Akerib, Chantal C et al. MicroPubl Biol

Yamaya, Kei, Villeneuve, Anne M, Strand, Liesl G, Nsamba, Emmanuel T, Yokoo, Rayka, Akerib, Chantal C

[MicroPubl Biol, 2022]

RAD-54.L is required for the repair of meiotic double-strand DNA breaks (DSBs), playing an essential role in promoting removal of recombinase RAD-51 and normal completion of meiotic recombination. Failure to complete meiotic DSB repair leads to 100% lethality of embryos produced by rad-54.L null mutant mothers. Here we report a new partial loss of function allele, rad-54.L(me139) , that may prove useful for investigating meiotic mechanisms by providing a sensitized genetic background that reduces but does not eliminate the essential functions of RAD-54.L.