Matsuda, S. et al. (2015) International Worm Meeting "Detection and measurement of abnormal posture under stressful conditions in age-related mutants ."

Nitta, N., Matsuda, S., Yanase, S., Sakashita, T., Tamura, M., Suzuki, M., Ishii, N.

[International Worm Meeting, 2015]

Oxidative stress threatens cellular constructs including lipids, proteins and nucleic acids, and causes or influence apoptosis, degenerative diseases and aging [1]. Recently, we found an abnormal posture in short-lived mutants such as mev-1 and daf-16, exposed to hyperoxia. In this study, we detected the hyperoxia-induced abnormal posture in mev-1 and daf-16 mutants compared with wild-type N2 and long-lived age-1 strains. 4-day-old animals placed on a NGM agar plate with a fixed bacterial lawn were exposed to hyperoxia (90% of oxygen) overnight. After the exposure, some animals were immediately recorded as digital images with up to 400-fold magnification of the microscope. The images were analyzed using image-processing software, Wriggle Tracker and Move-tr/2D (Library Co., Ltd., Tokyo), by a previously published method [2]. The body line was skeletonized and evenly divided into 12 segments. Subsequently, X- and Y-coordinates of each point on the body were acquired. To evaluate the posture of the body, we introduced a novel standard, namely the 'posture index' as the absolute average of the relative angle between adjacent dividing points. As a result of this investigation, we can not only intuitively but also quantitatively and statistically understand a tendency of the behavior in various postural responses under stressful conditions in the short-lived mutants.References 1. Yanase S. and Ishii N. (2008) Hyperoxia exposure induced hormesis decreases mitochondrial superoxide radical levels via Ins/IGF-1 signaling pathway in a long-lived age-1 mutant of Caenorhabditis elegans, J. Radiat. Res. 49: 211-218.2. Hattori Y. et al. (2012) Theoretical and evolutionary parameter tuning of neural oscillators with a double-chain structure for generating rhythmic signals, Neural Compt. 24: 635-675.

Matsuda, S. et al. (2009) International Worm Meeting "mboa-6 is required for incorporation of polyunsaturated fatty acids into phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine in C. elegans."

Gengyo-Ando, K., Inoue, T., Matsuda, S., Arai, H., Mitani, S.

[International Worm Meeting, 2009]

Glycerophospholipids, one of the major components of biological membranes, are comprised of various molecular species with different fatty acyl moieties. Previous studies have established that fatty acids of cellular phospholipids are distributed asymmetrically. In general, saturated fatty acids are esterified at the sn-1 position while polyunsaturated fatty acids (PUFAs) are esterified at the sn-2 position. It is widely believed that PUFAs are incorporated into glycerophospholipids after their de novo synthesis by remodeling of fatty acyl chains of newly synthesized phospholipid species. Over 40 years ago, it has been proposed that phospholipids of biological membranes are metabolically active and participate in a series of deacylation-reacylation reactions, which may lead to accumulation of PUFAs at the sn-2 position of the glycerol backbone; however, the genes that encode these enzyme have not yet been identified. Very recently, using C. elegans RNAi screen, we have identified lysophosphatidylinositol (LPI) acyltransferase (mboa-7/LPIAT) which specifically transfers PUFAs into the sn-2 position of LPI. mboa-7/LPIAT belongs to the membrane-bound O-acyltransferase (MBOAT) family and is widely conserved in variety of species ranging from C. elegans to mammals. In this study, we found mboa-6, another member of the MBOAT family in C. elegans, as an acyltransferase which catalyzes incorporation of PUFAs into lysophosphatidylcholine (LPC), lysophosphatidylserine (LPS) and lysophosphatidylethanolamine (LPE). Knockdown of mboa-6 reduced incorporation of exogenous PUFAs into phosphatidylcholine (PC), phosphatidylserine (PS) and phosphatidylethanolamine (PE) in C. elegans. In in vitro assays, overexpression of mboa-6 and its human orthologue resulted in great increases in LPC, LPS and LPE acyltransferase activities but not in LPI or lysophosphatidic acid (LPA) acyltransferase activities. These results indicate that mboa-6 is a lysophospholipid acyltransferase (LPLAT) acting preferentially on LPC, LPS and LPE. Knockdown of mboa-6 caused several developmental defects such as early larval arrest, slow growth, and a dumpy morphology during postembryonic development, suggesting that PUFA-containing PC, PS, or PE are required for normal larval growth and morphology. Identification of mboa-7/LPIAT and mboa-6/LPLAT will reveal the physiological significance of fatty acid molecular species in membrane phospholipids.

Pedersen J S et al. (2010) Aging, Metabolism, Stress, Pathogenesis, and Small RNAs, Madison, WI "A study of ADan and ABri aggregation and toxicity in C. elegans models"

Morimoto R I, Wang N, Pedersen J S, Eichel K

[Aging, Metabolism, Stress, Pathogenesis, and Small RNAs, Madison, WI, 2010]

Familial Danish- and Familial British Dementias (FDD/FBD) are rare dominant neurodegenerative disorders mapped to mutations near the stop codon of the BRI2 gene that result in the synthesis of C-terminally elongated amyloidogenic peptides ADan and ABri, instead of the wild-type Bri23 peptide1. Neuropathological lesions in these disorders are similar to Alzheimer's disease (AD), including neurofibrillary tangles, parenchymal amyloid and pre-amyloid deposits. These similarities lead to the hypothesis that protein aggregation in general, irrespective of sequence can result in similar neuropathology. However, FDD and FBD are also manifested by early onset of cataract, deafness and/or ataxia not seen in AD. To further investigate the aggregation and toxicity of ADan and ABri, we have generated transgenic C. elegans animals expressing these peptides as YFP fusions. Animals expressing ADan and ABri exhibit massive, very early onset formation of brightly fluorescent YFP aggregates, while the Bri23 peptide fusions remain soluble. Despite this, behavioral studies indicate that the effects of ADan and ABri aggregation in neurons and muscle cells are significantly different from what is seen for Abeta1-42 and polyglutamine. Crosses between the different models reveal that Abeta1-42 forms heterogeneous co-aggregates with ADan, but not with Q40, bringing the attached CFP/YFP pair into FRET distances similar to that observed for homogeneous aggregates. Recent results indicate that wild-type BRI2-protein may inhibit production and aggregation of the Abeta peptide2, suggesting that loss of function as well as disruptive effects of ADan/ABri aggregation could directly or indirectly affect aggregation of Abeta, leading to the observed early onset of AD-like pathology. 1. A. Rostagno, Y. Tomidokoro, T. Lashley et al., Cell Mol Life Sci 62 (16), 1814 (2005); S. H. Kim, J. W. Creemers, S. Chu et al., The Journal of biological chemistry 277 (3), 1872 (2002). 2. S. Matsuda, L. Giliberto, Y. Matsuda et al., J Neurosci 28 (35), 8668 (2008); J. Kim, V. M. Miller, Y. Levites et al., J Neurosci 28 (23), 6030 (2008).

Pinan-Lucarre, Berangere et al. (2009) International Worm Meeting "Transgenic C. elegans expressing the fungal prion protein HET-s as a model for the study of amyloid infectivity and toxicity."

Qiao, Yujie, Saupe, Sven, Pinan-Lucarre, Berangere, Driscoll, Monica

[International Worm Meeting, 2009]

Amyloids are protein fibers rich in beta sheet structure that are associated with numerous neurodegenerative diseases, including Alzheimer disease. Amyloids have two major biological properties. First, they can be infectious--meaning that they can spread the altered amyloid conformation to the same protein or even among proteins of distinct primary sequence (the latter phenomenon is known as cross-polymerization). Infectious amyloids are called prions. Second, amyloids can be toxic, whether they are infectious or not. Both properties have tremendous fundamental and biomedical impact. [Het-s] is a prion of the fungus Podospora anserina involved in a defense cell suicide mechanism named heterokaryon incompatibility, which disables mixing of different strains by somatic fusion, an ubiquitous feature in filamentous fungi. The HET-s protein exists in a soluble state or in an aggregated, prion state named [Het-s]. The [Het-s] prion is not toxic by itself in P. anserina or in yeast. However cell death is rapidly triggered by the lethal interaction between 2 alleles of the het-s locus: het-S (het-"large S") and het-s (het-"small s") when the HET-s protein adopts the prion form. This prion has been extensively characterized and it is the only prion for which tridimensional structure is available to date. The critical point is that in the [Het-s] system, transgenic expression of het-s allows propagating a non-toxic prion while co-expression of het-s and het-S generates toxicity. We constructed strains expressing het-s or the prion domain only het-s(218-289) (the 218-289 fragment of the protein) as GFP fusions under the control of the ubiquitous promoter dpy-30. The fluorescence of pdpy-30het-s::gfp appears to be mainly diffuse in the cytoplasm while it shows strong aggregation in pdpy-30het-s(218-289)::gfp strains. We constructed strains expressing het-s(218-289)::gfp in the touch neurons using mec-4 promoter and observed that the fusion protein is mostly aggregated into a large fiber spanning the cell body and the proximal part of the axon. These aggregates remain to be shown as infectious [Het-s] amyloids. In future experiments, we will aim to generate neuronal toxicity through the co-expression of het-s(218-289) and het-S. This C. elegans model for prion studies, filling a gap between simple fungal systems and highly complex mammalian systems, might allow a better understanding of the molecular and cellular basis of amyloid infectivity and toxicity.

Dana L. Miller et al. (2007) International Worm Meeting "Adaptation to hydrogen sulfide increases thermotolerance and lifespan."

Mark B. Roth, Dana L. Miller

[International Worm Meeting, 2007]

Hydrogen sulfide (H₂S), which is naturally produced in animal cells, has been shown to effect physiological changes that improve the capacity of mammals to survive environmental changes. We have investigated the physiological response of C. elegans to H₂S to begin to elucidate the molecular mechanisms of H₂S action. We show that nematodes exposed to H₂S are apparently healthy and do not exhibit phenotypes consistent with metabolic inhibition. However, we observed that animals exposed to H₂S had increased thermotolerance and lifespan and survived subsequent exposure to otherwise lethal concentrations of H₂S. Increased thermotolerance and lifespan is not observed in the sir-2.1(ok434) deletion mutant exposed to H₂S. However, mutants in the insulin signaling pathway (both daf-2 and daf-16), animals with mitochondrial dysfunction (isp-1 and clk-1) and a genetic model of caloric restriction (eat-2) all exhibit H₂S-induced increased thermotolerance. These data suggest that H₂S activates a pathway including SIR-2.1 that is separate from dietary restriction and insulin signaling that results in increased lifespan. Moreover, these studies suggest that SIR-2.1 activity may translate environmental change into physiological alterations that improve survival. It is interesting to consider the possibility that the mechanisms by which H₂S increases thermotolerance and lifespan in nematodes are conserved, and that studies using C. elegans may help explain beneficial effects observed in mammals exposed to H₂S.

Oppenheimer AJ et al. (1995) International C. elegans Meeting "G-PROTEIN SIGNALING IN C. ELEGANS"

Kaplan JM, Oppenheimer AJ

[International C. elegans Meeting, 1995]

We are interested in determining how G-proteins modulate the activity of ion channels in the C. elegans nervous system. To generate behavioral phenotypes dependent on G-protein signaling, we targeted expression of mammalian G-proteins to a set of neurons including those controlling foraging and locomotion (RMD, AVA, AVB, AVD, and PVC). Ectopic expression of several G-proteins under the control of the not-3 promoter results in behavioral defects. Expression of constitutively active rat G-alpha-i1 causes uncoordinated forward and backward locomotion. Expression of wild-type rat G-alpha-s impairs backward locomotion, while constitutively active rat G-alpha-s causes defective backward locomotion and lethargy. In order to identify components of the G-alpha-s signaling pathway, we have initiated a screen for mutations that suppress the behavioral effects of activated G-alpha-s expression. In a screen of approximately 7000 haploid genomes, we have identified 11 independent mutations that improve backward locomotion. Several of these mutations result in a hyperactive phenotype in the absence of activated G-alpha-s expression. Mapping and complementation tests will determine whether the suppressors are allelic to other hyperactive mutants isolated by D. Elkes and J. Kaplan. By cloning the suppressor genes, we hope to identify ion channels regulated by G-alpha-s as well as other components of the G-alpha-s signaling pathway. We also plan to determine whether the behavioral effects of G-alpha-s expression are mediated by known second messenger systems. We are first testing the role of cAMP-dependent protein kinase (PKA), because it is activated by G-alpha-s in other systems and has been shown to phosphorylate ion channels. If G-alpha-s signals through PKA, increasing the level of PKA activity should mimic the effect of activated G-alpha-s expression, and inhibiting PKA should suppress the effect of activated G- alpha-s expression.

Oppenheimer AJ et al. (1996) East Coast Worm Meeting "G-alpha-s signaling in C. elegans"

Kaplan JM, Oppenheimer AJ

[East Coast Worm Meeting, 1996]

We are interested in determining how G-proteins modulate the activity of ion channels in the C. elegans nervous system. To generate phenotypes dependent on G-protein signaling, we expressed mammalian G-proteins under the control of the glr-1 promoter, which drives expression in a set of neurons including those controlling foraging and locomotion (RMD, AVA, AVB, AVD, and PVC). Expression of wild-type rat G-alpha-s impairs backward locomotion, while consitutively active rat G-alpha-s causes paralysis and neuronal degeneration in a subset of G-alpha-s-expressing cells, primarily the PVC and AVD neurons. Our model for the neurodegeneration is that activated G-alpha-s is misregulating an ion channel in AVD and PVC, causing osmotic imbalance and consequent swelling. Degenerating neurons appear enlarged and vacuolated, similar to those in worms carrying gain-of-function mutations in deg-1 and other degenerins. We tested whether activated G-alpha-s is killing cells by misregulating known ion channels. Loss-of-function mutations in deg-1, mec-6, unc-36, unc-2, and egl-19 were unable to suppress the degenerations, suggesting that G-alpha-s is not acting through these degenerins or calcium channels. Mutations in ced-3 and ced-4 do not prevent G-alpha-s-induced degenerations, indicating that G-alpha-s is not killing through apoptosis. To identify the components of the signaling pathway downstream of G-alpha-s, we screened for mutations that suppress the paralysis caused by activated G-alpha-s. In a screen of 7,760 haploid genomes, we identified 8 mutations that suppress paralysis and neurodegeneration and 8 mutations that suppress only paralysis. Five of the degeneration suppressors map to chromosome III and may be allelic. These mutations are semidominant, and several cause a hyperactive phenotype in the absence of activated G-alpha-s. Because hyperactivity also results from mutations in the Go signaling pathway, our suppressor mutations may identify a gene invovled in both the Gs and Go signaling pathways. We are continuing to map and characterize the suppressor genes.

Costi D. Sifri et al. (2007) International Worm Meeting "Immune evasion by staphylococcal exopolysaccharide during C. elegans infection."

Jakob Begun, Dietrich Mack, Costi D. Sifri, Jessica M. Gaiani, Frederick M. Ausubel, Holger Rohde, Stephen B. Calderwood

[International Worm Meeting, 2007]

We have previously shown that C. elegans can serve as a model host for the study of S. aureus pathogenesis and host innate immunity. Here we characterize nematode killing by S. epidermidis. C. elegans fed S. epidermidis survive 3-5 days, which is ~1 day longer than their lifespan on S. aureus. Inactivation of the ica operon (the biofilm exopolysaccharide [PIA] biosynthetic operon and Yersinia hms homolog) in S. epidermidis strain M10 greatly reduces nematode killing compared to the isogenic parental strain 9142 and a plasmid complemented mutant. In S. aureus, overproduction of PIA due to derepression of ica augments nematocidal activity. FITC-WGA labeling confirmed the presence of PIA within the digestive tracts of worms fed 9142 but not M10. Attachment of bacteria or bacterial matrix to the nematode cuticle was not observed. Nematodes briefly exposed to 9142 could not be rescued by transfer to M10, and worms fed dilutions of 9142 in M10 at ratios as low as 1:10e4, respectively, still die. Pulse/chase experiments using 9142, M10, and a second PIA-deficient strain, ATCC 12228, showed that durable colonization requires an intact ica operon. Disruption of N2 worms fed 1:100 9142/M10 mixtures for 20h confirmed significant enrichment of 9142 within the digestive tract of N2 but not sek-1 animals. Moreover, sek-1 and pmk-1 animals were found to be equally sensitive to M10- and 9142-mediated killing, in contrast to wild-type N2 worms. Several conclusions can be drawn from these studies. (a) S. epidermidis can infect and kill worms. (b) Disruption of PIA biosynthesis abrogates S. epidermidis virulence, while overproduction of PIA in S. aureus augments virulence. (c) PIA-producing S. epidermidis has a competitive advantage over PIA-deficient S. epidermidis within the nematode digestive tract. (d) Genetic analysis suggests that PIA may protect luminal staphylococci from SEK-1 p38 MAPK-regulated immune responses.

Scholer, Lone V. et al. (2011) International Worm Meeting "Characterization of pathogenesis of S. aureus and involvement of checkpoint response in C. elegans."

Hansen, Frederik D, Scholer, Lone V., Fuursted, Kurt, Noerregaard, Steffen, Olsen, Anders

[International Worm Meeting, 2011]

Staphylococcus aureus (S. aureus) is a frequent colonizer of humans contributing to normal bacterial flora in nose, skin and mucosa. In addition, it is an important human pathogen causing various diseases ranging from superficial skin infections to life-threatening infections. Methicillin resistant S. aureus (MRSA) strains are rapidly spreading in the community (CA-MRSA) and is consequently becoming a more serious health threat. The capacity of S. aureus to cause this spectrum of human diseases reflects an ability to adapt to distinct microenvironments in the human body and suggests that the pathogenesis of S. aureus infection is a complex process involving a diverse array of virulence determinants that are coordinately expressed at different stages of infection. Studies have shown that virulence factors are very differently distributed among strains and are not always regulated in the same way, reflecting the ability of S. aureus to adapt and survive in different environmental niches and resist antibiotic treatment. C. elegans has previously been established as a model host for the study of S. aureus pathogenesis and CA-MRSA strains are capable of killing C. elegans[1]. We have screened thirty clinical MRSA isolates and found that in terms of pathogenicity they can be divided into separate classes. Using these classes we wish to identify new virulence markers and novel immunity pathways activated in response to S. aureus infection. We are particularly interested in evaluate virulence determinants during colonization versus infection and establishing their clinical relevance in humans. Inactivation of some S-M checkpoint proteins increase stress resistance and lifespan of C. elegans[2]. Since there may exist a potential correlation between stress response and immune function we are studying the role of checkpoint proteins to S. aureus exposure. We have identified several novel genes with potential checkpoint function which increase lifespan and thermotolerance. We find that that mutation of one of these, the transmembrane protein NDG-4, also confers resistance towards S. aureus infection. Epistasis analysis reveals that increased tolerance towards S. aureus infection is partially dependent on insulin signaling but that another unidentified component is involved as well. 1. Sifri, C.D., et al. 2003. 71(4): p. 2208-17. 2. Olsen, A., M.C. Vantipalli, and G.J. Lithgow. Science, 2006. 312(5778): p. 1381-5.

Jafa Armagost et al. (2007) International Worm Meeting "Investigating Potential Bacterial Sources of Dopaminergic Neurotoxicity in C. elegans."

Kim A. Caldwell, Guy A. Caldwell, Tyler Hodges, Jeana Blalock, Julie B. Olson, Jafa Armagost

[International Worm Meeting, 2007]

Parkinsons disease (PD) involves the progressive loss of dopamine (DA) neurons from the substantia nigra pars compacta (SNpc). Genetic forms of PD account for only 5-10% of known cases and environmental factors appear pivotal to sporadic causality. Evidence from both environmental and genetic studies of PD indicates that overloading the ubiquitin-proteasome system is a causative risk factor for PD. Furthermore, when proteasome inhibitors were injected directly into the brains of rats, PD-like symptoms resulted (McNaught et al., 2004; Ann Neurol. 56:149-162). Many proteasome inhibitors are isolated from bacterial strains within the order Actinomycetales, which are well known for the production of secondary metabolites. To determine if exposure to Actinomycetes could cause DA neurodegeneration in C. elegans, we exposed worms to three different species of Streptomyces (S. venezuelae, S. griseus, and S. coelicolor). When worms were grown on either S. griseus or S. coelicolor no DA neurodegeneration was observed. However worms exposed to S. venezuelae displayed DA neurodegeneration that increased over time. The causual factor is excreted by S. venezuelae, as conditioned media is sufficient for the neurodegenerative effect. This factor does not enter DA neurons through the DA transporter because degeneration is observed in dat-1 mutant animals. Further characterization of the S. venezuelae activity shows that DA degeneration does not result from generalized stress response or the unfolded protein response but may occur as a result of proteasome inhibition. Initial efforts to purify the factor have revealed that it is lipophilic and does not fit the molecular profile of known proteasome inhibitors.