Ward S (1980) Worm Breeder's Gazette

Ward S

[Worm Breeder's Gazette, 1980]

If you are peacefully picking worms one day and an eight-legged hairy monster walks into the field of your dissecting microscope, after you come down from the ceiling, you will know that you too have a mite infestation. You will also know the plot for a grade B horror flick. A piece of the moth crystals used in closets (paradichlorobenzene) placed in your incubator for a few weeks will kill them off with no detectable harm to worm stocks.

Jin YS et al. (1994) Worm Breeder's Gazette "unc-25 Encodes a C. elegans Glutamic Acid Decarboxylase (GAD)"

Horvitz HR, Jin YS

[Worm Breeder's Gazette, 1994]

unc-25 encodes a C elegans glutamic acid decarboxylase (GAD) Yishi Jin and Bob Horvitz, HHMI, Dept. Biology, Mrr, Cambridge, MA 02139, USA

Goff SP (1999) Cancer Research "Genetic control of programmed cell death in the nematode Caenorhabditis elegans - Introduction."

Goff SP

[Cancer Research, 1999]

It is an honor and a great pleasure to introduce Dr. Robert Horvitz to you as the 1998 recipient of the Alfred Sloan Prize of the General Motors Cancer Research Foundation. Let me begin by telling you a little bit about Bob's

Tatar M et al. (2002) Science "Aging. Dietary advice on Q."

Rand DM, Tatar M

[Science, 2002]

How much you eat, not what you eat, seems to make a difference in the aging process. It is well established that reduced calorie consumption robustly extends adult life expectancy in a variety of animal models. Now, on page 120 of this issue, Larsen and Clarke show that diet quality also affects aging. In the worm Caenorhabditis elegans, reduced consumption of coenzyme Q (Q) dramatically extends longevity.

Karen Yook (2005) WormBook "Complementation"

Karen Yook

[WormBook, 2005]

Mutations in many genes can result in a similar phenotype. Finding a number of mutants with the same phenotype tells you little about how many genes you are dealing with, and how mutable those genes are until you can assign those mutations to genetic loci. The genetic assay for gene assignment is called the complementation test. The simplicity and robustness of this test makes it a fundamental genetic tool for gene assignment. However, there are occasional unexpected outcomes from this test that bear explanation. This chapter reviews the complementation test and its various outcomes, highlighting relatively rare but nonetheless interesting exceptions such as intragenic complementation and non-allelic non-complementation.

Coulson AR et al. (1989) Worm Breeder's Gazette "genome map."

Ameer H, Albertson DG, Sulston JE, Coulson AR, Waterston RH, Fishpool RM

[Worm Breeder's Gazette, 1989]

The map is now widely distributed electronically (see WBG 10(3), 67), but we are once again providing a summary for the gazette in the form of an output from the routine CHPLT. Do note that this is a provisional best guess, and that some linkages may later go away: please enquire if you need to know about the status of particular areas. When you receive cosmid clones, as stabs, please IMMEDIATELY streak them out on selective medium, pick small colonies, and grow 4ml minipreps (protocol from Alan Coulson if needed). For some cosmids, larger preps are liable to yield deleted DNA. Check that cosmid DNA appears full size (runs slower than lambda on agarose gels), then freeze a sample of good cells in 20% glycerol at -70 C. MRC computer account 'ARC' does not exist; Alan and John share account JES. A database node is now open at Seattle: modem number 206-467-2957; operator Phil Meneely. The summary of clone types given on the next page may be helpful when you are deciding which clones to request for your research. To reveal the most suitable clones for microinjection, the buried clones need to be displayed by the routine CONTASS; we will help you to do this if you ask. [See Figures 1- 3]

Todd Harris et al. (2007) International Worm Meeting "Your own private WormBase."

Todd Harris, Lincoln Stein

[International Worm Meeting, 2007]

Imagine being able to run WormBase on your own computer, unencumbered from server load at www.wormbase.org, network traffic, or even the need for a network connection. Run BLAST searches on your laptop enroute to your next presentation or explore expression patterns of your favorite gene family at the corner coffee shop. Maybe you have a private dataset that you would like to incorporate with WormBase to share within your lab or organization. Or perhaps you would like to set up a mirror site of WormBase as a community resource. All of these scenarios are easily possible with WormBase packages called "virtual machines". Virtual machines contain the entirety of WormBase and an operating system to hold everything together. In essence, this WormBase-customized operating system runs safely inside your Mac (Intel), Windows, or Linux computer using free software from VMWare. Virtual machines are now created for every WormBase release. Learn more about how to use these on the WormBase Wiki: http://www.wormbase.org/wiki/index.php/Virtual_Machines Bring your laptop by this poster and we will install the most recent version of WormBase for you!

Fire A et al. (1997) International C. elegans Meeting "SOME PROMOTER ACTIVITY PATTERNS"

Hsieh J, Macarah C, Fire A, Kelly B, Harfe BD, Montgomery MK, Xu SQ

[International C. elegans Meeting, 1997]

We will describe a variety of interesting germline and somatic promoter activity patterns and make some suggestions as to how you might be able to engineer your gene to be expressed in any of all of these patterns.

Howell K et al. (2016) Neuron "Small Immunoglobulin Domain Proteins at Synapses and the Maintenance of Neuronal Features."

Hobert O, Howell K

[Neuron, 2016]

The integrity of neural circuits must be maintained throughout the lifetime of an organism. In this issue of Neuron, Cherra and Jin (2016) characterize a small, two-Ig domain protein, ZIG-10, and its role in maintaining synaptic density in a specific set of C.elegans neurons.

Shakes DC (1993) Worm Breeder's Gazette "Call for Antibody Information"

Shakes DC

[Worm Breeder's Gazette, 1993]

As some of you are already aware, Academic Press has asked Henry Epstein and myself to edit a volume of Methods in Cell Biology devoted to C. elegans. One of the useful appendices which we would like to include is a list of available antibodies and protocols for using them. If you have generated an antibody in your laboratory which you would like to see listed in a methods volume on C. elegans, please contact me as soon as possible. Incidentally, if you would like to make your antibodies widely available but are nervous about being swamped with requests, you might considered contributing your monoclonal antibodies to the Developmental Studies Hybridoma Bank sponsored by the National Institute of Child Health and Human Development. They are providing the wonderful service of growing up monoclonals and making the cells or supernatants available to researchers at reduced fees. I know from personal experience that their anti-tubulin antibody works well for C. elegans, and it is considerably cheaper than commercial products. Contact Dr. Thomas August, Johns Hopkins University at (410) 955-3985 for more information about receiving a catalog or making contributions. I have the impression that they are not anxious to handle hundreds of worm specific antibodies, but that they are interested in handling a limited set of the most widely used C. elegans specific antibodies as well as antibodies which are known to cross-react with other species.