Simske JS et al. (1994) Worm Breeder's Gazette "Specification of Vulval Cell Fates By Sequential Signalling Pathways."

Simske JS, Kim SK

[Worm Breeder's Gazette, 1994]

Specification of vulval cell fates by sequential signaling pathways Jeffrey S. Simske and Stuart K. Kim, Dept. of Developmental Biology, Stanford University Medical Center, Stanford CA 94305

Plenefisch JD et al. (1994) Worm Breeder's Gazette "Cloning mua-3: Some Observations on the New Molecular Era"

Plenefisch JD, Hedgecock EM

[Worm Breeder's Gazette, 1994]

Cloning mua-3: some observations on the new Molecular Era John Plenefisch and Edward Hedgecock, Dept. of Biology, Johns Hopkins University, Baltimore MD 21218

Parham C et al. (1994) Worm Breeder's Gazette "Tc4 and Tc5: What Makes Them Move and Why It Matters"

Beinhorn J, Collins JJ, Butze K, Parham C

[Worm Breeder's Gazette, 1994]

Tc4 and Tc5: what makes them move and why it matters Christi Parham, Kristie Butze, Joanna Beinhorn and John Collins. Dept. of Biochemistry and Molecular Biology, University of New Hampshire. Durham, NH 03824

Almeida CA et al. (1994) Worm Breeder's Gazette "Function of a Domain of the Myosin Heavy Chain Implicated in Familial Hypertrophic Cardiomyopathy"

Collins JJ, Almeida CA, Swift KE

[Worm Breeder's Gazette, 1994]

Function of a Domain of the Myosin Heavy Chain Implicated in Familial Hypertrophic Cardiomyopathy Craig A. Almeida, Kerry E. Swift and John J. Collins Department of Biochemistry and Molecular, University of New Hampshire, Durham, NH 03820

Maine EM et al. (1994) Worm Breeder's Gazette "Mutations That Enhance glp-1 Identify Genes Required For Various Aspects of Germline Development."

Gelber MB, Smardon AM, Shu P, Qiao L, Maine EM, Lissemore JL

[Worm Breeder's Gazette, 1994]

Mutations that enhance glp-1 identify genes required for various aspects of germline development. Eleanor Maine, Li Qiao, Jim Lissemore-, Pei Shu, Anne Smardon, and Melanie Gelber. Biology Dept., Syracuse University, Syracuse, NY 13244 and Biology Dept., John Carroll University, Cleveland, OH 44118.

Otsuka AJ (1990) Worm Breeder's Gazette "ISOLATION AND CHARACTERIZATION OF UNC-104 CDNA CLONES"

Otsuka AJ

[Worm Breeder's Gazette, 1990]

Although the unc-104 fragments isolated from an Arhinger-Kimble library were instrumental in obtaining approximately 4 kb of the 6 kb mRNA sequence, it was desirable to obtain full length cDNA clones to both complete the cDNA sequence and to produce the recombinant protein product in bacteria. The discrepancy between the abundance of unc-104 cDNA clones in the Arhinger-Kimble library (approximately 1 per 10,000) and in the Kim-Horvitz (none in 200,000) and Thacker-Capecci libraries (none in 100,000), led us to screen the Barstead-Waterston and Schauer-Wood libraries. The abundance of cDNA clones in these additional libraries suggests that an insufficient number of clones was screened in the cases of the Kim and Thacker libraries. cDNA clones were isolated from 2.6 million phage (1.4 million phage screened from the Barstead-Waterston bank, 0.6 million the Schauer- Wood early embryonic and him-8 embryonic libraries). The probe was the 5'-most cDNA fragment obtained from the library. The results are shown below: [See Figure 1] The two cDNA clones from the Barstead library overlap and cover a region of 4.7 kb. DNA sequencing of the cDNA clones is currently in progress.

Li WQ et al. (1992) Worm Breeder's Gazette "Characterization of the Axonal Guidance and Outgrowth gene Unc-33"

Shaw JE, Li WQ, Herman RK

[Worm Breeder's Gazette, 1992]

Characterization of the axonal guidance and outgrowth gene unc-33 W. Li, R. K. Herman and J. E. Shaw Department of Genetics and Cell biology, University of Minnesota, St Paul, MN 55108

Greenwald IS (1984) Worm Breeder's Gazette "molecular cloning of lin-12."

Greenwald IS

[Worm Breeder's Gazette, 1984]

I have examined a total of eight independent lin-12 null mutations that arose spontaneously in a predominantly Bergerac genetic background. I probed genomic Southern blots with a 1.2 kb wild-type HindIII fragment that was defined by an insertion mutation (see last Newsletter) and/or with a cosmid 'contig' obtained from Alan Coulson and John Sulston that extends approximately 20 kb in each direction from the 1.2 kb HindIII fragment. The results thus far indicate that ( 1) seven of the eight mutations are associated with Tc1 insertions (2) the insertions are at different sites, and (3) all the insertion sites may lie within a single Pst fragment that is 2.8 kb in wild-type.

Uekusa T et al. (1994) Worm Breeder's Gazette "Molecular Analysis of unc-103"

Nishiwaki K, Miwa J, Uchida MK, Uekusa T

[Worm Breeder's Gazette, 1994]

Molecular analysis of unc-103 Tomoyuki Uekusa (uekusa@exp.cl.nec.cojp)l 2, Kiyoji Nishiwakil, Masaatsu K. Uchida2, and Johji Miwa1 1Fundamental Research Laboratories, NEC Corporation, Miyukigaoka, Tsukllba 305, Japan 2Department of Molecular Pharmacology, Meiji College of Pharmacy; 1-35-23, Setagaya- ku, Tokyo 154, Japan.

Adams A (1999) Worm Breeder's Gazette "Philadelphia's Northeast High School Worm Project experiment on the space shuttle Discovery"

Adams A

[Worm Breeder's Gazette, 1999]

We recently had a chance to do something only a handful of people have ever done: send an experiment up into space. We sent up an 'experimental' group of dauer larvae on the space shuttle Discovery's ten-day mission. ITA of Exton, PA, granted us a very small space on the shuttle. So we decided on the relatively small C. elegans. We figured since John Glenn was going up into space to see the effects on him, we'd do a similar study. We wanted to see if the conditions of space had any effect on the C. elegans normal 18-24 day life span. With every science experiment there is a control group and an experimental group. The control group of about 1000 worms stayed in Philadelphia at Northeast High School the other 1000 worms were blasted off into orbit October 29, 1998.