Wang XY et al. (2007) Ying Yong Sheng Tai Xue Bao "[Toxicity evaluation of purified terephthalic acid wastewater by using model Caenorhabditis elegans]"

Zhang XX, Yu HX, Cheng SP, Wang DY, Wang XY, Wang XR

[Ying Yong Sheng Tai Xue Bao, 2007]

By using model Caenorhabditis elegans, the toxicity of purified terephthalic acid (PTA) wastewater was evaluated through a battery of biotest, including life span, days of 50% lethal, generation time, brood size, head thrashes, and body bends. The results revealed that compared with control, the life span and generation time of C. elegans exposed to 660 mg PTA x L(-1) were delayed to some degree, and its head thrashes and body bends were inhibited. The procreation function of C. elegans exposed to PTA wastewater was greatly affected, with the brood size being as low as 25% of the normal one. The most sensitive indicator, brood size of C. elegans, might be a potential indicator in evaluating PTA wastewater toxicity.

Wang XY et al. (2007) Huan Jing Ke Xue "[Evaluation of toxification in acid and alkalescent industry liquid wastes from paper mill using C. elegans]"

Wang DY, Yu HX, Wang XY

[Huan Jing Ke Xue, 2007]

Model organism C. elegans was introduced into the toxicity identification evaluation (TIE) procedure to determine the toxification of acid and alkalescent industry liquid wastes from paper mill via analyzing the life span, days corresponding to 50% lethal, reproductive rate, reproductive capacity and locomotion behaviors in worms. Our data indicate that the toxic influence order of different samples on life span was: 2nd > 4th > 1st > 3rd, the toxic influence order of different samples on generation time was: 2nd > 1st > 3rd approximately 4th, the toxic influence order of different samples on brood size and head thrashes was similar: 1st approximately 2nd > 3rd approximately 4th, the toxic influence order of different samples on body bends was: 1st approximately 2nd > 3rd > 4th, and the 2nd sample always had more significantly toxic effects on different phenotypes. Thus, the toxicity of acid industry liquid waste from the paper mill relatively had the most severe multiple effects, whereas the toxicity of alkalescent industry liquid and grain-like wastes only exhibited limited effects.

Infarinato N (2019) J Cell Biol "Xiaochen Wang: Building up our understanding of breaking down."

Infarinato N

[J Cell Biol, 2019]

Wang studies lysosomal degradation pathways using <i>C. elegans</i> as a model system.

Ye YX et al. (2021) Analyst "A novel fast-response and highly selective AIEgen fluorescent probe for visualizing peroxynitrite in living cells, C. elegans and inflammatory mice."

Chen XY, Wang BZ, Zhang Q, Yu YW, Wang ZC, Wei XW, Ye YX, Zhu HL, Jiao QC

[Analyst, 2021]

Most of the ONOO- fluorescent probes have restricted applications because of their aggregation-caused quenching (ACQ) effect, long response time and low fluorescence enhancement. Herein, we developed a novel AIEgen fluorescent probe (PE-XY) based on a benzothiazole and quinolin scaffold with high sensitivity and selectivity for imaging of ONOO-. The results indicated that probe PE-XY exhibited fast response towards ONOO- with 2000-fold enhancement of fluorescence intensity ratio in vitro. Moreover, PE-XY exhibited a relatively high sensitivity (limit of detection: 8.58 nM), rapid response (<50 s), high fluorescence quantum yield (delta = 0.81) and excellent selectivity over other analytes towards ONOO-in vitro. Furthermore, PE-XY was successfully applied to detect endogenous ONOO- levels in living HeLa cells, C. elegans and inflammatory mice with low cytotoxicity. Overall, this work provided a novel fast-response and highly selective AIEgen fluorescent probe for real-time monitoring ONOO- fluctuations in living systems.

Wang JT et al. (2014) Curr Biol "P granules."

Seydoux G, Wang JT

[Curr Biol, 2014]

Wang and Seydoux discuss the functional importance of P granules - the germline-specific RNA granules of C. elegans.

Post R (2005) Filaria J "The chromosomes of the Filariae."

Post R

[Filaria J, 2005]

An understanding of the nature of the chromosomes of the filariae is expected to greatly assist the future interpretation of genome data. Filarial development is not eutelic, and there does not seem to be a fixed number of cell divisions in the way that there is in Caenorhabditis. It is not clear whether the chromosomes of the filariae have localized centromeres or whether they are holocentric. Sex determination is by a chromosomal &quot;balance&quot; X0 system in most filariae, but in some Onchocercidae there has been a chromosomal fusion to create a neo-XY system. It is presumed that the molecular basis of sex determination in filariae is similar to Caenorhabditis. The ancestral karyotype of the filariae is probably 5A+X0, but in some Onchocercidae this has been reduced to 4A+XY, and in O. volvulus and O. gibsoni it has been further reduced to 3A+XY. Onchocerca volvulus and O. gibsoni both have supernumary (B-) chromosomes and in O. volvulus there is a single active nucleolus organising region near the middle of the long autosome.

Munro E (2017) Dev Cell "Protein Clustering Shapes Polarity Protein Gradients."

Munro E

[Dev Cell, 2017]

In this issue of Developmental Cell, Dickinson etal. (2017) and Rodriguez etal. (2017), along with Wang etal. (2017) in Nature Cell Biology, show how PAR protein oligomerization can dynamically couple protein diffusion and transport by cortical flow to control kinase activity gradients and polarity in the C.elegans zygote.

Rashid S et al. (2017) Dev Cell "Packing on the Pounds in Response to Bacterial Growth Conditions."

Rashid S, MacNeil LT

[Dev Cell, 2017]

Reporting in Nature Cell Biology, Lin and Wang (2017) show that bacterial methyl metabolism impacts host mitochondrial dynamics and lipid storage in C.elegans. The authors propose a model whereby bacterial metabolic products regulate a nuclear hormone receptor that promotes lipid accumulation through expression of a secreted Hedgehog-like protein.

Vincenz L et al. (2014) Cell "Sugarcoating ER Stress."

Vincenz L, Hartl FU

[Cell, 2014]

The hexosamine biosynthetic pathway (HBP) generates metabolites for protein N- and O-glycosylation. Wang et al. and Denzel et al. report a hitherto unknown link between the HBP and stress in the endoplasmic reticulum. These studies establish the HBP as a critical component of the cellular machinery of protein homeostasis.

Sakaguchi Y et al. (1983) J Parasitol "Karyotypes of Brugia pahangi and Brugia malayi (Nematoda: Filarioidea)."

Sakaguchi Y, Ash LR, Tada I, Aoki Y

[J Parasitol, 1983]

Using air-dried preparations of the testis and ovary, karyotypes were analyzed and compared to each other in two species of filarial parasites, Brugia pahangi and B. malayi. Both species had a diploid number of 10 chromosomes and were karyotypically very similar. C-banding analyses disclosed that the sex-determining mechanism of these species was of the XY-XX type, where the X chromosome was the largest, and the Y chromosome was of medium-size.