Braukmann F et al. (2017) RNA Biol "Artificial and natural RNA interactions between bacteria and C. elegans."

Jordan D, Miska E, Braukmann F

[RNA Biol, 2017]

19years after Lisa Timmons and Andy Fire first described RNA transfer from bacteria to C. elegans in an experimental setting [Timmons and Fire, 1998 ] the biological role of this trans-kingdom RNA-based communication remains unknown. Here we summarize our current understanding on the mechanism and potential role of such social RNA.

Levenson MT et al. (2023) STAR Protoc "Protocol for nuclear dissociation of the adult C. elegans for single-nucleus RNA sequencing and its application for mapping environmental responses."

Panter B, Allard P, Barrere-Cain R, Reich E, Yang X, Levenson MT, Truong L, Shuck K, Chen YW

[STAR Protoc, 2023]

Caenorhabditis elegans is a valuable model to study organ, tissue, and cell-type responses to external cues. However, the nematode comprises multiple syncytial tissues with spatial coordinates corresponding to distinct nuclear transcriptomes. Here, we present a single-nucleus RNA sequencing (snRNA-seq) protocol that aims to overcome difficulties encountered with single-cell RNA sequencing in C. elegans. We describe steps for isolating C. elegans nuclei for downstream applications including snRNA-seq applied to the context of alcohol exposure. For complete details on the use and execution of this protocol, please refer to Truong et al. (2023).¹.