) and subsequently washing two times in S-basal with no apparent effect on the worms. Mean life spans of populations ( 25 worms) of DH26 with or without detergent treatment were 9.2 and 10. 1 days, respectively at 25 C. We are currently using these techniques to examine lysosomal enzyme specific activity and oxygen consumption in aging worms.

Bakaev VV (1995) Worm Breeder's Gazette "AN ATTEMPT T0 SLOW AGING IN C. elegans. I. THE EFFECT OF EXTRACT FROM YOUNG WORMS DURING WHOLE LIFE SPAN"

Bakaev VV

[Worm Breeder's Gazette, 1995]

C. elegans (1-2 days old) were collected from separate stock, frozen and after 12 hours or more time thawed, filtrated and mixed with S medium containing E. coli (1:1), this mixture was used as a medium for worms in experimental group. The medium for control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3-5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and with or without young worms extract) during 12 hours, then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). Number of progeny was calculated every day too. This experiment was carried out in temperature 21 C and in the darkness. The obtained results are presented in the following table. ............................................................... Control group Experimental group n = 12 P n = 11 Mean +/- S.D. Mean +/- S.D. Longevity: mean 24,5 +/- 1,9 <0,001 13,6 +/- 1,5 maximal 36 21 minimal 13 8 Period: prereproductive 3,9 +/- 0,08 <0,05 4,4 +/- 0,2 reproductive 9,9 +/- 0,09 <0,01 6,4 +/- 1,2 postreproductive 9,9 +/- 1,8 <0,001 2,8 +/- 0,7 Fecundity: mean 123,9 +/- 12,7 <O,001 33,6 +/- 7,6 maximal 219 69 minimal 13 o .................................................................. Conclusion: if the extract from young worms was applied to C. elegans during whole life span then it was able to decrease longevity of nematodes, by other words such an extract appeared as toxic, in indicated above conditions. This extract decreased the fecundity too. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP 50.