5 Papers found (0.008 seconds)

Wang C et al. (2018) Nat Commun "Author Correction: Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan."

[

Nat Commun,

2018]

The originally published version of this Article contained an error in the spelling of the author Nathaniel W. Oswald, which was incorrectly given as Nathaniel W. Olswald. This has now been corrected inboth the PDF and HTML versions of the Article.

Jiang, Karen et al. (2015) International Worm Meeting "Sensorimotor signal transmission through AIY interneuron in Caenorhabditis elegans during isothermal tracking."

Ling, Linsay, Mendoza, Steve, Sherry, Tim, Nowak, Nathaniel, Arisaka, Katsushi, Jiang, Karen, Haller, Leonard

[

International Worm Meeting,

2015]

Perception and navigation through space require accurate translation and transmission of sensory input to motor output. On a linear temperature gradient, Caenorhabditis elegans demonstrate a distinct behavioral phenotype in which they frequently travel along isotherms, maintaining sensitivity within 0.05 degC. This isothermal attractor state is correlated with movement at a constant and maximal velocity. We investigate how AIY, a first layer interneuron and postsynaptic partner to AFD thermosensory neuron, is able to integrate thermal information to return specific well-defined behavioral phenotypes. Prior observations of neural activity in vivo involve partial paralysis or constraint of the worm while stimuli is applied. Other systems circumvent this limitation by re-centering the stage; this generates an external force during stage acceleration introducing another stimulus. We overcome these two primary obstacles through the implementation of a novel automated worm-tracking epi-fluorescent microscope. The three-camera microscope system mounted on a movable XY stage captures dynamic Ca+2 signals in Cameleon-labeled neurons while the nematode navigates unconstrained along the temperature gradient. Implementing this set up, we observed that the greatest temperature difference occurs between the extremes of the head movement while along isotherms which phase lock with fluorescence response in AIY. The steady Ca+2 waveform in AIY suppresses reversals and maintains high speeds to downstream motor circuitry. .

Cole TS et al. (1975) Worm Breeder's Gazette "missing title ?"

Krieg C, Cole TS, Von Ehrenstein G, Schmitt D, Deppe U, Yoder B, Schierenberg E

[

Worm Breeder's Gazette,

1975]

This group works on C. elegans for about two years. Our main interest is the development of this organism. We are serial sectioning eggs in various cleavage stages to trace the cell lineage. Completed so far: 1 egg, 2 two-cell stages, 1 four- cell stage, 1 bretzel-stage. An assembly line is being organized and hopefully the turnout will speed up. Nomarski studies on cleaving eggs supplement the EM studies. A detailed study of the ultrastructure of the cleavage furrow and the formation of cell membranes at early cleavage stages has been initiated. We are reconstructing 3-D pictures using a PDP 11/45 computer with a Vector General Graphics Display. Work has been started to accumulate a set of cleavage-deficient mutants for morphological analysis. In addition some preliminary experiments show that eggs can be opened by treatment with a variety of proteolytic enzymes. In this way suspensions of embryonal cells can be obtained. Ruth Pertel is spending 4 months as a guest in the lab to teach us axenic cultivation and replica plating. She also is interested in defining differences between the two strains of C. elegans Bristol N2 and NIH that have been kept separate for a number of years. Jerzy Nowak from Poland worked 4 months on proteolytic enzymes in cleavage stages. He also started using the O'Farrell technique (J.B.C. 250, 4007-4021, 1975) for high resolution two-dimensional electrophoresis of proteins from eggs and worms. Randy Cassada will join the group as a staff member starting December 1975.

Kao, Michelle et al. (2015) International Worm Meeting "The Elegant Mind Club: Undergraduate Research Club to Explore the Minds of C. elegans."

[

International Worm Meeting,

2015]

For students, the study of model organisms presents an opportunity to learn various general science and research disciplines; however, each model organism has it's own set of advantages. Chiefly, Caenorhabditis elegans are excellent model organisms to study neuroscience and biophysics due to its availability, tractability, relatively simple nervous system, and it's patently observable behavior. Here in the Elegant Mind Club at UCLA, we provide undergraduate students a unique hands-on experience working with C. elegans and a chance to present their own scientific methods of interest, allowing them to explore the nature of scientific research and understand the conclusions drawn from their data. In our laboratory, undergraduate students are entirely responsible for maintaining and culturing the worms as well as building and refining their experimental systems. Direct involvement with the biological samples teaches students the discipline of working with chemicals and maintaining sterility. Published papers and online resources such as WormBook, WormAtlas, and Caenorhabditis Genetics Center provide students with a source of well-established methods and techniques to serve as a basis for their own studies. Manual practice in hardware development permits students to personally hone their experiment to be the most controlled and reproducible systems. As of now, systems for thermotaxis, electrotaxis, chemotaxis, phototaxis, durotaxis, as well as behavior within a magnetic field and the absence of stimuli have been reproduced and improved by our members. Our laboratory begun with a few core members and have expanded to accommodate more than 80 students from different universities over the world and we hope to encourage more students to approach scientific research with enthusiasm.

Nathaniel R Dudley et al. (2003) International Worm Meeting "Unwinding the mysteries of RNA interference: A conserved RNA helicase with double-stranded RNA binding domains is required for RNAi in C. elegans"

Nathaniel R Dudley, Bob Goldstein

[

International Worm Meeting,

2003]

Unwinding The Mysteries Of RNA Interference: A Conserved RNA Helicase With Double-Stranded RNA Binding Domains Is Required For RNAi In C. elegans Nathaniel R. Dudley and Bob Goldstein Department of Biology, University of North Carolina, Chapel Hill, NC 27599-3280 RNA-mediated interference (RNAi) is a phenomenon in which dsRNA triggers the sequence specific degradation of cognate mRNAs. Since its discovery (1), RNAi has quickly become a widely used tool to study gene function in a number of model organisms. However, its exact molecular nature remains incompletely defined. We have previously reported the development of a method that uses RNAi itself to identify new components of the RNAi machinery (RNAi to RNAi) (2). We have used this method to test candidate genes for roles in RNAi. Candidates were selected based on sequence, including predicted RNAses, helicases, DNA/RNA binding proteins, exosome components, RdRPs and chromatin modifiers. Of our initial 57 candidates, we have found 25 possible RNAi components, which we are testing further by determining whether loss of function mutants are also RNAi-deficient. We report here the identification of one such gene, rha-1 (T07D4.3), a new gene required for RNAi. We have found that rha-1 is required for RNAi to function in the germline: a recessive deletion mutant (tm329), predicted by sequence to be a null allele, fails in RNAi of germline-expressed genes. Additionally, we have found that germline expressed transgenes are desilenced in the rha-1 mutant. C. elegans RHA-1 contains a conserved helicase domain and two dsRNA binding domains and is homologous to Drosophila maleless and to RNA Helicase A in humans. Interestingly, these proteins have functions in the nucleus. We have created a rescuing GFP construct, which shows both nuclear and cytoplasmic localization. rha-1(tm329) has developmental phenotypes as well, including a temperature-sensitive Him phenotype and also has a significantly lower brood-size. Additionally, males mate poorly at 25C and have pronounced gonadal defects. Injection of siRNAs could not bypass the requirement for RHA-1, suggesting that RHA-1 acts downstream of siRNA production. We will present evidence that RHA-1 is required for RNAi and report further characterizations on the role of RHA-1 in the RNAi pathway. 1. Fire A, et al. (1998) Nature. 391(6669):806-11. 2. Dudley NR, Labbe JC, Goldstein B Proc Natl Acad Sci U S A. (2002) 99(7):4191-6.