[
International Worm Meeting,
2021]
To exercise information about its immediate surroundings C.elegans receive a variety of external stimuli, which establishes behavioral modifications in animals. Monoamines seem to mediate a wide range such functions along with physiological and homeostatic functions in animals. Recent evidences suggest Tyramine (TA), one of the precursors for monoamines like dopamine and serotonin, could act as a neurotransmitter. However, the molecular mechanisms underlying the tyramine pathway is poorly understood. Tyraminergic neurons, expressing SER-2 receptors, could act as neuromodulator and facilitate escape response in C. elegans. Yet another receptor TYRA-2 acts as determining factor in shut down of aversive behaviours in unharmonious situations for Risk-Reward pathway.Tyramine secretion being extrasynaptic from Motor interneuron RIM is likely to affect the head neurons, presenting receptors towards tyramine.This mechanism might be credited in establishing essential behavioral modifications and short lived neuronal alterations in the animals. In this study, we verified the role of monoamines by c.elegans behaviour using olfactory learning and memory generation. A significant increase in memory was found in worms on providing tyramine extra-synaptically. Mechano-stimulation assay in presence of tyramine also correlates with behavioral alterations via omega turns and reversal of worm, suggesting escape response activity coordinated with motor neuronal stimulation (RIM). These results suggest that the monoaminergic molecular modulation in associated neurons facilitates alterations in memory formations. The top down approach of multisensory decision involving ASH and AWA is advocated by tyramine, this can also possibly alter preferences based on learning and memory in worms. Key words- Tyramine, SER-2, TYRA-2, Mechano-stimulation, Associative memory