Wolfgang Link et al. (2001) International C. elegans Meeting "A transgenic C. elegans model for Parkinsons Disease"

Marius C Hoener, Wolfgang Link, Ralf Baumeister, Karlheinz Tovar

[International C. elegans Meeting, 2001]

Parkinson's Disease (PD) is the second most common neurodegenerative disorder with resting tremor, muscle rigidity, bradykinesia and postural instability as primary diagnostic signs. The movement dysfunction results from specific degeneration of dopaminergic neurons in the substantia nigra. The neuropathological hallmark of PD is the Lewy body, a cytoplasmic inclusion largely composed of fibrils formed by the polymerized, presynaptic protein alpha-synuclein. The point mutations A53T and A30P in the alpha-synuclein gene are linked to an autosomal dominant form of familial PD suggesting that a toxic gain of function may participate in the pathophysiology of PD. As C. elegans has shown to be a powerful model organism for the study of human neurological diseases like Alzheimer's Disease and Huntington's Disease, we established a nematode model for PD based on the expression of human alpha-synuclein. We generated worms carrying extrachromosomal and integrated arrays of human wildtype and mutant alpha-synuclein controlled by the C. elegans promoters unc-119 and sel-12 . Transgenic worms containing constructs expressing alpha-synuclein in neurons produce axonal deposits with strong anti alpha-synuclein immunoreactivity implying transport to the presynapse and aggregation in the axon. Furthermore, we detected a high molecular alpha-synuclein aggregate that migrated in the stacking gel in immunoblotted worm protein extracts. Transgenic C. elegans expressing human alpha-synuclein display noticeable phenotypes including locomotor dysfunction, egg laying defect and morphological abnormalities suggesting alpha-synuclein toxicity. As it has been reported for the Drosophila model of PD we did not detect major phenotypic differences between worms containing wild type or mutant alpha-synuclein. Experiments to determine the fate of the dopaminergic neurons and the structure of alpha-synuclein deposits in the transgenic animals are in progress. In summary, we have created a transgenic model of PD that replicates key features of PD pathology.

Elouej S et al. (2020) Nat Commun "Author Correction: Loss of MTX2 causes mandibuloacral dysplasia and links mitochondrial dysfunction to altered nuclear morphology."

Lenaers G, Nampoothiri S, Pereira C, Menabawy NA, Rubinsztajn R, Selim L, Loh A, Deleuze JF, De Sandre-Giovannoli A, Bartoli C, Harhouri K, Elouej S, Paneque AL, Airault C, Escande-Beillard N, Mao ML, Baujat G, Kubisch C, Reversade B, Gruenbaum Y, Martino L, Lessel D, Bauer P, Muchir A, Kayserili H, Charar C, Manivet P, Rotig A, Levy N, Lee SH

[Nat Commun, 2020]

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Verma S et al. (2020) Commun Biol "Author Correction: Diethylcarbamazine activates TRP channels including TRP-2 in filaria, Brugia malayi."

Kashyap SS, Robertson AP, Martin RJ, Verma S

[Commun Biol, 2020]

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Burns AR et al. (2020) Nat Commun "Author Correction: Caenorhabditis elegans is a useful model for anthelmintic discovery."

Giaever G, Roy PJ, McQuibban GA, MacRae CA, Musso G, Fraser AG, Yeo M, Rajendran L, Caffrey CR, Burns AR, Zhang Y, Stasiuk S, Redman E, Hunter R, Luciani GM, Schertzberg M, Gilleard J, Nislow C, Cutler SR, Bagg R, Glavin J, Tyers M

[Nat Commun, 2020]

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Kim J et al. (2019) Nat Methods "Publisher Correction: Oblique-plane single-molecule localization microscopy for tissues and small intact animals."

Marnani N, Flannery JG, Zhang X, Kim J, Zin EA, Newman ZL, Moon S, Wojcik M, Xu K, Wang Y

[Nat Methods, 2019]

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Koyuncu S et al. (2020) Nat Commun "Author Correction: The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington's disease patients."

Saez I, Fatima A, Vilchez D, Gutierrez-Garcia R, Hoppe T, Pokrzywa W, Lee HJ, Koyuncu S

[Nat Commun, 2020]

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Mugahid DA et al. (2020) Sci Rep "Author Correction: Proteomic and Transcriptomic Changes in Hibernating Grizzly Bears Reveal Metabolic and Signaling Pathways that Protect against Muscle Atrophy."

Tursun B, Kempa S, Gesell-Salazar M, Nelson L, Sengul TG, Voelker U, Bergmann N, Robbins CT, Wang Y, You X, Balogh A, Ofenbauer A, Mugahid DA, Chen W, Steil L, Gotthardt M, Radke MH

[Sci Rep, 2020]

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Putnam A et al. (2020) Nat Struct Mol Biol "Author Correction: A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos."

Cassani M, Seydoux G, Smith J, Putnam A

[Nat Struct Mol Biol, 2020]

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Sfakianos AP et al. (2020) Cell Death Differ "Correction to: The mTOR-S6 kinase pathway promotes stress granule assembly."

Whitmarsh AJ, Mellor LE, Sfakianos AP, Ashe MP, Kritsiligkou P, Poulin GB, Abou-Hamdan H, Pang YF, Needs H, Desaubry L

[Cell Death Differ, 2020]

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Akduman N et al. (2020) ISME J "Correction: Bacterial vitamin B12 production enhances nematode predatory behavior."

Roedelsperger C, Lightfoot JW, Roeseler W, Sommer RJ, Witte H, Lo WS, Akduman N

[ISME J, 2020]

An amendment to this paper has been published and can be accessed via a link at the top of the paper.