Pinan-Lucarre, Berangere et al. (2009) International Worm Meeting "Transgenic C. elegans expressing the fungal prion protein HET-s as a model for the study of amyloid infectivity and toxicity."

Qiao, Yujie, Saupe, Sven, Pinan-Lucarre, Berangere, Driscoll, Monica

[International Worm Meeting, 2009]

Amyloids are protein fibers rich in beta sheet structure that are associated with numerous neurodegenerative diseases, including Alzheimer disease. Amyloids have two major biological properties. First, they can be infectious--meaning that they can spread the altered amyloid conformation to the same protein or even among proteins of distinct primary sequence (the latter phenomenon is known as cross-polymerization). Infectious amyloids are called prions. Second, amyloids can be toxic, whether they are infectious or not. Both properties have tremendous fundamental and biomedical impact. [Het-s] is a prion of the fungus Podospora anserina involved in a defense cell suicide mechanism named heterokaryon incompatibility, which disables mixing of different strains by somatic fusion, an ubiquitous feature in filamentous fungi. The HET-s protein exists in a soluble state or in an aggregated, prion state named [Het-s]. The [Het-s] prion is not toxic by itself in P. anserina or in yeast. However cell death is rapidly triggered by the lethal interaction between 2 alleles of the het-s locus: het-S (het-"large S") and het-s (het-"small s") when the HET-s protein adopts the prion form. This prion has been extensively characterized and it is the only prion for which tridimensional structure is available to date. The critical point is that in the [Het-s] system, transgenic expression of het-s allows propagating a non-toxic prion while co-expression of het-s and het-S generates toxicity. We constructed strains expressing het-s or the prion domain only het-s(218-289) (the 218-289 fragment of the protein) as GFP fusions under the control of the ubiquitous promoter dpy-30. The fluorescence of pdpy-30het-s::gfp appears to be mainly diffuse in the cytoplasm while it shows strong aggregation in pdpy-30het-s(218-289)::gfp strains. We constructed strains expressing het-s(218-289)::gfp in the touch neurons using mec-4 promoter and observed that the fusion protein is mostly aggregated into a large fiber spanning the cell body and the proximal part of the axon. These aggregates remain to be shown as infectious [Het-s] amyloids. In future experiments, we will aim to generate neuronal toxicity through the co-expression of het-s(218-289) and het-S. This C. elegans model for prion studies, filling a gap between simple fungal systems and highly complex mammalian systems, might allow a better understanding of the molecular and cellular basis of amyloid infectivity and toxicity.

Dana L. Miller et al. (2007) International Worm Meeting "Adaptation to hydrogen sulfide increases thermotolerance and lifespan."

Mark B. Roth, Dana L. Miller

[International Worm Meeting, 2007]

Hydrogen sulfide (H₂S), which is naturally produced in animal cells, has been shown to effect physiological changes that improve the capacity of mammals to survive environmental changes. We have investigated the physiological response of C. elegans to H₂S to begin to elucidate the molecular mechanisms of H₂S action. We show that nematodes exposed to H₂S are apparently healthy and do not exhibit phenotypes consistent with metabolic inhibition. However, we observed that animals exposed to H₂S had increased thermotolerance and lifespan and survived subsequent exposure to otherwise lethal concentrations of H₂S. Increased thermotolerance and lifespan is not observed in the sir-2.1(ok434) deletion mutant exposed to H₂S. However, mutants in the insulin signaling pathway (both daf-2 and daf-16), animals with mitochondrial dysfunction (isp-1 and clk-1) and a genetic model of caloric restriction (eat-2) all exhibit H₂S-induced increased thermotolerance. These data suggest that H₂S activates a pathway including SIR-2.1 that is separate from dietary restriction and insulin signaling that results in increased lifespan. Moreover, these studies suggest that SIR-2.1 activity may translate environmental change into physiological alterations that improve survival. It is interesting to consider the possibility that the mechanisms by which H₂S increases thermotolerance and lifespan in nematodes are conserved, and that studies using C. elegans may help explain beneficial effects observed in mammals exposed to H₂S.

Oppenheimer AJ et al. (1995) International C. elegans Meeting "G-PROTEIN SIGNALING IN C. ELEGANS"

Kaplan JM, Oppenheimer AJ

[International C. elegans Meeting, 1995]

We are interested in determining how G-proteins modulate the activity of ion channels in the C. elegans nervous system. To generate behavioral phenotypes dependent on G-protein signaling, we targeted expression of mammalian G-proteins to a set of neurons including those controlling foraging and locomotion (RMD, AVA, AVB, AVD, and PVC). Ectopic expression of several G-proteins under the control of the not-3 promoter results in behavioral defects. Expression of constitutively active rat G-alpha-i1 causes uncoordinated forward and backward locomotion. Expression of wild-type rat G-alpha-s impairs backward locomotion, while constitutively active rat G-alpha-s causes defective backward locomotion and lethargy. In order to identify components of the G-alpha-s signaling pathway, we have initiated a screen for mutations that suppress the behavioral effects of activated G-alpha-s expression. In a screen of approximately 7000 haploid genomes, we have identified 11 independent mutations that improve backward locomotion. Several of these mutations result in a hyperactive phenotype in the absence of activated G-alpha-s expression. Mapping and complementation tests will determine whether the suppressors are allelic to other hyperactive mutants isolated by D. Elkes and J. Kaplan. By cloning the suppressor genes, we hope to identify ion channels regulated by G-alpha-s as well as other components of the G-alpha-s signaling pathway. We also plan to determine whether the behavioral effects of G-alpha-s expression are mediated by known second messenger systems. We are first testing the role of cAMP-dependent protein kinase (PKA), because it is activated by G-alpha-s in other systems and has been shown to phosphorylate ion channels. If G-alpha-s signals through PKA, increasing the level of PKA activity should mimic the effect of activated G-alpha-s expression, and inhibiting PKA should suppress the effect of activated G- alpha-s expression.

Cundall M et al. (2006) European Worm Meeting "A Partial Loss of Function Allele of klp-19 Causes Intestinal Nuclear Division Defects and Sterility."

Johnstone IL, Clucas C, Cundall M

[European Worm Meeting, 2006]

Chromosomes must be replicated and segregated accurately to ensure the successful completion of mitosis. During embryonic development of the C. elegans E cell lineage 20 daughter cells are derived from a single progenitor cell to form the intestine. Post-embryonically there are no further cell divisions in the intestine,although manyadult intestinal cells are bi-nucleate as a result of nuclear division without cytokinesis that occurs atthe end of the L1 larval stage. ij51 is a recessive allele that was isolated from a forward genetic screen for mutants with an altered E-lineage (Clucas and Johnstone, 1999). The L1 nuclear division fails in a variable number of intestinal cells in ij51 homozygotes, causing abnormal elongated mono-nuclei in normally bi-nucleate cells. ij51 homozygotes are sterile, producing few embryos that arrest before morphogenesis.. We have identified a missense mutation in klp-19, a member of the kinesin-4 family of chromokinesins, in ij51 mutants. KLP-19 is a plus-end directed microtubule motor that localizes to chromatin between kinetochores and generates antipoleward forces on chromosomes during meiosis, preventing merotelic chromosome attachments (Powers et al, 2004). We suggest that ij51 is a partial loss of function allele, as homozygous animals are viable unlike another allele, bn126, which causes homozygous animals to arrest at the L1 stage (Powers et al, 2004). Data will be presented on the further characterization of klp-19(ij51).

Oppenheimer AJ et al. (1996) East Coast Worm Meeting "G-alpha-s signaling in C. elegans"

Kaplan JM, Oppenheimer AJ

[East Coast Worm Meeting, 1996]

We are interested in determining how G-proteins modulate the activity of ion channels in the C. elegans nervous system. To generate phenotypes dependent on G-protein signaling, we expressed mammalian G-proteins under the control of the glr-1 promoter, which drives expression in a set of neurons including those controlling foraging and locomotion (RMD, AVA, AVB, AVD, and PVC). Expression of wild-type rat G-alpha-s impairs backward locomotion, while consitutively active rat G-alpha-s causes paralysis and neuronal degeneration in a subset of G-alpha-s-expressing cells, primarily the PVC and AVD neurons. Our model for the neurodegeneration is that activated G-alpha-s is misregulating an ion channel in AVD and PVC, causing osmotic imbalance and consequent swelling. Degenerating neurons appear enlarged and vacuolated, similar to those in worms carrying gain-of-function mutations in deg-1 and other degenerins. We tested whether activated G-alpha-s is killing cells by misregulating known ion channels. Loss-of-function mutations in deg-1, mec-6, unc-36, unc-2, and egl-19 were unable to suppress the degenerations, suggesting that G-alpha-s is not acting through these degenerins or calcium channels. Mutations in ced-3 and ced-4 do not prevent G-alpha-s-induced degenerations, indicating that G-alpha-s is not killing through apoptosis. To identify the components of the signaling pathway downstream of G-alpha-s, we screened for mutations that suppress the paralysis caused by activated G-alpha-s. In a screen of 7,760 haploid genomes, we identified 8 mutations that suppress paralysis and neurodegeneration and 8 mutations that suppress only paralysis. Five of the degeneration suppressors map to chromosome III and may be allelic. These mutations are semidominant, and several cause a hyperactive phenotype in the absence of activated G-alpha-s. Because hyperactivity also results from mutations in the Go signaling pathway, our suppressor mutations may identify a gene invovled in both the Gs and Go signaling pathways. We are continuing to map and characterize the suppressor genes.

Costi D. Sifri et al. (2007) International Worm Meeting "Immune evasion by staphylococcal exopolysaccharide during C. elegans infection."

Jakob Begun, Dietrich Mack, Costi D. Sifri, Jessica M. Gaiani, Frederick M. Ausubel, Holger Rohde, Stephen B. Calderwood

[International Worm Meeting, 2007]

We have previously shown that C. elegans can serve as a model host for the study of S. aureus pathogenesis and host innate immunity. Here we characterize nematode killing by S. epidermidis. C. elegans fed S. epidermidis survive 3-5 days, which is ~1 day longer than their lifespan on S. aureus. Inactivation of the ica operon (the biofilm exopolysaccharide [PIA] biosynthetic operon and Yersinia hms homolog) in S. epidermidis strain M10 greatly reduces nematode killing compared to the isogenic parental strain 9142 and a plasmid complemented mutant. In S. aureus, overproduction of PIA due to derepression of ica augments nematocidal activity. FITC-WGA labeling confirmed the presence of PIA within the digestive tracts of worms fed 9142 but not M10. Attachment of bacteria or bacterial matrix to the nematode cuticle was not observed. Nematodes briefly exposed to 9142 could not be rescued by transfer to M10, and worms fed dilutions of 9142 in M10 at ratios as low as 1:10e4, respectively, still die. Pulse/chase experiments using 9142, M10, and a second PIA-deficient strain, ATCC 12228, showed that durable colonization requires an intact ica operon. Disruption of N2 worms fed 1:100 9142/M10 mixtures for 20h confirmed significant enrichment of 9142 within the digestive tract of N2 but not sek-1 animals. Moreover, sek-1 and pmk-1 animals were found to be equally sensitive to M10- and 9142-mediated killing, in contrast to wild-type N2 worms. Several conclusions can be drawn from these studies. (a) S. epidermidis can infect and kill worms. (b) Disruption of PIA biosynthesis abrogates S. epidermidis virulence, while overproduction of PIA in S. aureus augments virulence. (c) PIA-producing S. epidermidis has a competitive advantage over PIA-deficient S. epidermidis within the nematode digestive tract. (d) Genetic analysis suggests that PIA may protect luminal staphylococci from SEK-1 p38 MAPK-regulated immune responses.

Zdraljevic, S. et al. (2015) International Worm Meeting "A powerful C. elegans resource for identifying the genetic determinants underlying complex traits."

Zdraljevic, S., Shimko, T. S., Rosenberg, S., Tanny, R. E., Andersen, E. C.

[International Worm Meeting, 2015]

Identifying the genetic determinants of complex traits depends on our ability to gather accurate phenotype data and to correlate these data to genetic differences present in a population. Furthermore, a large population of individuals is required to identify genomic regions that have subtle contributions to overall phenotypic differences. To uncover genomic loci that control complex traits, we have developed a high-throughput and high-accuracy phenotyping pipeline and generated a panel of 1,200 recombinant inbred Caenorhabditis elegans lines. The recombinant inbred collection was derived from a modified version of the standard laboratory strain N2 and the highly diverged wild strain CB4856. To abrogate the confounding pleiotropic effects of the laboratory-adapted N2 npr-1 allele, we replaced this allele with the CB4856 allele. Additionally, the N2 peel-1 gene was disrupted to eliminate the incompatibility locus. Our phenotyping pipeline relies on the COPAS BIOSORT large particle nematode sorter to accurately quantify various fitness related traits in a population of individuals, including animal size, fecundity, optical density, and pharyngeal pumping rate. Using this pipeline, we are able to phenotype 96 recombinant lines exposed to 24 different conditions in one week. Altogether, we exposed 359 recombinant inbred lines to 70 different conditions. This massive effort led to the identification of 550 unique quantitative trait loci (QTL) that explain phenotypic variation in response to 46 environmental perturbations, including chemotherapeutics, anthelmintics, heavy metals, pesticides, and different food sources and temperatures. We observe both complex and simple genetic architectures in response to different perturbations. For example, variation in response to the anthelmintic abamectin maps to six QTL that together account for 90% of the phenotypic variance that can be attributed to genetic factors. By contrast, a single QTL on chromosome V explains 55% of the phenotypic variation in response to the chemotherapeutic bleomycin. The confidence interval surrounding this peak contains five candidate genes, one of which is known to be involved in the DNA damage response. Though we have identified approximately 10 QTL with strikingly large effects, we expect to increase the number of QTL substantially using the full recombinant inbred line collection. This approach will expedite the discovery of causal genes contributing to phenotypic variation in response to therapeutics that can increase the likelihood that one day we will have individualized medicine.

Scholer, Lone V. et al. (2011) International Worm Meeting "Characterization of pathogenesis of S. aureus and involvement of checkpoint response in C. elegans."

Hansen, Frederik D, Scholer, Lone V., Fuursted, Kurt, Noerregaard, Steffen, Olsen, Anders

[International Worm Meeting, 2011]

Staphylococcus aureus (S. aureus) is a frequent colonizer of humans contributing to normal bacterial flora in nose, skin and mucosa. In addition, it is an important human pathogen causing various diseases ranging from superficial skin infections to life-threatening infections. Methicillin resistant S. aureus (MRSA) strains are rapidly spreading in the community (CA-MRSA) and is consequently becoming a more serious health threat. The capacity of S. aureus to cause this spectrum of human diseases reflects an ability to adapt to distinct microenvironments in the human body and suggests that the pathogenesis of S. aureus infection is a complex process involving a diverse array of virulence determinants that are coordinately expressed at different stages of infection. Studies have shown that virulence factors are very differently distributed among strains and are not always regulated in the same way, reflecting the ability of S. aureus to adapt and survive in different environmental niches and resist antibiotic treatment. C. elegans has previously been established as a model host for the study of S. aureus pathogenesis and CA-MRSA strains are capable of killing C. elegans[1]. We have screened thirty clinical MRSA isolates and found that in terms of pathogenicity they can be divided into separate classes. Using these classes we wish to identify new virulence markers and novel immunity pathways activated in response to S. aureus infection. We are particularly interested in evaluate virulence determinants during colonization versus infection and establishing their clinical relevance in humans. Inactivation of some S-M checkpoint proteins increase stress resistance and lifespan of C. elegans[2]. Since there may exist a potential correlation between stress response and immune function we are studying the role of checkpoint proteins to S. aureus exposure. We have identified several novel genes with potential checkpoint function which increase lifespan and thermotolerance. We find that that mutation of one of these, the transmembrane protein NDG-4, also confers resistance towards S. aureus infection. Epistasis analysis reveals that increased tolerance towards S. aureus infection is partially dependent on insulin signaling but that another unidentified component is involved as well. 1. Sifri, C.D., et al. 2003. 71(4): p. 2208-17. 2. Olsen, A., M.C. Vantipalli, and G.J. Lithgow. Science, 2006. 312(5778): p. 1381-5.

Jafa Armagost et al. (2007) International Worm Meeting "Investigating Potential Bacterial Sources of Dopaminergic Neurotoxicity in C. elegans."

Kim A. Caldwell, Guy A. Caldwell, Tyler Hodges, Jeana Blalock, Julie B. Olson, Jafa Armagost

[International Worm Meeting, 2007]

Parkinsons disease (PD) involves the progressive loss of dopamine (DA) neurons from the substantia nigra pars compacta (SNpc). Genetic forms of PD account for only 5-10% of known cases and environmental factors appear pivotal to sporadic causality. Evidence from both environmental and genetic studies of PD indicates that overloading the ubiquitin-proteasome system is a causative risk factor for PD. Furthermore, when proteasome inhibitors were injected directly into the brains of rats, PD-like symptoms resulted (McNaught et al., 2004; Ann Neurol. 56:149-162). Many proteasome inhibitors are isolated from bacterial strains within the order Actinomycetales, which are well known for the production of secondary metabolites. To determine if exposure to Actinomycetes could cause DA neurodegeneration in C. elegans, we exposed worms to three different species of Streptomyces (S. venezuelae, S. griseus, and S. coelicolor). When worms were grown on either S. griseus or S. coelicolor no DA neurodegeneration was observed. However worms exposed to S. venezuelae displayed DA neurodegeneration that increased over time. The causual factor is excreted by S. venezuelae, as conditioned media is sufficient for the neurodegenerative effect. This factor does not enter DA neurons through the DA transporter because degeneration is observed in dat-1 mutant animals. Further characterization of the S. venezuelae activity shows that DA degeneration does not result from generalized stress response or the unfolded protein response but may occur as a result of proteasome inhibition. Initial efforts to purify the factor have revealed that it is lipophilic and does not fit the molecular profile of known proteasome inhibitors.

Culotti JG et al. (1991) International C. elegans Meeting "THE UNC-S AXON GUIDANCE GENE ENCODES A PROTEIN WITH FEATURES OF A CELL ADHESION RECEPI OR."

Stern BD, Spence AM, Culotti JG, Scott IM, Hedgecock EM, Zhou YW, Leung-Hagesteijn CJ

[International C. elegans Meeting, 1991]

The unc-S gene is required to guide dorsal migrations of neuron growth cones and mesodermal cells on the nematode epidermis (Hedgecock et al., Neuron 4, 61-85, 1990). Genetically, unc-S requires unc-6 for its function and vice versa. One possibility is that unc-S encodes a receptor for the unc-6 product which has been shown to be a novel B2 laminin-like molecule (Ishii et al., WBG 10#3,39; Wadsworth et al., WBG 11#4,64). Several features of the predicted unc-S protein are consistent with this interpretation. The unc-S protein has a single membrane-spanning helix. Extracellular domains include one Ig-like and two tandem 'WSX' domains at the amino terminus. These domains are present in a variety of proteins that exhibit cell adhesion or recognition properties. The membrane-spanning domain has a pair of cysteines at its cytoplasmic end which are followed by a short run of basic amino acids. A similar motif is found in a number of G- proteinlinked receptors including rhodopsin and adrenergic receptors as well as in the nerve growth-associated protein GAP-43. In these proteins the cysteines are palmitoylated. This modification may be required for linkage to G-proteins. The predicted intracellular portion of unc-S comprises 557 amino acids. A 50 amino acid sequence within the cytoplasmic domain is distantly related to the SH3 domains of a variety of intracellular proteins associated with the membrane cytoskeleton, including several that bind to actin. An actin-binding function for this domain in unc-S could provide a direct mechanical linkage between the extracellular adhesive domains of unc-S and the actin-based motility system of migrating cells and growth cones. Experiments are underway to localize the unc-S protein and to determine whether it acts in migrating cells.