[
STAR Protoc,
2021]
This protocol describes the culturing of the nematode <i>Caenorhabditis elegans (C. elegans)</i> in a sterol-defined experimental system and the subsequent quantitative analysis of <i>C.elegans</i> sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. <i>C.elegans</i> cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, <i>C.elegans</i> is a powerful system tostudy metabolism in sterol-defined conditions that are described in our protocol. For complete details on the use and execution of this protocol, please refer to Shamsuzzama etal. (2020).