Plenefisch JD et al. (1994) Worm Breeder's Gazette "Cloning mua-3: Some Observations on the New Molecular Era"

Plenefisch JD, Hedgecock EM

[Worm Breeder's Gazette, 1994]

Cloning mua-3: some observations on the new Molecular Era John Plenefisch and Edward Hedgecock, Dept. of Biology, Johns Hopkins University, Baltimore MD 21218

Gunaratna, Miluka et al. (2011) International Worm Meeting "Construction of Arrayed Toxicology Reporter Panels using Transcript-activated Fluorescent Reporters in Transgenic Nematodes."

Manfredi, John, Hopkins, Christopher, Gunaratna, Miluka

[International Worm Meeting, 2011]

Toxicology assessment of chemicals is used for government regulatory decisions, industrial and environmental safety, and design of new therapeutic compounds. We are developing a panel of nematodes for use in high-throughput toxicology assays. Promoters of toxin-responsive genes are fused to fluorescent-protein coding sequence and inserted C. elegans genome by the patented MosSCI transgenesis procedure. The resulting panel of 96 toxicology-reporter nematodes are exposed to toxin and scored for gene activation by fluorescent plate-reader assays. Detection of a gene-activation is observed as increased fluorescence in a reporter strain. The genes chosen for reporter development are involved in heat shock, oxidative stress, heavy metals, and xenobiotic response. Over 300 genes are under consideration for development as toxin-responsive genes. Currently, 64 gene-activation reporters have been designed and 21 have progressed to transgenic strain candidates (hsp-1, hsp-3, hsp-4, hsp-6, hsp-16.2, hsp-16.41, hsp-17, hsp-60, dnj-13, daf-21, gst-4, gst-38, ugt-1, ugt-13, gcs-1, skn-1, cdr-1, mtl-2, cyp-13A7, cyp-14A3, cyp-35A2). To control for toxin-induced population differences, the reporter strains are made in the background of the constitutively-expressed unc-47::GFP reporter gene. To enable high-throughput liquid handling of strains, we have attempted to observe expression in liquid media using the hsp-16.41::hRFP reporter strain. Heat-shock activation of the reporter shows greatly reduced expression in liquid media relative to growth on NGM plates. We have developed an artificial-soil substitute to solve the low expression problem of liquid media. Expression of heat-shock reporters in the artificial soil gives expression comparable to NGM plates. Ease of application of artificial-soil to the 96-well format enables the design of a high-throughput platform for rapid gene-activation detection.

Parham C et al. (1994) Worm Breeder's Gazette "Tc4 and Tc5: What Makes Them Move and Why It Matters"

Beinhorn J, Collins JJ, Butze K, Parham C

[Worm Breeder's Gazette, 1994]

Tc4 and Tc5: what makes them move and why it matters Christi Parham, Kristie Butze, Joanna Beinhorn and John Collins. Dept. of Biochemistry and Molecular Biology, University of New Hampshire. Durham, NH 03824

Almeida CA et al. (1994) Worm Breeder's Gazette "Function of a Domain of the Myosin Heavy Chain Implicated in Familial Hypertrophic Cardiomyopathy"

Collins JJ, Almeida CA, Swift KE

[Worm Breeder's Gazette, 1994]

Function of a Domain of the Myosin Heavy Chain Implicated in Familial Hypertrophic Cardiomyopathy Craig A. Almeida, Kerry E. Swift and John J. Collins Department of Biochemistry and Molecular, University of New Hampshire, Durham, NH 03820

Chalfie M (1993) J Neurobiol "Touch receptor development and function in Caenorhabditis elegans."

Chalfie M

[J Neurobiol, 1993]

Mutations causing a touch-insensitive phenotype in the nematode Caenorhabditis elegans have been the basis of studies on the specification of neuronal cell fate, inherited neurodegeneration, and the molecular nature of mechanosensory transduction. (C) 1993 John Wiley & sons, Inc.

Maine EM et al. (1994) Worm Breeder's Gazette "Mutations That Enhance glp-1 Identify Genes Required For Various Aspects of Germline Development."

Gelber MB, Smardon AM, Shu P, Qiao L, Maine EM, Lissemore JL

[Worm Breeder's Gazette, 1994]

Mutations that enhance glp-1 identify genes required for various aspects of germline development. Eleanor Maine, Li Qiao, Jim Lissemore-, Pei Shu, Anne Smardon, and Melanie Gelber. Biology Dept., Syracuse University, Syracuse, NY 13244 and Biology Dept., John Carroll University, Cleveland, OH 44118.

Chalfie M (1998) Nature "Genome sequencing. The worm revealed."

Chalfie M

[Nature, 1998]

In 1983, John Sulston and Alan Coulson began to construct a complete physical map of the genome of the nematode worm Caenorhabditis elegans, and started what became known as the C. elegans Genome Project. At the time, several people wondered why John, who had just described all of the cell divisions in C. elegans (the cell lineage), was interested in this project rather than in a more 'biological' problem. He replied by joking that he had a "weakness for grandiose, meaningless projects". In 1989, as the physical map approached completion, the Genome Project, now including Bob Waterston and his group, embarked on the even more ambitious goal of obtaining the complete genomic sequence

Hotez PJ et al. (1993) Parasitol Today "Hookworm larval infectivity, arrest and amphiparatenesis: the Caenorhabditis elegans Daf-c paradigm."

Hotez PJ, Schad GA, Hawdon JM

[Parasitol Today, 1993]

Arrested development dramatically alters the life history of some species of soil-transmitted nematodes and elicits profound variations in the epidemiology of the infections they cause. Here, Peter Hotez, John Hawdon and Gerhard Schad show how an understanding of the cellular and molecular bases of arrested development may lead to new approaches for the control of ancylostomiasis and related infections.

Waterston RH et al. J Neurogenet "John Sulston (1942-2018): a personal perspective."

Waterston RH, Moerman DG

[J Neurogenet]

John Sulston changed the way we do science, not once, but three times - initially with the complete cell lineage of the nematode <i>Caenorhabditis elegans</i>, next with completion of the genome sequences of the worm and human genomes and finally with his strong and active advocacy for open data sharing. His contributions were widely recognized and in 2002 he received the Nobel Prize in Physiology and Medicine.

Chalfie M J Neurogenet "A touching story."

Chalfie M

[J Neurogenet]

A slide taped to a window at the Woods Hole Marine Biology Laboratory was my first introduction to the touch receptor neurons of the nematode <i>Caenorhabditis elegans</i>. Studying these cells as a postdoc with Sydney Brenner gave me a chance to work with John Sulston on a fascinating set of neurons. I would never have guessed then that 43 years later I would still be excited about learning their secrets.