Toraason E et al. (2021) STAR Protoc "Detection of homolog-independent meiotic DNA repair events in C.elegans with the intersister/intrachromatid repair assay."

Toraason E, Libuda DE, Glover M, Horacek A

[STAR Protoc, 2021]

Accurate repair of DNA double-strand breaks (DSBs) in developing germ cells is critical to promote proper chromosome segregation and to maintain genome integrity. To directly detect homolog-independent (intersister/intrachromatid) meiotic DSB repair, we exploited the genetics and germline physiology of C.elegans to (1) induce a single DSB in nuclei across discrete stages of meiotic prophase I; (2) detect repair of that DSB as a homolog-independent crossover or noncrossover; and (3) sequence the resultant product to assess mechanisms of recombination. For complete details on the use and execution of this protocol, please refer to Toraason etal. (2021).