[
Curr Opin Neurobiol,
2011]
The presynaptic active zone (AZ) membrane is the site where vesicle fusion mediates information transfer between connected neurons. Reaching into the cytoplasm, an electron-dense cytomatrix (CAZ) is found to decorate the AZ membranes. CAZ architectures are meant not only to regulate the synaptic vesicle exocycle/endocycle, but also to structurally stabilize the presynaptic site. The CAZ is composed of a set of large scaffold proteins, many of which are evolutionarily conserved. Recently, several signaling factors controlling the developmental assembly of CAZs were found by unbiased genetics in Drosophila and Caenorhabditis elegans. At the same time, post-translational modification of CAZ proteins was implicated in changing the strength of mammalian brain synapses. Studying how processes of structural and functional CAZ plasticity get integrated within circuit remodeling remains an important challenge.
[
Curr Opin Neurobiol,
2009]
Rapid neurotransmission depends on the structural and functional integrity of synaptic connections. How synapses assemble is currently being intensely investigated to help our understanding of neuronal development and synaptic plasticity. Here we focus on the assembly of the presynaptic active zone, which regulates the synaptic vesicle exo/endo-cycle and is characterized by ultrastructural specializations and large scaffold proteins. While genetic and biochemical studies from rodents, Caenorhabditis elegans and Drosophila have started to identify proteins organizing active zone assembly, drawing a coherent picture remains challenging, with genetically established hierarchies and protein-protein interactions still to be placed into spatio-temporal and functional context. Recent advances in light and electron microscopy, together with in vivo imaging of protein traffic, will help to tackle this challenge.