Domeier ME et al. (1997) International C. elegans Meeting "EXPRESSION OF pha-1 DURING EMBRYOGENESIS"

Domeier ME, Mango SE

[International C. elegans Meeting, 1997]

At about the 200 cell stage of embryogenesis, 27 precursors are born that will ultimately produce the 80 cells of the pharynx. Initial studies using a pha-1::lacZ construct determined that pha-1 was expressed at the 200 cell stage, in pharynx and body wall muscle precursors1. To visualize the pharyngeal precursors in living embryos, we created two pha-1::GFP constructs. The first construct was a translational fusion that contained 3.7 kb of upstream sequences plus the entire pha-1 coding domain fused to GFP. The second was a transcriptional fusion carrying pha-1 5! sequences and a nuclear-localized GFP::lacZ cassette. GFP expression was assayed from stable lines in N2 embryos. Surprisingly, both GFP constructs were expressed in multiple cell types in addition to pharynx and body wall muscle precursors. For example, hypodermis, neurons and intestinal cells all expressed pha-1::GFP. Thus, pha-1 may be expressed more globally than first reported. Furthermore, the pha-1::GFP protein fusion was found predominantly in the cytoplasm. This construct was able to rescue pha-1 mutants, suggesting that endogenous PHA-1 may be a cytoplasmic protein as well. To determine the expression pattern of endogenous PHA-1, we are making anti-PHA-1 antibodies. 1.Granato M., Schnabel H. and Schnabel R. (1994) Development 120, 3005-3017. We thank Heinke and Ralf Schnabel for generously sending us alleles of pha-1 as well as pha-1 expression constructs.

Domeier ME et al. (2000) Science "A link between RNA interference and nonsense-mediated decay in Caenorhabditis elegans."

Morse DP, Knight SW, Portereiko MF, Mango SE, Bass BL, Domeier ME

[Science, 2000]

Double-stranded RNA (dsRNA) inhibits expression of homologous genes by a process involving messenger RNA degradation. To gain insight into the mechanism of degradation, we examined how RNA interference is affected by mutations in the smg genes, which are required for nonsense-mediated decay. For three of six smg genes tested, mutations resulted in animals that were initially silenced by dsRNA but then recovered; wild-type animals remained silenced. The levels of target messenger RNAs were restored during recovery, and RNA editing and degradation of the dsRNA were identical to those of the wild type. We suggest that persistence of RNA interference relies on a subset of smg genes.

Kuroyanagi H et al. (2014) Worm "Comprehensive analysis of mutually exclusive alternative splicing in C. elegans."

Takei S, Kuroyanagi H, Suzuki Y

[Worm, 2014]

Mutually exclusive selection of one exon in a cluster of exons is a rare form of alternative pre-mRNA splicing, yet suggests strict regulation. However, the repertoires of regulation mechanisms for the mutually exclusive (ME) splicing in vivo are still unknown. Here, we experimentally explore putative ME exons in C. elegans to demonstrate that 29 ME exon clusters in 27 genes are actually selected in a mutually exclusive manner. Twenty-two of the clusters consist of homologous ME exons. Five clusters have too short intervening introns to be excised between the ME exons. Fidelity of ME splicing relies at least in part on nonsense-mediated mRNA decay for 14 clusters. These results thus characterize all the repertoires of ME splicing in this organism.

Zuckerman BM et al. (1991) International C. elegans Meeting "A MICROBIAL EGG-LAYING INHIBITING FACTOR FOR CAENORHABDITIS ELEGANS."

Zuckerman BM, Willett JW, Dicklow MB

[International C. elegans Meeting, 1991]

A microbial exometabolite (ME) has been isolated which inhibits egg- laying in Caenorhabditis elegans. Nematodes exposed to active concentrations of the factor, and maintained at 22C in axenic culture ( liver extract medium), mature and grow normally. Eggs are fertilized and larvae develop and move within eggs as in untreated nematodes. However, eggs are not laid, though occasionally hatch occurs within the gonad. In shake culture of the microbe, active yellow-pigmented ME appears at 3-4 days. A concentration of 8 parts ME: 1 part liver extract completely inhibits egg-laying. Concentrations of 4 ME: 1 liver extract and 2 ME: 1 liver extract, inhibit egg-laying about 50 and 25%, respectively. Sections from nematodes exposed to extracts of a microbe showing similar properties examined by transmission electron microscopy indicated that the constrictor and dilator muscles associated with vulval function in egg-laying appear normal. ME is thermostable (at 100C for 5 min.), and the active fraction does not pass through 6,000-8,000 MW dialysis tubing. About one-half of the activity is lost when ME is dialyzed through 12,000 14,000 MW membrane tubing, suggesting the presence of at least two active fractions. Trypsinization obliterated activity. Thus far, the double control experiment with trypsin inhibitor has not yielded definitive results. SDS gel electrophoresis of boiled ME, with molecules below 8,000 MW removed by dialysis, revealed several strong protein bands, one or more of which may be ME. ME purification and characterization studies continue.

Sulston JE (2003) Biosci Rep "C. elegans: the cell lineage and beyond."

Sulston JE

[Biosci Rep, 2003]

Thank you so very much for inviting me to be here. It gives me a mingled sense of humility at how much I owe to others, and of joy that the collective work on the worm has been recognized in this way.

Sulston JE (2003) Chembiochem "Caenorhabditis elegans: the cell lineage and beyond (Nobel lecture)."

Sulston JE

[Chembiochem, 2003]

Thank you so very much for inviting me to be here. It gives me a mingled sense of humility at how much I owe to others, and of joy that the collective work on the worm has been recognised in this way.

MF Portereiko et al. (1999) International C. elegans Meeting "Pharyngeal Morphogenesis"

MF Portereiko, ME Domeier, SE Mango

[International C. elegans Meeting, 1999]

Our lab is interested in studying pharyngeal morphogenesis, the process whereby cells of the pharyngeal primordium elongate to form a tube that connects to the buccal cavity. We have taken two approaches towards understanding this process. First, we analyzed the movements associated with wildtype pharyngeal morphogenesis. We built a chimera composed of a digestive tract-specific promoter controlling GFP expression, and we targeted GFP to the plasma membrane by including the 'CAAX' sequence from mig-2 1 . We introduced the GFP chimera into wildtype animals and observed the behavior of pharyngeal cells using time-lapse microscopy 2 . Our preliminary studies suggest that pharyngeal morphogenesis depends in large part on directed cell shape changes, and not on intercalation (e.g. convergence/extension) nor cellular migration. The cumulative effects of anterior pharyngeal cells increasing their longitudinal dimension appears to be sufficient to bridge the distance between the pharyngeal primoridum and the nascent buccal cavity. These data suggest that directed changes in the actin cytoskeleton may be important for pharyngeal elongation. Second, we searched for loci involved in pharyngeal morphogenesis using a deficiency screen. We surveyed approximately a third of the genome for deficiencies with pharyngeal elongation defects and chose two for further analysis. i) mnDf90 embryos form a pharyngeal primordium that fails to attach to the buccal cavity (see abstract by Lange and Mango); ii) uDf1 embryos attach to the buccal cavity similar to wildtype embryos. However, these mutants form a bifurcated tube with two lumens 3 . This phenotype could reflect a defect in cellular positioning within the primordium. Alternatively, the cell shape or polarity changes that normally occur during morphogenesis may be disrupted. These embryos contain the wildtype number of pharyngeal cells, and appear to differentiate normally. Our current goals are to follow cellular behaviors in the deficiency embryos as well as identify single point mutations that mimic the deficiency phenotypes. 1. Zipkin et al., 1997 2. Special thanks to Bill Mohler, John White and the IMR for their help. 3. see also Terns et al., 1997

White JG (2000) Int J Dev Biol "Worm tales."

White JG

[Int J Dev Biol, 2000]

1969 was a landmark year. But for me it was not Neil Armstrong's giant leap or Woodstock heralding the beginning of the end of the sixties that sticks in my mind. It was a visit I made to Cambridge to meet a "bloke who is starting a new project to study some sort of worm", as my head of department at the Medical Research Council's National Institute of Medical Research informed me...

Reuben M et al. (2002) Dev Biol "Germline X chromosomes exhibit contrasting patterns of histone H3 methylation in Caenorhabditis elegans."

Reuben M, Lin R

[Dev Biol, 2002]

In mammals, one of the two somatic X chromosomes in the female is inactivated, thereby equalizing X chromosome-derived transcription in the two sexes, a process known as dosage compensation. In the germline, however, the situation is quite different. Both X chromosomes are transcriptionally active during female oogenesis, whereas the X and Y chromosomes are transcriptionally silent during male spermatogenesis. Previous studies suggest that Caenorhabditis elegans germline X chromosomes might have different transcriptional activity in the two sexes in a manner similar to that in mammals. Using antibodies specific to H3 methylated at either lysine 4 or lysine 9, we show that the pattern of site-specific H3 methylation is different between X chromosomes and autosomes as well as between germline X chromosomes from the two sexes in C. elegans. We show that the pachytene germline X chromosomes in both sexes lack Me(K4)H3 when compared with autosomes, consistent with their being transcriptionally inactive. This transcriptional inactivity of germline X chromosomes is apparently transient in hermaphrodites because both X chromosomes stain brightly for Me(K4)H3 after germ nuclei exit pachytene. The male single X chromosome, on the other hand, remains devoid of Me(K4)H3 staining throughout the germline. Instead, the male germline X chromosome exhibits a high level of Me(K9)H3 that is not detected on any other chromosomes in either sex, consistent with stable silencing of this chromosome. Using mutants defective in the sex determination pathway, we show that X-chromosomal Me(K9)H3 staining is determined by the sexual phenotype, and not karyotype, of the animal. We detect a similar high level of Me(K9)H3 in male mouse XY bodies, suggesting an evolutionarily conserved mechanism for silencing the X chromosome specifically in the male germline.

JB Stevenson et al. (1999) International C. elegans Meeting "How does the pharyngeal primordium generate different pharyngeal cell types?"

JB Stevenson, ME Domeier, A Chisholm, SE Mango

[International C. elegans Meeting, 1999]

Formation of the pharynx consists of three phases: i) specification of pharyngeal precursors during early embryogenesis, ii) assembly of these precursors into a pharynx primordium, and iii) terminal differentiation of the five pharyngeal cell types and morphogenesis of the pharynx into its adult form. Establishment of the pharyngeal precursors requires the pha-4 gene, which encodes a transcription factor expressed in all cells of the pharynx primordium. In a pha-4 mutant background, no or few pharyngeal cells are generated 1. How are five different pharyngeal cell types produced from an apparently homogeneous population of pharyngeal precursor cells? And what role does pha-4 play in this process, given its pan-pharyngeal expression pattern? To address these questions we are studying how one pharyngeal cell type, the marginal cell, is established during development. We have identified an early marker of marginal cells, namely the paired domain protein PAX-9. C. elegans pax-9::GFP is expressed in all nine marginal cells as well as three other pharyngeal cells. Deletion analysis has tentatively identified a region between -424 and -155 from the translational start site that is essential for appropriate pax-9 expression. Further deletion analysis is underway to identify potential cis elements within this region that are required for expression of pax-9 . In addition, we are characterizing the phenotypes associated with ectopic pax-9 and pax-9(RNAi) to learn the function of pax-9 during marginal cell development. 1. Mango et al ., Development 1994; Horner et al ., Genes and Development 1998, Kalb et al ., Development 1998.