Vladimir V Bakaev et al. (2003) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 32. No positive effect of peroxide"

Vladimir V Bakaev, Lyudmila M Bakaeva

[Worm Breeder's Gazette, 2003]

The purpose of this study was to investigate the effect of hydrogen peroxide in water solutions on the nematode life span. In this experiment hydrogen peroxide was used in following dilutions: 100 mg/L, 10 mg/L, 1 mg/L, 0.1 mg/L, 0.01 mg/L and 0.001 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without hydrogen peroxide) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without hydrogen peroxide in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with hydrogen peroxide in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Conclusion: If hydrogen peroxide solution was applied to C. elegans, it was not able to increase their mean longevity significantly in comparison with control.

Vladimir V Bakaev et al. (2003) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 31. No positive effect of glycerol"

Lyudmila M Bakaeva, Vladimir V Bakaev

[Worm Breeder's Gazette, 2003]

The purpose of this study was to investigate the effect of glycerol in water solutions on the nematode life span. In this experiment glycerol was used in following dilutions: 100 g/L, 10 g/L, 1 g/L, 100 mg/L, 10 mg/L, 1 mg/L and 0.1 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without glycerol) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without glycerol in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with glycerol in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.

Vladimir V Bakaev et al. (2003) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 30. No positive effect of kudesan"

Lyudmila M Bakaeva, Vladimir V Bakaev

[Worm Breeder's Gazette, 2003]

The purpose of this study was to investigate the effect of kudesan (a water-soluble medicine, containing 30.0 mg of ubiquinone and 4.5 mg of a-tocopherol in 1 mL) in water solutions on the nematode life span. In this experiment kudesan was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without kudesan) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without kudesan in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with kudesan in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.

Rosenberg R (1978) Worm Breeder's Gazette

Rosenberg R

[Worm Breeder's Gazette, 1978]

All eyes are on the newest fashion trend, the Dumpy Look . Pace setting designer I.M. Worm s androgynous wardrobe is all the rage in Paris. Bianca Jagger quips, Tres, tres - Women s Wear Daily writes, Elegans personified - Patti Smith thinks, The punks won t buy it and Craig Russell says, It fits right in with my act . A product of Mutant Isolation, Inc.

Golden JW (1980) Worm Breeder's Gazette "C elegans dauer larvae, and cyclic Amp."

Golden JW

[Worm Breeder's Gazette, 1980]

Cyclic AMP levels have been assayed in N2 synchronous cultures and starvation-induced dauer larvae as a first step in the analysis of hormonal involvement in dauer larva formation, maintenance, and recovery. N2 worms are synchronized by egg purification (after the method of Ralph Hecht), hatching into M9 buffer, and resuspension in liquid worm medium containing an adenyl cylase deletion mutant of E. coli. The worms are harvested at various time intervals by immediately microwaving to boiling and washing in glass distilled water. This is followed by lyophillization after which the dry weight is determined. The assay uses a cAMP binding protein (Calbiochem) in a competitive binding assay with [3H]cAMP. After equilibrium is reached, the protein is filtered through a nitrocellulose filter and counted in a liquid scintillation counter. Initial results indicate that changes in cAMP concentration are correlated with the life cycle. Assays performed on well fed worms at various times in larval development show a linear decrease in cAMP levels from 25 pmol/mg dry weight in L2's to 15 pmol/mg dry weight in L3's, down to 1 pmol/mg dry weight in L4's. Starvation-induced dauer larvae have a cAMP level of 5 pmol/mg dry weight, or about one-third the level present at the L2 molt. Recovering dauers isolated 6 hours after the addition of food to starvation-induced dauers, show a dramatic drop in cAMP levels to less than 0.1 pmol/mg dry weight. The data collected to this point indicate that dauer larvae have a reduced level of cAMP compared to the L2 an' L3 larval stages and that recovery from the dauer stage results in cAMP levels below that found in L4's. These preliminary studies are to be followed by a more detailed study of cAMP and cGMP levels in N2 worms at all stages of the life cycle and in selected dauer-constitutive mutants. Cyclic GMP assays and pharmacological studies using theophylline as a phosphodiesterase inhibitor are currently being developed.

Starck J (1976) Worm Breeder's Gazette "RNA synthesis during oogenesis of C. elegans bergerac."

Starck J

[Worm Breeder's Gazette, 1976]

RNA synthesis has been studied during oogenesis by autoradiography. Allow incorporation to occur, ovary has been dissected out of gravid adults into Mg medium containing 3H uridine. RNA is synthesized actively until the end of meiotic prophase. This synthesis follows a classical model : label appears at first in the nucleus and after in the cytoplasm. This 'in vitro system' gives homogeneous results in each sample .

Cassada RC et al. (1980) Worm Breeder's Gazette "initial characterization of ts embryonic defective (emb) mutants (Goettingen set)."

Culotti MR, Cassada RC, Von Ehrenstein G, Isnenghi E

[Worm Breeder's Gazette, 1980]

[See Figure 1] Already published allelism: Wood et al., 1980: b117=b189; b246 = let- 2.Miwa et al., 1980: hc57 = hc62; hc61 = hc67. Our global complementation results: g1 = g4; g16 = g65 = hc61 = hc67; g36 = hc65; g23 = g34 = hc70 = b117 = b189; g37 = b246 = let-2; b84 = hc66; g14 = g43; g57 = b1O. From frequency of multiple alleles we estimate 200-400 genes essential for embryogenesis. Mapping is in progress. We are also doing tsp's, R + H Tests, and cellular defects ( Isnenghi, Cassada, Radnia, Schierenberg, K. Smith, v. Ehrenstein).

Vladimir V Bakaev et al. (2002) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 27. A positive effect of acetylsalicylic acid"

Alexey V Shabalin, Lyudmila M Bakaeva, Vladimir V Bakaev

[Worm Breeder's Gazette, 2002]

The purpose of this study was to investigate the effect of acetylsalicylic acid in water solutions on the nematode life span. In this experiment acetylsalicylic acid was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without acetylsalicylic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without acetylsalicylic acid in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with acetylsalicylic acid in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Conclusion: If acetylsalicylic acid solution was applied to C. elegans in concentration of 10 mg/L, it was able to increase significantly (P>0.05) their mean longevity in comparison with control to 83.96 percent.

Coles GC et al. (1980) Worm Breeder's Gazette "Use of C. elegans for drug detection"

Simpkin KG, Coles GC

[Worm Breeder's Gazette, 1980]

By maintaining C. elegans on ampicillin treated E. coli a high throughput inexpensive screen for detecting potential anthelmintics is obtained. Method: 5 mg of ampicillin and 1.7 mg nystatin are added to 10 mls of E. coli broth (Uracil requiring mutant) and 100 mls of M9 buffer and the mixture stood for 2 hrs. Multi well plates are filled with 2 mls of the mixture and substances to be tested are added in the minimum volume of water, ethanol or DMSO. Between 12 and 20 worms of mixed age are added per well and after 7 days incubation at 20 C experimental wells are compared to controls for numbers of larvae and adults and normal movement. Most benzimidazoles are detected at 1 p.p.m. with the exceptions of oxfendazole (10 p.p.m.) and thiabendazole (2.5 p.p.m.). Levamisole is detected down to 0.5 p.p.m. and avermectin Bla to 0.0005 p.p.m. At 50 p.p.m. less than 10% of random compounds are active. Although older drugs such as piperazine , bephenium, diethylcarbamazine, methyridine and santonin would be missed this is not considered to be of significance as modern drugs must be both broadspectrum and very active. The test can also probably be used to detect potential plant nematocides.

Dew M et al. (1975) Worm Breeder's Gazette "DNase"

Sulston JE, Dew M

[Worm Breeder's Gazette, 1975]

E1392 (nuc-1,X) lacks the principal endodeoxyribonuclease. When determined on crude sonicates by an assay sensitive to endolytic cleavage of DNA (e.g. the spectrophotometric method of Kunitz, reviewed in Prog. in Nucl. Acid Res. 1 85 (ed Cantoni and Davies, 1967)), the DNase activity is well under 1% of that in N2. Feulgen staining reveals stranded material in the gut, persistent DNA from programmed cell deaths, and extruded polar bodies accumulating in the gonads of old animals; this suggests that a single endo-DNase is used both in the gut and in the tissues of C. elegans. There is no visible phenotype. The map position is: [See Figure 1] The endo-DNase of N2 is equally active in the presence of Mg++ and of EDTA