Colonnello A et al. (2020) Neurotox Res "Correction to: Comparing the Neuroprotective Effects of Caffeic Acid in Rat Cortical Slices and Caenorhabditis elegans: Involvement of Nrf2 and SKN-1 Signaling Pathways."

Tunez I, Rubio-Lopez LC, Aguilera-Portillo G, Silva-Palacios A, Evaristo-Priego Y, Santamaria A, Galvan-Arzate S, Cortez-Nunez S, Aschner M, Rangel-Lopez E, Chen P, Colonnello A, Robles-Banuelos B, Garcia-Contreras R

[Neurotox Res, 2020]

One of the authors has incorrect family name spelling in the original article_. Michael Ashner should read as Michael Aschner.

de Groot-Andralojc, Karolina et al. (2015) International Worm Meeting "Identification of novel genes regulating P-granule function in C. elegans."

King, Benjamin, Terrey, Markus, Senter-Zapata, Michael, de Groot-Andralojc, Karolina, Campbell, Anne, Updike, Dustin

[International Worm Meeting, 2015]

P granules in C. elegans are associated with RNA metabolism and function to maintain the integrity of germ cells. We previously published a genome-wide RNAi screen for aberrant P granules, where we found that depletion of the gene encoding CSR-1, as well as its cofactors DRH-3 and EGO-1, causes a very distinct large P-granule phenotype. CSR-1 is an Argonaute protein and a core component of P granules that acts to suppress the extent of sperm-gene expression in the hermaphrodite germline. Our aim was to identify additional genes that are essential for P-granule function and proper germ cell development.To address this, we performed an EMS mutagenesis in a PGL-1::GFP strain and used fluorescence microscopy to screen 4000 haploid genomes for large and misshaped P-granules. From this screen we isolated ten alleles that fall into six complementation groups. Two of these alleles, sam15 and sam18, contain mutations in the PAZ domain of csr-1 itself, validating our approach. We have stained and quantified the expression of endogenous P-granule components in each of our mutants to show that their large P granules are independent of the PGL-1::GFP transgene. We next used a Hawaiian Variant Mapping strategy and the CloudMap pipeline to clone a number of these alleles, revealing known CSR-1 cofactors but also novel components required for P-granule assembly. CRISPR technology, together with RNAi and fosmid microinjections were used to validate whether identified mutations are linked to observed phenotypes. Here we report on a novel gene with early stop codons in sam3 and sam6 alleles that alters P-granule appearance and expression, but is not required for fertility. Single-molecule FISH shows that this gene is enriched and expressed through the adult germline. We are currently investigating how various alleles of the novel gene affect P-granule morphology and function. Our results broaden our understanding of the role of germ granules in the germline as well as the epigenetic mechanisms that regulate germ cell integrity.

Hengartner MO et al. (1992) Worm Breeder's Gazette "unc-50, a Gene Required for Acetylcholine Receptor Function, Encodes an Unfamiliar Protein"

Horvitz HR, Tsung N, Hengartner MO, Lewis JA

[Worm Breeder's Gazette, 1992]

unc-50, a gene requiired for acetylcholine receptor function, encodes an unfamiliar protein Michael Hengartner, Nancy Tsung, Jim Lewist, and Bob Horvitz HHMI, Dept. Biology, MIT, Cambridge, MA 02139, USA. t Division of Life Sciences, U. of Texas at San Antonio, San Antonio, IX 78249

Holway AH et al. (2007) J Cell Biol "Checkpoint silencing during the DNA damage response in Caenorhabditis elegans embryos"

Kim SH, Michael WM, La Volpe A, Holway AH

[J Cell Biol, 2007]

After publication of this manuscript, the authors noticed that two images (Fig. 4 B, top left and middle) had been duplicated from a previous publication (Holway, A.H., C. Hung, and W.M. Michael. 2005. Genetics. 169:14511460). Here we provide new images corresponding to these control experiments. The authors apologize for this oversight.

Zhou MH et al. (1996) Worm Breeder's Gazette "Liquid Culture of the Worms with a Fermentor"

Zhou MH, Yang H, Walthall WW

[Worm Breeder's Gazette, 1996]

The yield of C. elegans and their food (E.coli) are relatively low in the large liquid cultures growth (Koelle et al.,1994) and the whole growth process is tedious. Here we report the successful growth of C.elegans with a fermentor. The following protocol is based on the protocol by Michael Koelle and Tory Herman (1994) from Internet.

Michael WM (2017) MicroPubl Biol "Asymmetric distribution of cyb-3 in 4-cell stage embryos."

Michael WM

[MicroPubl Biol, 2017]

Early embryos were fixed and stained with Mab F2F4 (green), shown to recognize CYB-3 (Michael, 2016), and DAPI, to illuminate the DNA (blue). Either wild type or par-4 mutant embryos were examined, after 24-hour incubation at 25C (the non-permissive temperature for the it47 allele of par-4). Anterior is to the left in all images. The data presented here reveals previously not shown data that depicts CYB-3 as asymmetrically distributed at the 4-cell stage. These data further support reported findings in Michael, 2016. There is more CYB-3 in the AB cell relative to its sister P1. In 4-cell embryos there is more CYB-3 in the EMS cell relative to its sister, P2. Thus, during P-lineage divisions, CYB-3 is asymmetrically distributed such that the somatic precursor receives more than its germline precursor sister cell. This asymmetry is abolished in par-4 mutant embryos, where all blastomeres contain equivalent amounts of CYB-3.

Vairoletti F et al. (2019) Medchemcomm "Synthesis of bicyclic 1,4-thiazepines as novel anti-Trypanosoma brucei brucei agents."

Medeiros A, Fontan P, Jancik V, Melendrez J, Saiz C, Vairoletti F, Tabarez C, Mahler G, Franco J, Salinas G, Comini MA, Saldana J

[Medchemcomm, 2019]

1,4-Thiazepines derivatives are pharmacologically important heterocycles with different applications in medicinal chemistry. In the present work, we describe the preparation of new bicyclic thiazolidinyl-1,4-thiazepines <b>3</b> by reaction between azadithiane compounds and Michael acceptors. The reaction scope was explored and the yields were optimized. The activity of the new compounds was evaluated against <i>Nippostrongylus brasiliensis</i> and <i>Caenorhabditis elegans</i> as anthelmintic models and <i>Trypanosoma brucei brucei.</i> The most active compound was <b>3l</b>, showing an EC₅₀ = 2.8 +/- 0.7 M against <i>T. b. brucei</i> and a selectivity index &gt;71.

Kron M et al. (2000) Parasitol Today "Lymphatic filariasis in the Philippines."

Kron M, Libranda-Ramirez B, Hernandez L, Torres E, Walker E

[Parasitol Today, 2000]

Lymphatic filariasis caused by Wuchereria bancrofti and Brugia malayi is endemic throughout most of the southern half of the Philippine archipelago. Economic and manpower shortages prior to 1996 made it difficult to acquire new prevalence data and vector control data concurrently from all provinces. Nevertheless, analysis of cumulative prevalence data on filariasis indicates the persistence of filariasis in each of the three major island groups - Luzon, Visayas and Mindanao - including 45 out of 77 provinces. Here, Michael Kron and colleagues summarize the prevalence data, and review host, parasite and vector characteristics relevant to the design and implementation of disease control initiatives in the Philippines planned for the year 2000.

Mango S (2009) Curr Biol "Q&A: Susan Mango."

Mango S

[Curr Biol, 2009]

Susan Mango is Benning Professor of Oncological Sciences at the University of Utah and an Investigator at the Huntsman Cancer Institute. She grew up in England and Washington D.C. before attending Harvard University. She received her Ph.D. from Princeton, where she studied the c-myc oncogene with Michael Cole. She was introduced to the nematode Caenorhabditis elegans as a postdoc with Judith Kimble at the University of Wisconsin - Madison, and moved to Utah in 1996 to start her own lab. After 13 years at the University of Utah, she will move to Harvard University in July 2009. Her principal focus is transcriptional strategies of organ development, using the C. elegans foregut as a model.

Roussell DL et al. (1994) Parasitol Today "Germ-line determination in Caenorhabditis and Ascaris: will a helicase begin to unravel the mystery?"

Bennett KL, Roussell DL, Gruidl ME

[Parasitol Today, 1994]

How cell lineages are established during development in higher eukaryotes is being addressed by geneticists and by developmental and molecular biologists. In Drosophila melanogaster, a gene corresponding to a germ-line-specific RNA helicase, vasa, has been shown to be a component o f the posteriorly localized germ granules o f the developing embryo. A putative RNA helicase, glh-I r which appears germ-line specific in its expression, has recently been reported from the free-living nematode Caenorhabditis elegans. Parasitologists studying the nematode Ascaris lumbricoides var. suum have found it to be a useful complement to Caenorhabditis. Deborah Roussell, Michael Gruidl and Karen Bennett predict that Ascaris will be valuable in determining the role played by germ-line helicases in development.