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[
Worm Breeder's Gazette,
1994]
mab-3 YAC rescue David Zarkower, Mario de Bono, and Jonathan Hodgkin MRC Laboratory of Molecular Biology, Cambridge, England
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[
BMC Biol,
2018]
David Weinkove is an associate professor at Durham University, UK, studying host-microbe interactions in the model organism Caenorhabditis elegans. David has been focusing on the way microbes affect the physiology of their hosts, including the process of aging. In this interview, he discusses the questions shaping his research, how they evolved over the years, and his guiding principles for leading a lab.
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[
Worm Breeder's Gazette,
1992]
unc-4 LacZ expression in A-type motor neurons David M. Miller and Charles J. Niemeyer, Dept. of Cell Biology, Duke Univ. Medical Ctr, Durham, NC 27710
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[
Worm Breeder's Gazette,
1993]
DIFFERENTIAL EFFECTS OF DAUER-DEFECTIVE MUTATIONS ON L1- SPECIFIC SURFACE ANTIGEN SWITCHING. David G. Grenache and Samuel M. Politz, Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA.
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[
Worm Breeder's Gazette,
1994]
Strain names for non-C. elegans species Scott W. Emmonst, Armand Leroit, and David Fitch, Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461, Department of Biology, New York University, RmlOO9 Main Bldg., Washington Square, New York, NY 10003
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[
Worm Breeder's Gazette,
1994]
Cytology of degenerin-induced cell death in the PVM neuron David H. Hall, Guoqiang Gu+, Lei Gong#, Monica Driscoll#, and Martin Chalfie+, * Dept. Neuroscience, Albert Einstein College of Medicine, Bronx, N.Y. 10461 + Dept. Biological Sciences, Columbia University, New York, N.Y. 10027 # Dept. Molecular Biology and Biochemistry, Rutgers University, Piscataway, N.J. 08855
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[
J Vis Exp,
2017]
Next generation sequencing (NGS) technologies have revolutionized the nature of biological investigation. Of these, RNA Sequencing (RNA-Seq) has emerged as a powerful tool for gene-expression analysis and transcriptome mapping. However, handling RNA-Seq datasets requires sophisticated computational expertise and poses inherent challenges for biology researchers. This bottleneck has been mitigated by the open access Galaxy project that allows users without bioinformatics skills to analyze RNA-Seq data, and the Database for Annotation, Visualization, and Integrated Discovery (DAVID), a Gene Ontology (GO) term analysis suite that helps derive biological meaning from large data sets. However, for first-time users and bioinformatics' amateurs, self-learning and familiarization with these platforms can be time-consuming and daunting. We describe a straightforward workflow that will help C. elegans researchers to isolate worm RNA, conduct an RNA-Seq experiment and analyze the data using Galaxy and DAVID platforms. This protocol provides stepwise instructions for using the various Galaxy modules for accessing raw NGS data, quality-control checks, alignment, and differential gene expression analysis, guiding the user with parameters at every step to generate a gene list that can be screened for enrichment of gene classes or biological processes using DAVID. Overall, we anticipate that this article will provide information to C. elegans researchers undertaking RNA-Seq experiments for the first time as well as frequent users running a small number of samples.
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[
Science,
2002]
As any homeowner knows, timely maintenance is vital for keeping a building functioning properly after construction is finished. The same is evidently true for the complex architecture of the nervous system - at least in the roundworm. On page 686, neuroscientists Oliver Hobert, Oscar Aurelio, and David Hall describe a new family of proteins that help keep the wiring of the worm's nervous system tangle free.
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Munro, Edwin, Zaidel-Bar, Ronen, Ray, Shinjini, Yde, Sarah E., Kovar, David, Kadzik, Rachel S.
[
International Worm Meeting,
2021]
The dynamic actin cytoskeleton is continually remodeled during organism development, assembling and disassembling functionally diverse filamentous (F-) actin networks at precisely the right time and place. A major question is how a cell can organize and maintain multiple F-actin networks with diverse architectures and dynamics from a common pool of actin and actin-binding proteins (ABPs) within a single cytoplasm. We use the C. elegans zygote to study this question in vivo, and purified C. elegans proteins to reconstitute cytoskeletal dynamics in vitro. We hypothesize that a series of self-organization mechanisms facilitates the differential recruitment and activation of ABPs that determine actin filament architectures and dynamics of different F-actin networks. A key determinant of F-actin dynamics and architecture is the length of filaments within a network. A powerful regulator of actin filament length is the ABP capping protein (CP), which binds the fast-growing barbed ends of actin filaments to prevent polymerization and depolymerization. Using both 'bulk' and single-molecule/filament assays,we have biochemically characterized the dynamics of C. elegans ceCP on actin filament barbed ends in vitro. We find that ceCP has a high affinity for actin filament barbed ends and a slow off-rate relative to the lifetime of an actin filament in a C. elegans zygote. To characterize the biological activity of ceCP, we compared this in vitro data to the dynamics of ceCP in the C. elegans zygote using a powerful in vivo single-molecule approach. We found that the lifetime of ceCP single molecules on barbed ends in a zygote is much shorter than in vitro, suggesting the presence of regulatory mechanisms that act on ceCP in the zygote. We also characterize ceCP perturbation phenotypes at both the whole-network and single-filament level, and determine how ceCP regulates the balance of actin assembly and contributes to the self-sorting of other ABPs to different F-actin networks. Upon depletion of ceCP in a zygote, assembly of some F-actin networks is increased, while others are diminished, indicating a role for ceCP in the proper distribution of actin amongst networks. Additionally, we are characterizing other ABPs using similar combined in vitro/in vivo approaches, with the ultimate goal of using the C. elegans zygote to determine the minimal components for self-organization of multiple distinct F-actin networks within a common cytoplasm.
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[
Parasitol Today,
1996]
Historically, peptidergic substances (in the form of neurosecretions) were linked to moulting in nematodes. More recently, there has been a renewal of interest in nematode neurobiology, initially triggered by studies demonstrating the localization of peptide immunoreactivities to the nervous system. Here, David Brownlee, Ian Fairweather, Lindy Holden-Dye and Robert Walker will review progress on the isolation of nematode neuropeptides and efforts to unravel their physiological actions and inactivation mechanisms. Future avenues for research are suggested and the potential exploitation of peptidergic pathways in future therapeutic strategies