Matsumura, Yasuki, Matsumoto, Shinya, Morishita, Mayu, Sei, Megumi, Ogai, Midori, Kato, Kosuke, Saito, Haruka, Nishida, Ayano, Sawanaga, Akari, Okada, Sawako, Sakagami, Midori
[
International Worm Meeting,
2015]
According to the DOHaD (Developmental Origins of Health and Disease) concept, poor nutritional environment during early life (including conception, embryogenesis, infancy and early childhood) induces changes in development that increase the disease risk in adult stage. The DOHaD is attracting many attention because not only it could change our view toward health and diseases, but also it causes concern about the health of our future generation. Generally, the three molecular events, DNA methylation, histone modifications, and non-coding RNAs are considered to be involved in DOHaD process in vertebrates, but the precise mechanism is not well defined. To gain information concerning the molecular mechanism of DOHaD, starvation was introduced to the parent worms, and the offsprings were analyzed for their fecundity, life span, mobility and fat accumulation. There were no difference observed in fecundity, life span and mobility between the offsprings derived from starvation-experienced and fed-parent worms. However, Nile Red staining showed that fat content was significantly increased in the offsprings derived from starvation-experienced worms compared with that of control offsprings. Fat accumulation was further indicated by gas chromatography analysis. DNA microarray is now being conducted to analyse the gene expression pattern.The data is in line with DOHaD concept, which may indicate that C.elegans can be used in DOHaD model animal.