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Neuron,
2001]
Weighing in at about 5 ug, with 302 neurons and 5000 synapses, C. elegans is unlikely to prove theorems, write poetry, or challenge Mike Tyson. Still, remarkable behavioral complexity is packed into this tiny worm.
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Biochem J,
2016]
Aging is often accompanied by a decline in mitochondrial mass and function in different tissues. Additionally, cell resistance to stress is frequently found to be prevented by higher mitochondrial respiratory capacity. These correlations strongly suggest mitochondria are key players in aging and senescence, acting by regulating energy homeostasis, redox balance and signalling pathways central in these processes. However, mitochondria display a wide array of functions and signalling properties, and the roles of these different characteristics are still widely unexplored. Furthermore, differences in mitochondrial properties and responses between tissues and cell types, and how these affect whole body metabolism are also still poorly understood. This review uncovers aspects of mitochondrial biology that have an impact upon aging in model organisms and selected mammalian cells and tissues.
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Curr Opin Neurobiol,
1999]
Analysis of axon guidance mechanisms in vertebrates, Caenorhabditis elegans, and Drosophila melanogaster has led to the identification of several signaling pathways, many of which are strikingly conserved in function. Recent studies indicate that several axon guidance mechanisms are highly conserved in all animals, whereas others, though still conserved in a general sense, show strong evolutionary divergence at a detailed mechanistic level.
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Development,
1999]
One hundred years after Weismann's seminal observations, the mechanisms that distinguish the germline from the soma still remain poorly understood. This review describes recent studies in Caenorhabditis elegans, which suggest that germ cells utilize unique mechanisms to regulate gene expression. In particular, mechanisms that repress the production of mRNAs appear to be essential to maintain germ cell fate and viability.
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Hosseinian S, Gidziela A, Mech AM, Sealy IM, Brennan CH, Torres-Perez JV, Leggieri A, Petrazzini MEM, Malanchini M, Sheardown E, Busch-Nentwich EM
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Neurosci Biobehav Rev,
2022]
Psychiatric disorders represent a significant burden in our societies. Despite the convincing evidence pointing at gene and gene-environment interaction contributions, the role of genetics in the aetiology of psychiatric disease is still poorly understood. Forward genetic screens in animal models have helped elucidate causal links. Here we discuss the application of mutagenesis-based forward genetic approaches in common animal model species: two invertebrates, nematodes (Caenorhabditis elegans) and fruit flies (Drosophila sp.); and two vertebrates, zebrafish (Danio rerio) and mice (Mus musculus), in relation to psychiatric disease. We also discuss the use of large scale genomic studies in human populations. Despite the advances using data from human populations, animal models coupled with next-generation sequencing strategies are still needed. Although with its own limitations, zebrafish possess characteristics that make them especially well-suited to forward genetic studies exploring the aetiology of psychiatric disorders.
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ACS Chem Biol,
2006]
Whereas the C. elegans genome was sequenced many years ago, the role of small molecule signals in its biology is still poorly understood. A recent publication reports the identification of two steroidal signaling molecules that regulate C. elegans reproductive development and dauer diapause via the nuclear receptor DAF-12. The two compounds, named dafachronic acids, represent the first endogenous ligands identified for any of the 284 nuclear receptors in C. elegans .
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Adv Exp Med Biol,
2014]
MicroRNAs (miRNAs) are small noncoding RNAs that direct posttranscriptional regulation of specific target genes. Since their discovery in Caenorhabditis elegans, they have been associated with the control of virtually all biological processes and are known to play major roles in development and cellular homeostasis. Yet the biological roles of most miRNAs remain to be fully known. Furthermore, the precise rules by which miRNAs recognize their targets and mediate gene silencing are still unclear. Systematic identification of miRNAs and of the RNAs they regulate is essential to close these knowledge gaps. Studies in C. elegans have been instrumental not only in the discovery phase of miRNA biology but also in the elucidation of mechanisms regulating miRNA expression, target recognition and regulation. This chapter highlights some of the main challenges still present in the field, while introducing the major studies and methods used to find miRNAs and their targets in the worm.
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Crit Rev Biochem Mol Biol,
1999]
Although largely hidden from public view, nematodes are among the most numerous and ecologically diverse multicellular organisms inhabiting the planet. Although most species are microbivorous, numerous species are economically or medically important parasites of plants and animals. As Cobb wrote 80 years ago, "If all the matter in the universe except the nematodes were swept away, our world would still be dimly recognizable, and if, as disembodied spirits, we could then investigate it, we should find its mountains, hills, vales, rivers, lakes and oceans represented by a film of nematodes. The locations of towns would be decipherable, since for every massing of human beings there would be a corresponding massing of certain nematodes. The location of the various plants and animals would still be decipherable, and has we sufficient knowledge, in many cases their species could be determined by an examination of their erstwhile nematode parasites".
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Biochim Biophys Acta,
1998]
Intracellular membrane transport is mediated predominantly by vesicles which bud from one compartment and fuse specifically with the next compartment in the pathway, resulting in delivery of cargo. COPI-coated vesicles were first identified as intermediates in intra-Golgi transport and subsequent work has shown that they are also involved in transport between the endoplasmic reticulum and the Golgi complex. The COPI coat components have been characterised in detail at the molecular level and a role for membrane proteins and lipids in membrane recruitment of COPI has been uncovered. However, precisely how these distinct membrane components regulate coat recruitment is still unclear and is currently a matter for debate. Furthermore, it is still not clear at exactly how many transport steps COPI is involved and whether it mediates secretory transport in the anterograde or retrograde direction or both. This review focuses on our understanding of COPI structure and function and describes recent findings on the sites of action of COPI in animal cells.
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Methods Cell Biol,
2011]
Although the general events surrounding fertilization in many species are well described, the molecular underpinnings of fertilization are still poorly understood. Caenorhabditis elegans has emerged as a powerful model system for addressing the molecular and cell biological mechanism of fertilization. A primary advantage is the ability to isolate and propagate mutants that effect gametes and no other cells. This chapter provides conceptual guidelines for the identification, maintenance, and experimental approaches for the study fertility mutants.